Production, optimization, and partial purification of lipase fromPseudomonassp. strainBUP6, a novel rumen bacterium characterized fromMalabari goat

Abstract

This study introduces a novel bacterium-Pseudomonas sp. strain BUP6-isolated from the rumen of the Malabari goat with efficiency for producing lipase. It showed significant production of lipase when grown in a newly designed basal medium, supplemented with vegetable oil. Suitability of five vegetable oils such as groundnut oil, coconut oil, olive oil, sunflower oil, and palm oil as inducer for the production of lipase was examined, and groundnut oil supported the highest production of lipase (96.15 U/mL). Various physical parameters required for the maximum production of lipase were statistically optimized. Plackett-Burmann design was employed to study the interactive effects of physical parameters and found that temperature, agitation, and pH effected the production of lipase significantly. The optimum conditions for lipase production (37 °C, 200 rpm, and pH 6.9) were detected by Box-Behnken design and response surface methodology, which resulted in the 0.3-fold increase (i.e., 126 U/mL) of the lipase activity over the unoptimized condition. The apparent molecular mass of partially purified lipase was 35 kDa, as judged by SDS-PAGE; the activity of lipase was also confirmed by native PAGE. Thus, this study focuses on the need for the exploitation of rumen microbes for the production of industrially significant and human-friendly biomolecules to meet the future needs.

Pseudomonas sp. BUP6, a novel isolate from Malabari goat produces an efficient rhamnolipid type biosurfactant

“…KF550910; MTCC No. 5925) was used in this study [14]. It was cultured in basal salt medium with the following ingredients (per liter): 5 g NH 4 NO 3 , 4 g (NH 4 ) 2 SO 4 , 3 g yeast extract, 2 g K 2 HPO 4 , 2 g NaCl, 0.01 g MgSO 4 .7H 2 O, 0.01 g CaCl 2 supplemented with 0.5% groundnut oil (supplied as emulsion in distilled water by sonication), and incubated at 37°C at 140 rpm [14].…”

Optimization of Parameters for the Production of Lipase from Pseudomonas sp. BUP6 by Solid State Fermentation

“…strain BUP6 (Genbank Accession No. KF 550910), isolated from the rumen of Malabari goat, was used for this study [9]. The bacterium was cultured on basal salt medium (BSM), supplemented with 0.3% of vegetable oil, and incubated at 37˚C for 24 h. The stock culture was maintained on BSM agar slants, which was sub-cultured in an interval of 2 weeks.…”

“…Production of lipase was qualitatively assayed by the method of by Priji et al [9]. Para-nitro phenyl palmitate (p-NPP) was used as substrate for lipase assay.…”

Microbial Lipases − Properties and Applications

“…For the past few decades, lipases have gained much attention due to their versatile activities toward extreme temperature, pH, organic solvents; and chemo-, regio-and enantio-selectivities (Benjamin & Pandey 1996). In contrast to esterases, lipases are activated only when adsorbed to an oil-water interface i.e., lipase possesses a unique property of catalyzing the hydrolysis of ester bonds at the interface between an insoluble substrate phase and the aqueous phase, where the enzyme remains dissolved (Benjamin & Pandey 2000; Priji et al 2014). For the past two decades, the world-wide production and consumption of microbial lipases have increased considerably owing to their fascinating industrial applications, which summarize the lipases as the third largest group of enzymes after proteases and amylases.…”