Produksi Xilanase Menggunakan Media Limbah Pertanian Dan Perkebunan

Trismilah, Trismilah; Waltam, Deden Rosid

doi:10.29122/jtl.v10i2.1485

Cited by 5 publications

(3 citation statements)

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“…Limbah pertanian banyak mengandung lignoselulosa (hemiselulosa, selulosa, dan lignin) dan karbohidrat yang tinggi (Trismilah & Waltam 2009). Hemiselulosa merupakan heteropolimer polisakarida yang keberadaaannya paling melimpah kedua di alam setelah selulosa.…”

Section: Pendahuluanunclassified

Karakterisasi Xilanase dari Bakteri Xilanolitik XJ20 asal Tanah Hutan Taman Nasional Bukit Duabelas Jambi Indonesia

Mazidah¹,

Ambarsari²,

Meryandini³

2016

JSDH

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Xylanase is an extracellular enzymes that can be produced by microbes which capable to degrade xylan into xilo-oligosaccharides (XOS) and xylose. Application of xylanase is still not optimal due to the lack of availability of superior microbial cultures producing xylanase. The purpose of this study was to determine the characteristics of xylanase produced by bacteria isolated from Forest land Bukit Duabelas National Park, Jambi, Indonesia. Xylanase produced by bacterial isolate XJ20 was selected for further enzymatic activity and characteritics assays. Xylanolytic activity was measured by using the 3.5-dinitrosalicylic acid (DNS) assay. High activity of xylanase was recorded at three times of incubation, which were 6 (0.015 U/mL), 14 (0.012 U/mL) and 20 (0.007 U/mL). The first peak and the second peak reached the highest activity at a temperature of 70 °C, however, the first peak reached the highest activity at pH 4.0 and a second peak at pH 7.0. pH 4.0 xylanase were incubated at room temperature and 70 °C has a half time consecutive 9 hours and 6 hours, whereas at pH 7.0, has a half time consecutive 4 hours and 3 hours. Stability of enzyme better at pH 4.0 and at room temperature.

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Section: Pendahuluanunclassified

Karakterisasi Xilanase dari Bakteri Xilanolitik XJ20 asal Tanah Hutan Taman Nasional Bukit Duabelas Jambi Indonesia

Mazidah¹,

Ambarsari²,

Meryandini³

2016

JSDH

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“…Bacteria need xylan as a substrate in order to produce xylanase because the presence of xylan in the fermentation medium stimulates bacteria to secrete extracellular enzymes that can break down xylan into simple molecules as a carbon source. Because using pure xylan on a large basis costs too much, agricultural waste can be used as a medium for alternative carbon sources with lignocellulose as the primary component [10].…”

Section: Introductionmentioning

confidence: 99%

Specific Thermophilic Bacterial Xylanase Enzyme Activity Using Rice Straw as Substrate and Its Possibility as an Eco-friendly Fabric Bleach

Irdawati,

Hidayati,

Advinda

et al. 2023

Advances in Biological Sciences Research

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Extracellular enzyme xylanase has a wide range of industrial uses. The xylanase enzymes made by thermophilic bacteria can hydrolyze xylan into xylose and xylooligosaccharides. Rice straw, for example, is a natural carbon source that can be utilized to create xylan carbon sources. One of xylanase's uses in industry is as an environmentally friendly bleaching agent (biobleaching). This study's objectives were to ascertain the xylanase enzyme's specific activity at varied substrate concentrations and to evaluate the enzyme's potential impact on fabric brightness. This investigation is experimental. This is an experimental study using RAL that includes three replications and six treatments. The xylanase enzyme's contribution to the fabric's whitening will be evaluated using the kappa number. At a substrate concentration of, the xylanase enzyme's specific activity peaked 4%, or 0.419 U/mg protein, based on the results. The kappa number results show that the xylanase enzyme from agricultural waste substrates can reduce the kappa number (2.21) whereas the kappa number without xylanase is higher. This is supported by the potential activity of the xylanase enzyme on fabric brightness (3.42).

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“…This potential can be found in thermophilic microorganisms, namely microorganisms that live in high temperatures such as fungi and thermophilic bacteria [6] . Xylanase-producing bacteria were obtained from the genera Bacillus and Clostridium [7] and from Bacillus subtilis species [8] . SSA 2 isolates isolated from Sapan River Aro Solok Selatan is one of the thermophilic bacterial isolates capable of producing xylanase enzymes.…”

Section: Introductionmentioning

confidence: 99%

Potential of Xylanase Thermophilic Bacteria in the Pulp Biobleaching Process

Irdawati¹,

Putri²,

Syamsuardi³

et al. 2020

Proceedings of the International Conference on Biology, Sciences and Education (ICoBioSE 2019)

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Xylanase enzyme is an extracellular enzyme that has the ability to hydrolyze xylan to xylose. Xylanase can be produced by thermophilic bacteria and is a natural xylanase that is active and stable at high temperatures and alkaline pH, so it has the potential to be used in industrial and biotechnology fields. One industry that utilizes xylanase enzymes is the pulp and paper industry. At present, the pulp bleaching process carried out in Indonesia consists of a bleaching process using chlorine or hydrogen peroxide. The substitution of the use of chlorine with xylanase enzymes is a worthy alternative to reduce the impact caused by the use of chlorine in the pulp bleaching process. This study aims to determine the potential of thermophilic bacterial xylanase enzymes on the brightness of the pulp, and determine the brightness of the pulp at variations in temperature and pH on pulp fermentation. The pulp is fermented with temperature variations (50ºC, 55ºC, 60ºC, 65ºC, 70ºC, 75ºC, 80ºC) and pH (7; 7.5; 8; 8.5; 9; 9.5; 10). The optimum temperature and pH that produces the lowest kappa number and highest enzyme activity is at 60ºC and pH 8.5.

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Produksi Xilanase Menggunakan Media Limbah Pertanian Dan Perkebunan

Cited by 5 publications

References 0 publications

Karakterisasi Xilanase dari Bakteri Xilanolitik XJ20 asal Tanah Hutan Taman Nasional Bukit Duabelas Jambi Indonesia

Karakterisasi Xilanase dari Bakteri Xilanolitik XJ20 asal Tanah Hutan Taman Nasional Bukit Duabelas Jambi Indonesia

Specific Thermophilic Bacterial Xylanase Enzyme Activity Using Rice Straw as Substrate and Its Possibility as an Eco-friendly Fabric Bleach

Potential of Xylanase Thermophilic Bacteria in the Pulp Biobleaching Process

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