Production, Purification, and Properties of an Extracellular Laccase from Rigidoporus lignosus

Cambria, MariaTeresa; Cambria, Antonio; Ragusa, Santa; Rizzarelli, Enrico

doi:10.1006/prep.1999.1126

Cited by 60 publications

(38 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Also, a single laccase with a molecular mass of about 54 kDa has been reported. 8 In our study a single laccase from R. lignosus W1 with a molecular mass of about 40 kDa was found, similar to other low-molecular-mass (43 kDa) laccases reported. 19,24 These results illustrate the diversity of laccases, and even fungi belonging to the same species produce different laccases.…”

Section: Discussionsupporting

confidence: 87%

“…It has been widely reported that laccases have quite different catalytic activities under different incubation conditions. 8,11,17,19 The laccase from R. lignosus W1 exhibited favourable activity at acidic pH and had good stability above pH 4. Although no laccase activity was detected at alkaline pH, the laccase activity increased during storage at alkaline pH.…”

Section: Discussionmentioning

confidence: 99%

“…7 They catalyse the oxidation of a variety of phenolic and organic substrates coupled with the reduction of molecular oxygen to water. 8 Since laccases have wide substrate specificity, are secreted extracellularly and are tolerant to pollutants at high concentrations, they have attracted growing attention in many industrial and environmental fields. Recently, a number of studies have focused on the dye degradation capacities of fungal laccases and shown that these laccases are indeed major dye-decolorising enzymes in many white rot fungi.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Decolorisation of synthetic dyes by crude laccase from Rigidoporus lignosus W1

Dai

Peng

et al. 2008

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

BACKGROUND: Effluents from the dyeing process containing dyes are difficult to degrade biologically. Therefore enzymatic dye decolorisation has received considerable attention recently. In this study the dye decolorisation potential of crude laccase from the white rot fungus Rigidoporus lignosus W1 was demonstrated on an anthraquinone dye, Remazol Brilliant Blue R (RBBR), and a triphenylmethane dye, malachite green (MG). Effects of pH, temperature and ionic strength on laccase activity and decolorisation efficiency were investigated.

show abstract

Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Decolorisation of synthetic dyes by crude laccase from Rigidoporus lignosus W1

Dai

Peng

et al. 2008

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

show abstract

“…However, Lac II maintained 100% activity at 50°C even after 120 min. Some fungal laccases are thermostable, although the most white-rot fungi laccases are not active above 50°C (Cambria et al 2000, Das et al 2001, Hublick & Shinner 2000.…”

Section: Enzyme Characterizationmentioning

confidence: 98%

Properties of Laccases Produced by Pycnoporus sanguineus Induced by 2,5-xylidine

2006

View full text Add to dashboard Cite

Two isoforms of laccase produced from the culture supernatant of Pycnoporus sanguineus were partially purified by phenyl-Sepharose chromatography. Molecular masses of the enzymes were 80 kDa (Lac I) and 68 kDa (Lac II). Optimum activity of Lac I was at pH 4.8 and 30 degrees C, and Lac II was at pH 4.2 and 50 degrees C over 5 min reaction. The Km values of enzymes toward syringaldazine were 10 microM: (Lac I) and 8 microM: (Lac II). Sodium azide inhibited Lac I (85%) and Lac II (75%) activities.

show abstract

“…Epidemiological studies of H. brasiliensis natural forest and plantations showed high fungal density in soil (Nandris et al 1988) suggesting a capacity for high biodegradative ability of plant residues by R. microporus. Majority of previous studies on R. microporus has been directed at population biology and molecular phylogeny Oghenekaro et al 2014), pathogenicity (Farid et al 2009;Kaewchai et al 2009;Madushani et al 2013), host-parasite interactions (Nicole et al, 1985;Nicole et al 1986a;1986b), peroxidases (Geiger et al 1989) and laccases, with the latter being isolated, purified and studied in detail (Nicole et al 1992;Bonomo et al 1998;2001;Cambria et al, 2000;2011;2012). In vitro studies have focused on biological control of the pathogen Ogbebor et al 2015).…”

Section: Introductionmentioning

confidence: 99%

The saprotrophic wood-degrading abilities of <i>Rigidoporus microporus</i>

Oghenekaro¹,

Daniel²,

Asiegbu³

2015

Silva Fenn.

View full text Add to dashboard Cite

• Rigidoporus microporus isolates displayed varying saprotrophic capabilities on wood blocks of Rubber tree (Hevea brasiliensis).• Percentage mass loss of (Hevea brasiliensis) wood blocks caused by the pathogenic Rigidoporus microporus was significantly higher than that observed with the endophytic isolate.• The endophytic isolate has very poor saprotrophic ability on Hevea brasiliensis wood blocks. AbstractSaprotrophic wood-decaying abilities of Rigidoporus microporus (Polyporales, Basidiomycota) syn. Rigidoporus lignosus and the structural alterations induced in wood blocks of Hevea brasiliensis Muell. Arg were studied. Mass loss of wood blocks was analyzed after 3 and 6 months respectively and the patterns of decay by pathogenic and endophytic isolates of this fungus were investigated using light microscopy. Effects of temperature on growth of the isolates on malt extract agar were also investigated. The R. microporus isolated from a non-H. brasiliensis host caused the highest percentage mass loss (27.2% after 6 months), followed by isolates ED310 (21.1%) and M13 (15.7%), both collected from diseased H. brasiliensis plantations. The isolate initially identified as an endophyte showed very low saprotrophic wood decay capability (4.3% after 6 months). The optimal temperature for growth of the isolates was 30 °C; except for the endophytic isolate which showed highest growth at 25 °C. Wood samples degraded by the R. microporus isolates showed simultaneous attack of wood cell walls, typical of white rot fungi. Results of the study indicate variability in the wood degrading abilities of the isolates and the potential differences in their physiology are discussed. Our findings further support the need for a taxonomical revision of the Rigidoporus genus.

show abstract

Production, Purification, and Properties of an Extracellular Laccase from Rigidoporus lignosus

Cited by 60 publications

References 28 publications

Decolorisation of synthetic dyes by crude laccase from Rigidoporus lignosus W1

Decolorisation of synthetic dyes by crude laccase from Rigidoporus lignosus W1

Properties of Laccases Produced by Pycnoporus sanguineus Induced by 2,5-xylidine

The saprotrophic wood-degrading abilities of <i>Rigidoporus microporus</i>

Contact Info

Product

Resources

About