Production, Purification and Characterization of an Antibacterial Compound From Streptomyces Griseus Tbg19nra1 Isolated From the Forest Soils of the Western Ghats of Kerala, India

Abstract

A new actinomycete strain designated TBG19NRA1, exhibiting antimicrobial activities against Gram-positive and Gram-negative bacteria, was isolated from forest soil of Neyyar Wild Life Sanctuary, Thiruvananthapuram, Kerala. Cultural characteristics strongly suggested that this isolate belongs to the genus Streptomyces. Polyphasic taxonomic studies including phylogenetic analysis based on 16S rDNA sequence suggests that the isolate is a strain of S. griseus. Studies on the effect of different nutritional compounds on antibiotic activity revealed that the highest antibacterial activity was obtained when 1% starch (w/v) was used as the sole carbon source along with mineral trace elements. Extraction and purification of TBG19NRA1 culture supernatant led to the isolation of a pure molecule with good antibacterial activity. The compound has been identified as the cyclic polyether lasalocid A on the basis IR, MS and 13C NMR data interpretation and comparison with reference data from literature.

Purification and Characterization of Bioactive Metabolite from Streptomyces monomycini RVE129 Derived from the Rift Valley Soil of Hawassa, Ethiopia

“…Further, the IR spectra of purified fraction RVE-02 are almost identical to functional groups of the polyketide antimicrobial metabolite as reported for setamycin by Omura et al [ 29 ], exhibiting peaks in the range of 3400–3500 cm −1 , around 1660 cm −1 , and 1717–1730 cm −1 which indicated hydroxyl, a hydrogen bonded carbonyl group and ketonic group or ester carbonyl, respectively. The chemical shift values of NMR 1 H and 13 C shown by the purified antibiotic RVE-02 were very similar to that of setamycin [ 39 ].…”