Production of zebrafish germ-line chimeras from embryo cell cultures

Ma, Chunguang; Fan, Lianchun; Ganassin, Rosemarie C.; Bols, Niels C.; Collodi, Paul

doi:10.1073/pnas.041449398

Cited by 154 publications

(95 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Ma et al [17] reported the production of zebrafish germline chimeras from short-term embryo cell cultures. However, attempts to produce germline chimeras from medaka ES cell cultures have so far been unsuccessful.…”

Section: Introductionmentioning

confidence: 99%

Accessibility of host cell lineages to medaka stem cells depends on genetic background and irradiation of recipient embryos

Hóng

Zeng

et al. 2010

Cell. Mol. Life Sci.

View full text Add to dashboard Cite

Chimera formation is a powerful tool for analyzing pluripotency in vivo. It has been widely accepted that host cell lineages are generally accessible to embryonic stem (ES) cells with the actual contribution depending solely on the intrinsic pluripotency of transplanted donor cells. Here, we show in the fish medaka (Oryzias latipes) that the host accessibility to ES cell contribution exhibits dramatic differences. Specifically, of three albino host strains tested (i 1 , i 3 and af), only strain i 1 generated pigmented chimeras. Strikingly, this accessibility is completely lost in i 1 but acquired in i 3 after host c-irradiation. Host irradiation also differentially affected ES cell contribution to somatic organs and gonad. Therefore, the accessibility of various host cell lineages can vary considerably depending on host strains and cell lineages as well as on irradiation. Our findings underscore the importance of host genotypes for interpreting donor cell pluripotency and for improving ES-derived chimera production.

show abstract

Section: Introductionmentioning

confidence: 99%

Accessibility of host cell lineages to medaka stem cells depends on genetic background and irradiation of recipient embryos

Hóng

Zeng

et al. 2010

Cell. Mol. Life Sci.

View full text Add to dashboard Cite

show abstract

“…In fish, first attempts have been made in small aquarium model species, for example, the zebrafish and medaka, because they offer a unique opportunity of combining embryological, genetic and molecular analyses of vertebrate development. Germline chimeras have been produced successfully from non-cultivated blastomeres of zebrafish (Lin et al, 1992) and medaka (Wakamatsu et al, 1993) and from short-term cultures of zebrafish embryonic cells (Ma et al, 2001). Fertile nuclear transplants have also been obtained from nuclei of medaka blastulae (Wakamatsu et al, 2001) and of zebrafish fibroblast cultures (Lee et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…Fertile nuclear transplants have also been obtained from nuclei of medaka blastulae (Wakamatsu et al, 2001) and of zebrafish fibroblast cultures (Lee et al, 2002). Much progress has been made towards the development of ES cells in medaka (Wakamatsu et al, 1994;Hong et al, , 1998a, zebrafish (Ma et al, 2001) as well as other fish species including the seabream (Bejar et al, 2002), red seabream (Chen et al, 2003a) and sea perch (Chen et al, 2003b). In particular, one medaka ES cell line, MES1, has proven to retain a diploid karyotype and the ability to form chimeric adults (Hong et al, , 1998a(Hong et al, , b, c, 2000(Hong et al, , 2003.…”

Section: Introductionmentioning

confidence: 99%

Retention of the Developmental Pluripotency in Medaka Embryonic Stem Cells after Gene Transfer and Long-term Drug Selection for Gene Targeting in Fish

et al. 2004

View full text Add to dashboard Cite

Embryonic stem (ES) cells provide a unique tool for introducing random or targeted genetic alterations, because it is possible that the desired, but extremely rare recombinant genotypes can be screened by drug selection. ES cell-mediated transgenesis has so far been limited to the mouse. In the fish medaka (Oryzias latipes) several ES cell lines have been made available. Here we report the optimized conditions for gene transfer and drug selection in the medaka ES cell line MES1 as a prelude for gene targeting in fish. MES1 cells gave rise to a moderate to high transfection efficiency by the calcium phosphate co-precipitation (5%), commercial reagents Fugene (11%), GeneJuice (21%) and electroporation (>30%). Transient gene transfer and CAT reporter assay revealed that several enhancers/promoters and their combinations including CMV, RSV and ST (the SV40 virus early gene enhancer linked to the thymidine kinase promoter) were suitable regulatory sequences to drive transgene expression in the MES1 cells. We show that neo, hyg or pac conferred resistance to G418, hygromycin or puromycin for positive selection, while the HSV-tk generated sensitivity to ganciclovir for negative selection. The positive-negative selection procedure that is widely used for gene targeting in mouse ES cells was found to be effective also in MES1 cells. Importantly, we demonstrate that MES1 cells after gene transfer and long-term drug selection retained the developmental pluripotency, as they were able to undergo induced differentiation in vitro and to contribute to various tissues and organs during chimeric embryogenesis.

show abstract

“…As a next step for germ-line chimera in fish species, several studies are performed for establishment of culture-cells, such as embryonic stem cell (ES cell), which can differentiate germ-line cells when they introduced into the host embryos (Wakamatsu et al, 1994;Ma et al, 2001;Fan et al, 2004). However, these studies have been performed in exclusive model-species, such as zebrafish and medaka, and still uncertain.…”

Section: Hetero-chronic Transplantation Of Gfp Fluorescence-visualizementioning

confidence: 99%

Developmental biotechnology for aquaculture, with special reference to surrogate production in teleost fishes

Yamaha

Saito

Goto-Kazeto

et al. 2007

Journal of Sea Research

View full text Add to dashboard Cite

The purpose of this review is to introduce surrogate production as a new technique for fish-seed production in aquaculture. Surrogate production in fish is a technique used to obtain the gametes of a certain genotype through the gonad of another genotype. It is achieved by inducing germ-line chimerism between different species during early development. Primordial germ cells (PGCs) are a key material of this technique to induce germ-line chimera. In several species, it has been reported that PGCs differentiated from the blastomeres inherited some maternally supplied mRNA located at the terminal regions of the early cleavage furrows. PGCs from donor species (or strains) are isolated and transplanted into host species to induce the germ-line chimera.Four methods for inducing germ-line chimera, namely, blastomere transplantation, blastoderm-graft transplantation, transplantation of PGC from the genital ridge and transplantation visualized PGC with GFP fluorescence, are described. Several problems preventing the successful induction of germ-line chimera in various fish species are mentioned. Surrogate production, however, has the possibility to allow efficient fish-seed production and effective breeding and transfer of biodiversity to an aquaculture strain.Conservation and efficient utilization of genetic resources will be achieved through surrogate production combined with the cryopreservation of PGCs.

show abstract

Production of zebrafish germ-line chimeras from embryo cell cultures

Cited by 154 publications

References 30 publications

Accessibility of host cell lineages to medaka stem cells depends on genetic background and irradiation of recipient embryos

Accessibility of host cell lineages to medaka stem cells depends on genetic background and irradiation of recipient embryos

Retention of the Developmental Pluripotency in Medaka Embryonic Stem Cells after Gene Transfer and Long-term Drug Selection for Gene Targeting in Fish

Developmental biotechnology for aquaculture, with special reference to surrogate production in teleost fishes

Contact Info

Product

Resources

About