Production, purification and characterisation of a chitosanase from Bacillus cereus

Wang, Chuan Lu; Su, Jien Wei; Liang, Tzu-Wen; Nguyen, Anh Dzung; Wang, San-Lang

doi:10.1007/s11164-014-1601-1

Cited by 24 publications

(25 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For fungal antagonism, endophytic bacteria can release chitinases, beta glucanases, proteases, chemical compounds, and antibiotics, improving the environmental competitiveness [7][8][9]. In addition, endophytic bacteria have beneficial bioactivities such as nitrogen fixing, phosphorous solubility, and IAA biosynthesis to stimulate the growth of crops, reduce fertilizer application, and increase drought tolerance [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Phytophthora Antagonism of Endophytic Bacteria Isolated from Roots of Black Pepper (Piper nigrum L.)

et al. 2020

Self Cite

View full text Add to dashboard Cite

In this study, 90 root samples were collected from 30 black pepper farms in three provinces in the Central Highlands of Vietnam. A total of 352 endophytic bacteria were isolated and their morphology described. An in vitro assay on the antifungal activity of these isolates was then conducted and 47 isolates were found to have antagonistic activity on Phytophthora fungi. The antifungal activity of the 47 isolates was evaluated in vivo by shoot assay. Among these 47 isolates, 6 were selected for further investigation. The six isolates were classified and identified by sequencing the 16S RNA gene and phylogeny. The results showed that all six endophytic bacteria belong to the following species of Bacillus genus: B. siamensis, B. amyloliquefaciens, B. velezenis, and B. methylotrophiycus. Enzymatic activity related to the antifungal activity of the six potent isolates was determined; it showed that they possessed high chitinase and protease activities. These isolates were applied for black pepper seedlings in greenhouse. The results showed three promising isolates: B. siamensis EB.CP6, B. velezensis EB.KN12, and B. methylotrophycus EB.KN13. Black pepper seedlings treated with the promising bacteria had the lowest rate of root disease (8.45-11.21%) and lower fatal rate (11.11-15.55%) compared to the control group (24.81% and 24.44%). In addition, the three promising isolates strongly affected the growth of the black pepper seedlings in greenhouse. The plant height, length of roots, and fresh biomass of the seedlings in the treated plots were higher than those in the control plots. Thus, the endophytic bacterial isolates have the potential to act as biocontrol agent for the sustainable production of black pepper.

show abstract

Section: Introductionmentioning

confidence: 99%

Phytophthora Antagonism of Endophytic Bacteria Isolated from Roots of Black Pepper (Piper nigrum L.)

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…The effects of pH on enzyme activity and stability were analyzed, and showed in Figure 3 b and Figure 3 c. The pH activity profiles of the TKU034 chitosanase revealed maximum activity at pH 5 and pH 7 ( Figure 3 b). The optimum pH (pH 5) for TKU034 chitosanase activity was similar to that of most bacterial chitosanases, which display optimum activities at acidic pH values in a range from 4 to 7 [ 1 , 2 , 10 , 26 , 27 , 28 ]. The pH stability profiles of the TKU034 chitosanase were determined by measurement of the residual activity at pH 7 after incubation at various pH values at 37 °C for 60 min.…”

Section: Resultsmentioning

confidence: 88%

“…The molecular mass of the TKU034 chitosanase was approximately 43 kDa as confirmed by the SDS-PAGE ( Figure 2 ), which corresponded to the gel-filtration chromatography. The molecular mass of the TKU034 chitosanase (43 kDa) was similar to most chitosanases, which have a medium apparent molecular mass within the range of 20–75 kDa [ 13 , 26 ], but bacterial ones, especially Bacillus species, are usually between 40 and 50 kDa [ 1 , 2 , 26 , 27 , 28 , 29 , 30 ]. In our previous study, a microbial chitosanase was reported from B. cereus TKU033 using SPP as the sole carbon/nitrogen source, it showed also a molecular weight of around 43 kDa [ 31 ].…”

Section: Resultsmentioning

confidence: 99%

Chitinolytic Bacteria-Assisted Conversion of Squid Pen and Its Effect on Dyes and Pigments Adsorption

Liang

Wang

2015

Marine Drugs

Self Cite

View full text Add to dashboard Cite

The aim of this work was to produce chitosanase by fermenting from squid pen, and recover the fermented squid pen for dye removal by adsorption. One chitosanase induced from squid pen powder (SPP)-containing medium by Bacillus cereus TKU034 was purified in high purification fold (441) and high yield of activity recovery (51%) by ammonium sulfate precipitation and combined column chromatography. The SDS-PAGE results showed its molecular mass to be around 43 kDa. The TKU034 chitosanase used for the chitooligomers preparation was studied. The enzyme products revealed that the chitosanase could degrade chitosan with various degrees of polymerization, ranging from 3 to 9, as well as the chitosanase in an endolytic manner. Besides, the fermented SPP was recovered and displayed a better adsorption rate (up to 99.5%) for the disperse dyes (red, yellow, blue, and black) than the water-soluble food colorants, Allura Red AC (R40) and Tartrazine (Y4). The adsorbed R40 on the unfermented SPP and the fermented SPP was eluted by distilled water and 1 M NaOH to confirm the dye adsorption mechanism. The fermented SPP had a slightly higher adsorption capacity than the unfermented, and elution of the dye from the fermented SPP was easier than from the unfermented. The main dye adsorption mechanism of fermented SPP was physical adsorption, while the adsorption mechanism of unfermented SPP was chemical adsorption.

show abstract

“…The molecular mass of the TKU033 chitosanase was approximately 43 kDa as confirmed by the SDS-PAGE ( Figure 2 ), which corresponded to the gel-filtration chromatography. The molecular mass of the TKU033 chitosanase (43 kDa) was similar to most chitosanases, which have a medium apparent molecular mass within the range of 20–75 kDa [ 7 , 20 ].…”

Section: Resultsmentioning

confidence: 95%

“…Chitosanases are enzymes that catalyze the hydrolysis of the β-1,4 glycosidic bonds of chitosan. These enzymes have been found in abundance in a variety of bacteria [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 ]. Although chitosanases are efficient in producing chitosan [ 2 , 3 , 4 , 6 ], chitosan has been produced on an industrial scale mostly by the N -deacetylation of chitin using sodium hydroxide [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Squid Pen Chitin Chitooligomers as Food Colorants Absorbers

et al. 2015

Self Cite

View full text Add to dashboard Cite

One of the most promising applications of chitosanase is the conversion of chitinous biowaste into bioactive chitooligomers (COS). TKU033 chitosanase was induced from squid pen powder (SPP)-containing Bacillus cereus TKU033 medium and purified by ammonium sulfate precipitation and column chromatography. The enzyme was relatively more thermostable in the presence of the substrate and had an activity of 93% at 50 °C in a pH 5 buffer solution for 60 min. Furthermore, the enzyme used for the COS preparation was also studied. The enzyme products revealed various mixtures of COS that with different degrees of polymerization (DP), ranging from three to nine. In the culture medium, the fermented SPP was recovered, and it displayed a better adsorption rate (up to 96%) for the disperse dyes than the water-soluble food colorants, Allura Red AC (R40) and Tartrazne (Y4). Fourier transform-infrared spectroscopic (FT-IR) analysis proved that the adsorption of the dyes onto fermented SPP was a physical adsorption. Results also showed that fermented SPP was a favorable adsorber and could be employed as low-cost alternative for dye removal in wastewater treatment.

show abstract

Production, purification and characterisation of a chitosanase from Bacillus cereus

Cited by 24 publications

References 20 publications

Phytophthora Antagonism of Endophytic Bacteria Isolated from Roots of Black Pepper (Piper nigrum L.)

Phytophthora Antagonism of Endophytic Bacteria Isolated from Roots of Black Pepper (Piper nigrum L.)

Chitinolytic Bacteria-Assisted Conversion of Squid Pen and Its Effect on Dyes and Pigments Adsorption

Squid Pen Chitin Chitooligomers as Food Colorants Absorbers

Contact Info

Product

Resources

About