PRODUCTION OF Vi ANTIGEN ON A CHEMICALLY DEFINED MEDIUM BY A COLIFORM BACTERIUM

Jarvis, F. G.; Mesenko, Marianne T.; Tibbs, K. E.

doi:10.1128/jb.80.5.673-676.1960

Cited by 9 publications

(6 citation statements)

References 11 publications

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“…The latter requirement differs from the previous findings of Jarvis et al (5) with strain 10053. These workers found that glutamate stimulated antigen formation with no significant effect on growth.…”

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confidence: 99%

Nutritional Requirements of Arizona, Citrobacter , and Providencia

Machtiger

O’Leary

1971

J Bacteriol

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The nutritional requirements of Arizona, Citrobacter, and Providencia are compared, and chemically defined media in which these organisms may be grown are reported. Although certain enteric bacteria, such as Escherichia coli and various species of Salmonella, have been the subjects of countless investigations, little is known about the chemical composition or physiology of other members of the Enterobacteriaceae. In the course of studies on the chemical composition of the paracolons Arizona, Citrobacter, and Providencia, we wished to grow these organisms on defined media. This led us to study the nutritional requirements of Arizona, Citrobacter, and Providencia. The organisms we have studied have been the subjects of a number of investigations by others (4, 5, 8, 10), but in most of these studies bacteria have been grown on semidefined or complex media. Only in the case of the work by Jarvis et al. with Citrobacter (5) were cells grown on a chemically defined medium. Since we wished to standardize growth conditions as much as possible, we investigated the nutritional requirements of Arizona, Citrobacter, and Providencia. Cultures of Arizona, Citrobacter, P. alcalifaciens, and P. stuartil were acquired from a

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confidence: 99%

Nutritional Requirements of Arizona, Citrobacter , and Providencia

Machtiger

O’Leary

1971

J Bacteriol

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“…freundii was grown on a defined broth medium containing per liter: glucose, 7.5 g; sodium L-glutamate, 0.02 g; calcium pantothenate, 0.0001 g; (NH4)2SO4, 1.25 g; KH2PO4, 3.0 g; Na2HPO4, 6.0 g; MgSO4-7H20, 0.30 g; and NaCl, 5.0 g. The experimental basis for the use of this medium and the cultural conditions employed have been reported (Jarvis, Mesenko, and Tibbs, 1960). S. typhosa was grown on veal infusion agar (Difco).…”

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confidence: 99%

ELECTROPHORETIC PURIFICATION OF THE Vi ANTIGEN

1960

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“…Antigenz preparations. The Vi antigen was prepared and purified as previously described (6,7) and was preserved by lyophilization of its sodium salt. The alkali-hydrolyzed form was prepared by treatment of unaltered antigen with 0.1 N NaOH at room temperature for 2 hr, precipitation with mineral acid, dialysis of the sodium salt, precipitation of the salt form with acetone, and lyophilization from aqueous solution at pH 7.0.…”

Section: Methodsmentioning

confidence: 99%

“…Proc., p. 131, 1961). The antigen was obtained from Escherichia freundii 5396/38 grown on a shaken, chemically defined medium (7). Sedimentation, diffusion, viscosity, and infrared absorption measurements are reported I Present address: 329 Riverview, 6W, Great Falls,…”

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Physicochemical Properties of the Vi Antigen Before and After Mild Alkaline Hydrolysis

et al. 1967

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Sedimentation and diffusion constants were determined for electrophoretically purified Vi antigen before and after mild alkaline hydrolysis. The molecular weight of the intact antigen was found to be 1.7 X 106. Mild alkaline hydrolysis completely removed 0-acetyl, reduced the molecular weight to 1.0 x 105, and lowered the intrinsic viscosity from 8.0 deciliters/g to approximately 0.5 deciliter/g. Infrared spectra of the two antigen preparations were also compared.

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PRODUCTION OF Vi ANTIGEN ON A CHEMICALLY DEFINED MEDIUM BY A COLIFORM BACTERIUM

Cited by 9 publications

References 11 publications

Nutritional Requirements of Arizona, Citrobacter , and Providencia

Nutritional Requirements of Arizona, Citrobacter , and Providencia

ELECTROPHORETIC PURIFICATION OF THE Vi ANTIGEN

Physicochemical Properties of the Vi Antigen Before and After Mild Alkaline Hydrolysis

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