Production of Trout Offspring from Triploid Salmon Parents

Okutsu, Tomoyuki; Shikina, Shinya; Kanno, Megumi; Takeuchi, Yutaka; Yoshizaki, Goro

doi:10.1126/science.1145626

Cited by 245 publications

(185 citation statements)

References 4 publications

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“…Gonia within a testis (spermatogonia) or an ovary (oogonia) contain germ stem cells capable of self-renewal and differentiation even in culture (Hong et al, 2004). Transplantation data in fish support that gonia are sexually indeterminate or plastic (Okutsu et al, 2006;Okutsu et al, 2007;Yoshizaki et al, 2010). However, there is no evidence for the absence or presence of gonial sex (Kocer et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Germ cell sex prior to meiosis in the rainbow trout

Li¹,

Shen²,

Wong³

et al. 2011

Protein Cell

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Germ cells make two major decisions when they move from an indeterminate state to their final stage of gamete production. One decision is sexual commitment for sperm or egg production, and the other is to maintain mitotic division or entry into meiosis. It is unclear whether the two decisions are made as a single event or separate events, because there has been no evidence for the presence of germ cell sex prior to meiosis. Here we report direct evidence in the fish rainbow trout that gonia have distinct sexuality. We show that dazl expression occurs in both male and female gonia but exhibits differential intracellular distribution. More strikingly, we show that boule is highly expressed in male gonia but absent in female gonia. Therefore, mitotic gonia possess sex, sperm/egg decision and mitosis/meiosis decision are two independent events, and sperm/egg decision precedes mitosis/meiosis decision in rainbow trout, making this organism a unique vertebrate model for mechanistic understanding of germ cell sex differentiation and relationship between the two decisions.

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Section: Introductionmentioning

confidence: 99%

Germ cell sex prior to meiosis in the rainbow trout

Li¹,

Shen²,

Wong³

et al. 2011

Protein Cell

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“…This approach represents a significant development for germline transmission of cultured cells, and will be a potential reproduction technology to combat infertility in vertebrates including human. Additionally, primordial germ cells (PGCs) and spermatogonial stem cells could be transplanted between two different salmonid species, and resulted in production of donor-derived offspring [45][46][47]. In combination with natural and artificial polyploid fishes with special reproduction modes [18,48], the surrogate approach might have significant values for producing polyploidy stocks in aquaculture or for restoring an endangered species in wild life conservation.…”

Section: Somatic Cell Nuclear Transfer and Stem Cell Technologies In mentioning

confidence: 99%

Molecular basis and genetic improvement of economically important traits in aquaculture animals

2012

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Aquaculture has been believed to be a major Chinese contribution to the world. In recent 20 years, genome and other genetic technologies have promoted significant advances in basic studies on molecular basis and genetic improvement of aquaculture animals, and complete genomes of some main aquaculture animals have been sequenced or announced to be sequenced since the beginning of this century. Here, we review some significant breakthrough progress of aquaculture genetic improvement technologies including genome technologies, somatic cell nuclear transfer and stem cell technologies, outline the molecular basis of several economically important traits including reproduction, sex, growth, disease resistance, cold tolerance and hypoxia tolerance, and present a series of candidate trait-related genes. Finally, some application cases of genetic improvement are introduced in aquaculture animals, especially in China, and several development trends are highlighted in the near future.

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“…Saito et al (2011) have shown that a single PGCs transplantation technique works even between marine and freshwater species. Okutsu et al (2007) have also produced germline chimeras by transplanting rainbow trout (Oncorhynchus mykiss) spermatogonia from adult testis into sterile hatched larvae of masu salmon (O. masou), and they clearly showed that the germline chimeras generated only gametes of the donor. On the other hand, a transplantation technique has been developed both in tilapia and atherinopsid species, respectively, where spermatogonia from a donor were inserted directly into an adult recipient testis via genital pore (Majhi et al 2009;Lacerda et al 2010).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, it is difficult to prepare PGCs in the amount appropriate for transplantation. Regarding the spermatogonia transplantation by Okutsu et al (2007), Majhi et al (2009) or Lacerda et al (2010, although numerous cells can be prepared from an adult fish, the efficiency of germline chimerism is low.…”

Section: Introductionmentioning

confidence: 99%

Early embryonic development in pikeperch (Sander lucioperca) related to micromanipulation

Güralp¹,

Pocherniaieva²,

Blecha³

et al. 2016

CZECH J ANIM SCI

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ABSTRACT:Recently, transplantation of germ cells has attracted attention as a potential technique for efficient reproduction of fish. One of the well-proven techniques to deliver donor germ cells into a recipient is the transplantation of primordial germ cells (PGCs) during the blastula stage. Nevertheless, the application of such techniques so far has been limited to model fish species such as zebrafish, due to the lack of information about early development in many fish species. We propose that pikeperch (Sander lucioperca) can be a useful model species for establishing this technique in the order Perciformes, which includes commercially and ecologically important marine species. In this study, we described the important events, namely, embryonic staging, yolk syncytial layer (YSL) formation, and midblastula transition (MBT) during the blastula stage in pikeperch to obtain basic information about early embryonic development. The chorion was softened by treating with 0.2% trypsin and 0.4% urea in Ringer's solution so as to remove it easily by forceps. Although the first cleavage occurred at about 2.5 h post fertilization, blastomeres divided approximately every one hour after this at 15°C. The YSL was formed after the breakdown of marginal cells during the 512-to 1k-cell stage. Cell division analysis by 4'-6-diaminido-2-phenylindole (DAPI) staining revealed that transition from synchronous to asynchronous division occurred after the 10 th cleavage (1k-cell stage). Our results indicate that zygotic gene expression (MBT) starts after this stage. Next, we performed blastodisc isolation assay to find the competent stage for embryonic manipulation. Embryos were manipulated by using a microneedle every hour from the 512-cell to the sphere stage, and then developmental rates were evaluated at the hatching stage. The highest survival rate was obtained when we performed this manipulation at the 1k-cell stage. These results clearly showed that the MBT is the best stage for transplantation of PGCs or any cells in pikeperch.

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Production of Trout Offspring from Triploid Salmon Parents

Cited by 245 publications

References 4 publications

Germ cell sex prior to meiosis in the rainbow trout

Germ cell sex prior to meiosis in the rainbow trout

Molecular basis and genetic improvement of economically important traits in aquaculture animals

Early embryonic development in pikeperch (Sander lucioperca) related to micromanipulation

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