Production, purification and characterization of two α-amylase isoforms from a newly isolated Aspergillus Oryzae strain S2

Sahnoun, Mouna; Béjar, Samír; Sayari, Aida Hmida; Triki, Mohamed Ali; Kriaa, Mouna; Kammoun, Radhouane

doi:10.1016/j.procbio.2011.09.016

Cited by 61 publications

(59 citation statements)

References 41 publications

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“…Optimum pH and temperature for xylanase, pectinase, mannanase and α-amylase of Aspergillus species were 4.0-5.5 and 50-65°C, respectively. These characteristics were similar to other previous report (Fang et al 2008;Sahnoun et al 2012;Sandri et al 2013;Squina et al 2009). Ang et al (2013) reported that the optimum pH of xylanase produced by A. fumigatus SK1 was pH 4.0.…”

Section: Glycosyl Enzyme Production Under Submerged Fermentationsupporting

confidence: 92%

Screening and Molecular Identification of New Microbial Strains for Production of Enzymes of Biotechnological Interest

Ghazala

Haddar

Romdhane

et al. 2016

Braz. arch. biol. technol.

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Section: Glycosyl Enzyme Production Under Submerged Fermentationsupporting

confidence: 92%

Screening and Molecular Identification of New Microbial Strains for Production of Enzymes of Biotechnological Interest

Ghazala

Haddar

Romdhane

et al. 2016

Braz. arch. biol. technol.

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“…Isolation and screening of fungi for glucose oxidase enzyme producing strain More than 75 different fungi strains were isolated from a variety of contaminated and decaying cereal samples. The samples were homogenized in sterile distilled water and microbes were isolated using spread plate and streaking methods on a Czapek medium [20]. The fungi were isolated from the plates and subcultured on potato dextrose agar (PDA).…”

Section: Methodsmentioning

confidence: 99%

Biocontrol of tomato plant diseases caused by Fusarium solani using a new isolated Aspergillus tubingensis CTM 507 glucose oxidase

Kriaa

Hammami

Sahnoun

et al. 2015

Comptes Rendus. Biologies

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The present study focuses on the potential of glucose oxidase (GOD) as a promising biocontrol agent for fungal plant pathogens. In fact, a new GOD producing fungus was isolated and identified as an Aspergillus tubingensis. GOD (125 AU) has been found to inhibit Fusarium solani growth and spore production. Indeed, GOD caused the reduction of spores, the formation of chlamydospores, the induction of mycelial cords and the vacuolization of mycelium. In vivo assays, GOD acted as a curative treatment capable of protecting the tomato plants against F. solani diseases. In fact, the incidence was null in the curative treatment with GOD and it is around 45% for the preventive treatment. The optimization of media composition and culture conditions led to a 2.6-fold enhancement in enzyme activity, reaching 81.48U/mL. This study has demonstrated that GOD is a potent antifungal agent that could be used as a new biofungicide to protect plants from diseases.

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“…The production of exoamylases has been described for the yeasts Aureobasidium pullulans (Li et al, 2007) and Schizosaccharomyces pombe (Okuyama et al, 2005). Furthermore, predominantly dextrinizing activity has been reported for several species of Aspergillus, such as A. flavus, A. niger and A. oryzae (Shafique et al, 2009;Sahnoun et al, 2012).…”

Section: Dextrinization and Saccharification Potential Of Enzymatic Ementioning

confidence: 99%

“…To meet the demand for this biofuel, several studies have focused on devising efficient mechanisms to hydrolyze starch and cellulose, thereby obtaining free glucose that can be fermented into ethanol by S. cerevisiae. In this regard, enzymes that catalyze the hydrolysis of these polymers are considered very important in the global energy scenario (Gupta et al, 2003;Sahnoun et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Bioprospecting of yeasts for amylase production in solid state fermentation and evaluation of the catalytic properties of enzymatic extracts

Oliveira¹,

Silvestre²,

Alves-Prado³

et al. 2015

Afr. J. Biotechnol.

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Profiling microorganisms with potential for amylase production in low cost culture media has been widely recognized due to its broad applicability. The aim of this study was to select yeast strains with potential to produce amylolytic enzymes by solid state fermentation. Fifty-four (54) strains were assessed and three exhibited ability to produce amylases: Candida parapsilosis with 14.68 U/mL (146.8 U/g substrate); Rhodotorula mucilaginosa with 25.0 U/mL (250 U/g substrate), and Candida glabrata with 25.39 U/mL (253.9 U/g substrate), in solid state fermentation, for 120 h at 28°C, using wheat bran with 70% moisture. The enzymes exhibited maximum activity at a pH of 7.0 and at 60°C. Amylases demonstrated satisfactory structural stability, maintaining their catalytic activity after 1 h at 50°C. All enzymes were ethanol tolerant and retained more than 70% of their original activities in 15% ethanol solution. Corn starch was efficiently hydrolyzed by enzymes and the extracts produced by C. parapsilosis and C. glabrata exhibited dextrinizing activity, while those produced by R. mucilaginosa exhibited saccharifying activity.

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Production, purification and characterization of two α-amylase isoforms from a newly isolated Aspergillus Oryzae strain S2

Cited by 61 publications

References 41 publications

Screening and Molecular Identification of New Microbial Strains for Production of Enzymes of Biotechnological Interest

Screening and Molecular Identification of New Microbial Strains for Production of Enzymes of Biotechnological Interest

Biocontrol of tomato plant diseases caused by Fusarium solani using a new isolated Aspergillus tubingensis CTM 507 glucose oxidase

Bioprospecting of yeasts for amylase production in solid state fermentation and evaluation of the catalytic properties of enzymatic extracts

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