The sugar-energy sector is highlighted in Brazilian agribusiness; however, poor water distribution and soil acidity have limited the yield and quality of cane fields. Gypsum improves the physical and chemical properties of the soil, attenuating the effects of water shortage and acidity in the edaphic environment. The aim of this study was to determine the effect of gypsum doses on the nutritional status, photochemical efficiency and technological quality of sugarcane. The experiment was conducted under field conditions using a randomized block design in a 3 x 5 factorial scheme with four replications. The treatments consisted of the combination of three sugarcane varieties (RB011941, RB92579, and RB991536) with five doses of gypsum (0 Mg ha-1 ; 2.5 Mg ha-1 ; 5.0 Mg ha-1 ; 10 Mg ha-1 ; and 20.0 Mg ha-1). The results showed that gypsum did not influence the evaluated variables. A varietal effect for leaf contents of P, K, Mg and S was observed. The RB92579 presented the highest levels of P (1.65 g kg-1) and K (14.55 g kg-1), while RB011941 showed the highest concentrations of Mg and S, 1.25 and 1.05 g kg-1 , respectively. The mean values of the macronutrients presented the following concentration order: N > K > Ca > Mg > P > S. Genotypes were similar in terms of photochemical efficiency, which in turn, was not influenced by gypsum application. The best technological quality was verified in RB92579, which showed the highest Brix percentage (22.35%), Pol (19.75%), and the highest levels of TRS (151.3 kg t-1) of sugarcane.