Production, purification, and characterization of an intracellular aflatoxin-detoxifizyme from Armillariella tabescens (E-20)

Liu, Daling; Yao, Dongsheng; Liang, Yu-Qiang; Zhou, Tianhong; Song, Yanping; Zhao, Long; Ma, Lin

doi:10.1016/s0278-6915(00)00161-7

Cited by 74 publications

(58 citation statements)

References 11 publications

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“…(Krifaton et al, 2011;Samuel et al, 2014;Sangare et al, 2014). Likewise, a number of fungal strains such as Armillariella tabescens (Liu et al, 2001), Phanerochaete sordida (Wang et al, 2011), Pleurotus ostreatus (Yehia, 2014) and a host of others (Adebo et al, 2015;Alberts et al, 2009;Wu et al, 2009) have been found to transform AFB 1 to less toxic products.…”

Section: Introductionmentioning

confidence: 99%

Aflatoxin B 1 degradation by liquid cultures and lysates of three bacterial strains

Adebo

Njobeh

Sidu³

et al. 2016

International Journal of Food Microbiology

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Section: Introductionmentioning

confidence: 99%

Aflatoxin B 1 degradation by liquid cultures and lysates of three bacterial strains

Adebo

Njobeh

Sidu³

et al. 2016

International Journal of Food Microbiology

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“…The aflatoxin B1 (AFB1) is one of the most extensive in distribution and has the most stable chemical properties (Liu et al 2001;Massey et al 1995). AFB1 is widely present in feeds and foods and cause the loss of nutrients and make the decline of nutritive value (Robens and Richard 1992).…”

Section: Introductionmentioning

confidence: 99%

The aflatoxin-detoxifizyme specific expression in mouse parotid gland

et al. 2015

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The aflatoxin-detoxifizyme (ADTZ) gene derived from Armillariella tabescens was cloned into parotid gland-specific expression vector (pPSPBGPneo) to construct the parotid gland-specific vector expressing ADTZ (pPSPBGPneo-ADTZ). Transgenic mice were generated by microinjection and identified by using PCR and Southern blotting analysis. PCR and Southern blotting analysis showed that total six transgenic mice carried the ADTZ gene were generated. RT-PCR analysis indicated that the expression of ADTZ mRNA could be detected only in parotid glands of the transgenic mice. The ADTZ activity in the saliva was found to be 3.72 ± 1.64 U/mL. After feeding a diet containing aflatoxin B1 (AFB1) for 14 days, the effect of ADTZ on serum biochemical indexes and AFB1 residues in serum and liver of mice were evaluated. The results showed that total protein and globulin contents in the test treatment (transgenic mice) produced ADTZ were significantly higher than that of the positive control, while alanine aminotransferase and aspartate aminotransferase activity in serum of the test treatment (transgenic mice) were remarkably lower compared to that of the positive control (P < 0.05). Moreover, AFB1 residues in serum and liver of the test treatment (transgenic mice) were significantly lower compared with that of the positive control (P < 0.05). These results in the study confirmed that ADTZ produced in transgenic mice could reduce, even eliminate the negative effects of AFB1 on mice.

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“…Liu et al (2001) isolated and characterised the enzyme responsible for aflatoxin degradation (the so-called aflatoxin-detoxifizyme) from Armillariella tabescens. This multienzyme complex is possibly responsible for opening the difuran ring of aflatoxin B 1 , thus leading to decreased mutagenic activity (Liu et al, 2001).…”

Section: Biological Methodsmentioning

confidence: 99%

Novel strategies to control mycotoxins in feeds: A review

Varga

Töth

2005

Acta Veterinaria Hungarica

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Mycotoxin-producing fungi may contaminate agricultural products in the field (preharvest spoilage), during storage (postharvest spoilage), or during processing. Mycotoxin contamination of foods and feeds poses serious health hazard to animals and humans. For lowering mycotoxin contamination of feeds and foods, several strategies have been investigated that can be divided into biological, chemical and physical methods. This paper gives an overview of strategies which are promising with regard to lowering the mycotoxin burden of animals and humans.

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Production, purification, and characterization of an intracellular aflatoxin-detoxifizyme from Armillariella tabescens (E-20)

Cited by 74 publications

References 11 publications

Aflatoxin B 1 degradation by liquid cultures and lysates of three bacterial strains

Aflatoxin B 1 degradation by liquid cultures and lysates of three bacterial strains

The aflatoxin-detoxifizyme specific expression in mouse parotid gland

Novel strategies to control mycotoxins in feeds: A review

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