Production of xylooligosaccharides from corncob xylan by fungal xylanase and their utilization by probiotics

Chapla, Digantkumar; Pandit, Pratima; Shah, Amita

doi:10.1016/j.biortech.2011.10.083

Cited by 279 publications

(138 citation statements)

References 30 publications

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“…Xylanases from A. caespitosus remained stable in the pH range of 5-7 (35). On the other hand, the best xylanase activity produced by A. foetidus MTCC 4898 was at pH=5.3, but decreased by 34 and 50 % at pH=4.5 and 6.0, respectively (36). The xylanases from A. fumigatus M51 and A. fumigatus U2370 showed higher thermostability at 10 to 50 °C for 1 to 3 h of incubation than the xylanase from T. reesei CCT 2768 (10 to 40 °C).…”

Section: Discussionmentioning

confidence: 87%

Screening of Xylanolytic Aspergillus fumigatus for Prebiotic Xylooligosaccharide Production Using Bagasse

Carvalho

Neto

Almeida

et al. 2015

Food Technol. Biotechnol.

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Currently, lignocellulosic waste is a topic of global studies, given that fossil fuel reserves are diminishing, the new agricultural frontiers are limited and the demand for food and biofuels is increasing by the growing world population (1). For these reasons, technology must advance to improve the use of agricultural and agroindustrial residues to obtain food and biofuel.Xylans present in lignocellulosic materials have been studied for obtaining xylooligosaccharides (XOS) from waste materials such as corncobs, rice hulls, olive pits, barley straw (2), tobacco stalk, cott on stalk, sunfl ower stalk, wheat straw (3) and sugarcane bagasse (4-6). Sugarcane bagasse is inexpensive, renewable and abundant source of XOS especially in the countries that produce ethanol and sugar from sugarcane. However, more research is necessary to improve the use of this residue. SummarySugarcane bagasse is an important lignocellulosic material studied for the production of xylooligosaccharides (XOS). Some XOS are considered soluble dietary fi bre, with low caloric value and prebiotic eff ect, but they are expensive and not easily available. In a screening of 138 fungi, only nine were shortlisted, and just Aspergillus fumigatus M51 (35.6 U/mL) and A. fumigatus U2370 (28.5 U/mL) were selected as the most signifi cant producers of xylanases. These fungi had low β-xylosidase activity, which is desirable for the production of XOS. The xylanases from Trichoderma reesei CCT 2768, A. fumigatus M51 and A. fumigatus U2370 gave a signifi cantly higher XOS yield, 11.9, 14.7 and 7.9 % respectively, in a 3-hour reaction with hemicellulose from sugarcane bagasse. These enzymes are relatively thermostable at 40-50 °C and can be used in a wide range of pH values. Furthermore, these xylanases produced more prebiotic XOS (xylobiose and xylotriose) when compared with a commercial xylanase. The xylanases from A. fumigatus M51 reached a high level of XOS production (37.6 %) in 48-72 h using hemicellulose extracted from sugarcane bagasse. This yield represents 68.8 kg of prebiotic XOS per metric tonne of cane bagasse. In addition, in a biorefi nery, aft er hemicellulose extraction for XOS production, the residual cellulose could be used for the production of second-generation ethanol.

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Section: Discussionmentioning

confidence: 87%

Screening of Xylanolytic Aspergillus fumigatus for Prebiotic Xylooligosaccharide Production Using Bagasse

Carvalho

Neto

Almeida

et al. 2015

Food Technol. Biotechnol.

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“…Although XOS did not support optimum growth and acid production, B. longum consumed large amounts of XOS, suggesting that these bacteria, despite extensive hydrolytic release of xylose, could not efficiently utilize this sugar for growth and fermentation into acid. L. acidophilus did not efficiently degrade XOS, perhaps reflecting differences in expression of β-xylosidase, which is needed for utilization of XOS [49]. B. longum consumed only sparse amounts of CBS, the major cello-oligosaccharide, presumably due to lack of specific β-1,4-glucosyl hydrolase activity.…”

Section: Discussionmentioning

confidence: 94%

Relative fermentation of oligosaccharides from human milk and plants by gut microbes

Wang

Chen

et al. 2016

Eur Food Res Technol

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sparse. B. longum fermentation products inhibited C. jejuni and E. coli. Thus, HMOS most strongly promoted growth of the two mutualists, and both HMOS and GMOS were efficiently fermented by these mutualists into organic acids. This is consistent with a primary role of HMOS in guiding early colonization of the infant microbiota by mutualist symbionts, and of plant oligosaccharides, especially GMOS, in maintaining a favorable microbiota through adulthood.Keywords Human milk oligosaccharides · Plant oligosaccharides · Microbiota · Prebiotic · Organic acids Abbreviations HMOSHuman milk oligosaccharides GMOS Gluco-manno-oligosaccha-Abstract Gut microbiota is important to human health. Specific dietary glycans promote favorable microbiota growth and inhibit pathobionts. Dietary glycans most relevant to adults and weaned infants are derived from plants or lactose; human milk oligosaccharides (HMOS) are most relevant to breastfed infants. Their efficacy in supporting bacterial growth is compared to determine their potential roles in the initiation and maintenance of colonization. Bioactivities of gluco-manno-oligosaccharides (GMOS), galacto-oligosaccharides (GOS), xylo-oligosaccharides (XOS), cellobiose (CBS), HMOS, and the most prominent individual HMOS, 2′-fucosyllactose (2′-FL) were contrasted. Two representative gut microbiota mutualists, Bifidobacteria longum ATCC15697 and Lactobacillus acidophilus NRRL B-4495, and two non-mutualists, Campylobacter jejuni S107 and Escherichia coli K12, were used to assess the in vitro prebiotic potential of these oligosaccharides. All oligosaccharides afforded growth of B. longum and L. acidophilus, with HMOS supporting the most robust growth, while none of these oligosaccharides afforded meaningful growth of non-mutualists. B. longum efficiently converted HMOS, GMOS, GOS, and XOS into organic acid fermentation products, and, to a lesser degree, L. acidophilus metabolized HMOS, GMOS, and GOS. Fermentation of these glycans by C. jejuni and E. coli was

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“…cellobiosus) (14,21) and Lb. acidophilus (15) were found to have moderate grow rates on XOS, and Chapla et al (43) reported that growth of Lb. fermentum and Lb.…”

Section: Discussionmentioning

confidence: 99%

Arabinoxylan Oligosaccharide Hydrolysis by Family 43 and 51 Glycosidases from Lactobacillus brevis DSM 20054

Michlmayr

Hell

Lorenz

et al. 2013

Appl Environ Microbiol

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Due to their potential prebiotic properties, arabinoxylan-derived oligosaccharides [(A)XOS] are of great interest as functional food and feed ingredients. While the (A)XOS metabolism of Bifidobacteriaceae has been extensively studied, information regarding lactic acid bacteria (LAB) is still limited in this context. The aim of the present study was to fill this important gap by characterizing candidate (A)XOS hydrolyzing glycoside hydrolases (GHs) identified in the genome of Lactobacillus brevis DSM 20054. Two putative GH family 43 xylosidases (XynB1 and XynB2) and a GH family 43 arabinofuranosidase (Abf3) were heterologously expressed and characterized. While the function of XynB1 remains unclear, XynB2 could efficiently hydrolyze xylooligosaccharides. Abf3 displayed high specific activity for arabinobiose but could not release arabinose from an (A)XOS preparation. However, two previously reported GH 51 arabinofuranosidases from Lb. brevis were able to specifically remove ␣-1,3-linked arabinofuranosyl residues from arabino-xylooligosaccharides (AXHm3 specificity). These results imply that Lb. brevis is at least genetically equipped with functional enzymes in order to hydrolyze the depolymerization products of (arabino)xylans and arabinans. The distribution of related genes in Lactobacillales genomes indicates that GH 43 and, especially, GH 51 glycosidase genes are rare among LAB and mainly occur in obligately heterofermentative Lactobacillus spp., Pediococcus spp., members of the Leuconostoc/Weissella branch, and Enterococcus spp. Apart from the prebiotic viewpoint, this information also adds new perspectives on the carbohydrate (i.e., pentose-oligomer) metabolism of LAB species involved in the fermentation of hemicellulose-containing substrates.

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Production of xylooligosaccharides from corncob xylan by fungal xylanase and their utilization by probiotics

Cited by 279 publications

References 30 publications

Screening of Xylanolytic Aspergillus fumigatus for Prebiotic Xylooligosaccharide Production Using Bagasse

Screening of Xylanolytic Aspergillus fumigatus for Prebiotic Xylooligosaccharide Production Using Bagasse

Relative fermentation of oligosaccharides from human milk and plants by gut microbes

Arabinoxylan Oligosaccharide Hydrolysis by Family 43 and 51 Glycosidases from Lactobacillus brevis DSM 20054

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