Production of zinc-enriched biomass of Saccharomyces cerevisiae

Azad, S. Kamran; Shariatmadari, F.; Torshizi, Mohammad Amir Karimi

doi:10.5601/jelem.2014.19.2.655

Cited by 7 publications

(7 citation statements)

References 37 publications

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“…In the study of zn-enriched yeast, Shariatmadari et al (2014) added zinc salt to Saccharomyces cerevisiae culture medium to improve the yield of organic zinc. Therefore, the concentration of zinc sulfate in the medium has a significant effect on the zinc content and cell growth of yeast cells.…”

Section: Discussionmentioning

confidence: 99%

Screening and identification of yeast enriched with selenium, zinc and chromium

Li¹,

Shu²,

Yang³

et al. 2022

Acta Sci Pol Technol Aliment

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Background. Selenium zinc chromium is an essential nutrient for humans and animals, and the free form of selenium zinc chromium state is not easily absorbed and is highly toxic. Yeast has characteristics such as easy growth and a high absorption rate of trace elements, which make it an ideal carrier for enrichment of trace elements. Therefore, in order to solve the problems of the low absorption rate of trace elements and the difficulty of effective daily supplementation, this study will examine the use of yeast to enrich more than two kinds of nutrients at the same time and to obtain and identify the strain with a good enrichment effect, high biomass and a broad development prospect. Methods. Sixteen yeast strains were selected in order to study their enrichment capacity for selenium, zinc and chromium, respectively, using enrichment amount and conversion rate as screening indicators. The strains were then identified by sequence analysis of the 26S rDNA D1/D2 region. Results. The strain with a better enrichment effect of selenium, zinc and chromium was obtained as Saccharomyces boulardii L2. When the compound nutrients sodium selenite (30 μg/mL), zinc sulfate (200 μg/mL) and chromium chloride (100 μg/mL) were added to the medium, the selenium content of the yeast was obtained, and the zinc content and chromium content were 917.37 μg/g, 1202.3 μg/g, 680.11 μg/g, respectively. The biomass was 19.58 g/L. Conclusion. Fortified yeast enriched with selenium, zinc and chromium was obtained using S. boulardii L2 as a carrier. This study provides the theoretical basis and technical support for the preparation of yeast rich in selenium, zinc and chromium.

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Section: Discussionmentioning

confidence: 99%

Screening and identification of yeast enriched with selenium, zinc and chromium

Li¹,

Shu²,

Yang³

et al. 2022

Acta Sci Pol Technol Aliment

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“…Selain itu, zinc juga membantu sintesis senyawa alanin dan glutation yang berperan untuk keseimbangan proses metabolisme khususnya tahap glikolisis (Zhao & Bai, 2012). Zinc mampu meningkatkan sintesis riboflavin dan menstimulus terjadinya proliferasi sel sehingga kecepatan pertumbuhan khamir dapat meningkat (Azad et al, 2014). Zinc juga berperan sebagai kofaktor bagi enzim alkohol dehidrogenase pada proses fermentasi.…”

Section: Pendahuluanunclassified

“…Hasil tersebut juga berpengaruh terhadap hasil fermentasi dengan dihasilkan etanol sebanyak 110,8 g/L. Azad et al (2014) dalam penelitiannya menggunakan zinc sulfat sebesar 5-10 mg/L mendapatkan pertumbuhan lebih efisien dari konsentrasi lainnya yang ditandai dengan hasil Optical Density (OD) lebih tinggi. Laju pertumbuhan yang lebih tinggi pada konsentrasi 5 mg/L juga menghasilkan biomassa tertinggi sebesar 4.995 g/L.…”

Section: Pendahuluanunclassified

“…Penelitian sebelumnya terkait penambahan zinc sebagai mikronutrien tambahan untuk meningkatkan biomassa, terbatas pada jumlah biomassa yang didapatkan (Azad et al, 2014;Zhao et al, 2009). Berdasarkan penelitian pendahuluan, jumlah sel hidup khamir pada fermipan maupun sel hidup khamir pada biomassa, dapat memengaruhi kecepatan waktu fermentasi adonan roti.…”

Section: Pendahuluanunclassified

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Analisis Pertumbuhan Khamir Endofit Buah Salak (Salacca edulis Reinw.) dengan Penambahan Zinc

Kusmiyati,

Utami,

Pratama

et al. 2024

Al-Kauniyah J. Biol.

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AbstrakKhamir endofit buah salak (Salacca edulis Reinw.) dengan kode YIS-3, YIS-4, dan YIS-7 dapat dimanfaatkan untuk fermentasi adonan roti. Kemampuan tersebut dapat ditingkatkan dengan penambahan sumber zinc pada media pertumbuhannya. Tujuan penelitian ini untuk menganalisis pertumbuhan khamir endofit buah salak dengan penambahan zinc dan kualitas roti hasil fermentasi. Metode yang digunakan meliputi peremajaan isolat khamir, penambahan nutrisi pada media pertumbuhan, analisis biomassa khamir, jumlah sel, volume adonan, dan organoleptik. Hasil penelitian pada parameter pertumbuhan menunjukkan bahwa perlakuan dengan penambahan zinc 0,1 g/L menghasilkan biomassa dan jumlah sel lebih tinggi dibandingkan perlakuan kontrol. Biomassa tertinggi dihasilkan oleh YIS-4 yakni 4,13 g/300 mL, sedangkan jumlah sel tertinggi dihasilkan oleh YIS-3 yakni 27,84 x 106 sel/mL. Pada persentase pengembangan menunjukkan bahwa isolat khamir dengan perlakuan zinc 0,1 g/L membutuhkan waktu lebih lama untuk mencapai pengembangan tertinggi, namun volume roti setelah pemanggangan menunjukkan hasil yang lebih baik dibanding perlakuan kontrol. Isolat YIS-4 dengan perlakuan zinc 0,1 g/L menghasilkan volume roti tertinggi (949,54 cm3). Berdasarkan analisis organoleptik dengan parameter warna, aroma, rasa dan tekstur menunjukkan bahwa semua panelis lebih menyukai roti hasil fermentasi isolat YIS-4 dengan perlakuan zinc 0,1 g/L.AbstractThe endophytic yeast of salak fruit (Salacca edulis Reinw.) with codes YIS-3, YIS-4, and YIS-7 can be used for bread dough fermentation. This ability can be increased by adding a zinc source to the growth medium. The purpose of this study was to analyze the growth of the endophytic yeast of salak fruit with the addition of zinc and the quality of fermented bread. The methods used include rejuvenation of yeast isolates, addition of nutrients to growth media, analysis of yeast biomass, cell count, dough volume and organoleptic. The results of research on growth parameters showed that treatment with the addition of zinc 0.1 g/L resulted in higher biomass and cell count than the control treatment. The highest biomass was produced by YIS-4 which was 4.13 g/300 mL, while the highest number of cells was produced by YIS-3 which was 27.84 x 106 cells/mL. The swelling percentage showed that yeast isolates treated with 0.1 g/L zinc took longer to reach the highest swelling, but the volume of bread after baking showed better results than the control treatment. YIS-4 isolate treated with 0.1 g/L zinc produced the highest bread volume (949.54 cm3). Based on organoleptic analysis with parameters of color, aroma, taste and texture, it showed that all panelists preferred bread fermented YIS-4 isolate with 0.1 g/L zinc treatment.

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“…There are several reports (Bhosale, 2004;Buzzini et al, 2005;Marova et al, 2011) regarding the effect of supplementation of metal ions (Mg, Zn, Cu, Ca) in culture medium on metalsenriched biomass obtaining and stimulation of yeast growth and production of carotenoids. Saccharomyces strains were investigated for their ability to accumulate high concentrations of Cu, Zn and Mn salts to obtain ions enriched biomass (Barbulescu et al, 2009;Šillerová et al, 2012;Azad et al, 2014) and the "pink yeast" Rhodotorula glutinis to demonstrate the stimulatory effect of Ba, Fe, Mg, Ca, Zn, Co divalent cations on growth rate and volumetric production of carotenoids (Bhosale et al, 2001;Buzzini et al, 2005;Martínez et al, 2006;Dai et al, 2007). This unicellular yeast, wide-spread in nature, is well known to biosynthesize, in low (less than 100 μg/ g dry biomass) (Besarab et al, 2018), medium (101 up to 500 μg/g) and high (more than 500 μg/g dry biomass) (Buzzini et al, 2000;Issa et al, 2016) proportions, β-carotene, torulene and torularhodin, specific pigments of high economic value and great biotechnological importance.…”

Section: Introductionmentioning

confidence: 99%

THE EFFECT OF ZnSO4 IN THE CULTIVATION MEDIUM ON RHODOTORULA GLUTINIS CCY 020-002-033 YEAST BIOMASS GROWTH, β-CAROTENE PRODUCTION AND ZINC ACCUMULATION

Rovinaru¹,

Pasarin²,

Capră³

et al. 2018

J microb biotech food sci

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The goal of this work was to evaluate the effect of zinc sulphate heptahydrate on growth rate and β-carotene production and to obtain zinc-enriched biomass using selected yeast strain Rhodotorula glutinis CCY 020-002-033. The batch cultivation was performed on synthetic basal medium supplemented with various concentrations of ZnSO4·7H20 (0.025%, 0.035%, and 0.050%), in shake flasks and aerobic conditions, at 300C, for five days. Total zinc accumulated in yeast cells after fermentation period was performed using ICP-OES. The identification and yield of β–carotene were investigated by chromatographic analysis (HPLC). It was found that the amount of total zinc accumulated in yeast cells depends mainly on the zinc concentration in the culture medium. The highest zinc content of 7.030 mg/g dry biomass was obtained at optimal concentration of 0.025%, with an incorporation efficiency of 87%. High concentration of 0.050% was not tolerated by yeast. Zinc salt in concentration of 0.025%, acted as stimulant for volumetric β–carotene but not improve yeast growth biomass. β-Carotene maximum content in this strain’s cell mass accounted 7.1 mg/L, when the dry cell biomass (1.89 g/L) was smaller than control (1.94 g/L), although there was no significant difference observed in final dry cell biomass versus control. The obtained results showed that when ZnSO4·7H20 concentration was increased up to 0.050% a decrease in yeast growth rate, β–carotene production and zinc accumulation was observed.

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Production of zinc-enriched biomass of Saccharomyces cerevisiae

Cited by 7 publications

References 37 publications

Screening and identification of yeast enriched with selenium, zinc and chromium

Screening and identification of yeast enriched with selenium, zinc and chromium

Analisis Pertumbuhan Khamir Endofit Buah Salak (Salacca edulis Reinw.) dengan Penambahan Zinc

THE EFFECT OF ZnSO4 IN THE CULTIVATION MEDIUM ON RHODOTORULA GLUTINIS CCY 020-002-033 YEAST BIOMASS GROWTH, β-CAROTENE PRODUCTION AND ZINC ACCUMULATION

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