Productivity enhancement of S‐adenosylmethionine in Saccharomyces cerevisiae using n‐hexadecane as oxygen vector

Li, Minghua; Meng, Xiumei; Diao, Enjie; Du, Fang; Zhao, Xiaoyan

doi:10.1002/jctb.3752

Cited by 25 publications

(11 citation statements)

References 30 publications

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“…Furthermore, much more oleic acid remained in the medium at the end of the fermentation. A similar result was observed in S-adenosylmethionine fermentation by Saccharomyces cerevisiae using n-hexadecane as oxygen-vector, which may due to that the yeast cells and the oxygen transfer rate was negative affected by the high organic liquid content [14]. It was reported that the negative effect of excessive amount of oxygen-vectors on aerobic fermentation may be due to the increase in the apparent viscosity that reduces the oxygen transfer rate [9], and decline in the output of aerobic fermentation with an excessive amount of oxygen-vector concentration has been reported numerously [9,[28][29][30].…”

Section: Effect Of Oleic Acid Concentrationsupporting

confidence: 76%

Improved production of citric acid by Yarrowia lipolytica using oleic acid as the oxygen‐vector and co‐substrate

Liu

Xia

et al. 2016

Engineering in Life Sciences

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Improved production of citric acid by Yarrowia lipolytica using oleic acid as the oxygen-vector and co-substrate Yarrowia lipolytica is able to secrete large amounts of citric acid (CA), which is greatly affected by the dissolved oxygen concentration (DOC) in the fermentation medium. In this study, oleic acid was selected as oxygen-vector to improve DOC during CA fermentation. When 2% (v/v) of oleic acid was added to the culture broth, higher DOC (>42.1%) was determined throughout the CA synthesis phase. The yield of CA reached a maximum of 32.1 g/L (25.4% higher than the control) and the biomass was 8.8 g/L. The substrate uptake rate, products formation rate and key enzyme activities were also determined, and the results indicated that CA synthesis was strengthened with oleic acid addition. Furthermore, it was detected that oleic acid could be assimilated by the cells, which means that oleic acid could be served both as oxygen-vector and co-substrate for CA synthesis by Y. lipolytica. In a bioreactor with working volume of 3 L, the highest concentration of CA reached to 36. 4 g/L in the presence of 2% (v/v) oleic acid after 192 h of fermentation. These results confirmed that oleic acid could be applied in the large-scale production of CA by Y. lipolytica.

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Section: Effect Of Oleic Acid Concentrationsupporting

confidence: 76%

Improved production of citric acid by Yarrowia lipolytica using oleic acid as the oxygen‐vector and co‐substrate

Liu

Xia

et al. 2016

Engineering in Life Sciences

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“…Several studies of S. cerevisiae and other microorganisms such as Escherichia coli and Candida utilis supported the results of the metabolome analysis (Figs. 1 , 2 ) since further improvements of SAM production were attained by an elevation of intracellular ATP level by excess oxygen supply, gene knockdown, and optimization of carbon source feeding (Chen et al 2015 ; Li et al 2012 ; Wang et al 2013 ). It was reported that SAM accumulation in S. cerevisiae was increased by improving methionine and ATP availability by MET6 and SAM2 co-expression combined with sodium citrate feeding (Chen et al 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Metabolome analysis of Saccharomyces cerevisiae and optimization of culture medium for S-adenosyl-l-methionine production

2016

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S-Adenosyl-l-methionine (SAM) is a fine chemical used as a nutritional supplement and a prescription drug. It is industrially produced using Saccharomyces cerevisiae owing to its high SAM content. To investigate the optimization of culture medium components for higher SAM production, metabolome analysis was conducted to compare the intracellular metabolite concentrations between Kyokai no. 6 (high SAM-producing) and laboratory yeast S288C (control) under different SAM production conditions. Metabolome analysis and the result of principal component analysis showed that the rate-limiting step for SAM production was ATP supply and the levels of degradation products of adenosine nucleotides were higher in Kyokai 6 strain than in the S288C strain under the l-methionine supplemented condition. Analysis of ATP accumulation showed that the levels of intracellular ATP in the Kyokai 6 strain were also higher compared to those in the S288C strain. Furthermore, as expected from metabolome analysis, the SAM content of Kyokai 6 strain cultivated in the medium without yeast extract increased by 2.5-fold compared to that in the additional condition, by increasing intracellular ATP level with inhibited cell growth. These results suggest that high SAM production is attributed to the enhanced ATP supply with l-methionine condition and high efficiency of intracellular ATP consumption.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-016-0210-3) contains supplementary material, which is available to authorized users.

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“…As ATP is difficult to manipulate in the same simple way, optimization of the fermentation process is an alternative option to enhance ATP supply. 15,16 But beyond that, there is a lack of reliable and efficient methods for ATP regulation.…”

Section: Introductionmentioning

confidence: 99%

Control of ATP concentration in Escherichia coli using an ATP-sensing riboswitch for enhanced S-adenosylmethionine production

et al. 2017

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Productivity enhancement of S‐adenosylmethionine in Saccharomyces cerevisiae using n‐hexadecane as oxygen vector

Cited by 25 publications

References 30 publications

Improved production of citric acid by Yarrowia lipolytica using oleic acid as the oxygen‐vector and co‐substrate

Improved production of citric acid by Yarrowia lipolytica using oleic acid as the oxygen‐vector and co‐substrate

Metabolome analysis of Saccharomyces cerevisiae and optimization of culture medium for S-adenosyl-l-methionine production

Control of ATP concentration in Escherichia coli using an ATP-sensing riboswitch for enhanced S-adenosylmethionine production

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