Proficiency testing to detect Trichinella larvae in meat in the European Union

Marucci, Gianluca; Tonanzi, Daniele; Cherchi, Simona; Galati, Fabio; Bella, Antonino; Interisano, Maria; Ludovisi, Alessandra; Amati, A.; Pozio, Edoardo

doi:10.1016/j.vetpar.2016.04.009

Cited by 17 publications

(33 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For these animals, the only way to avoid the occurrence of infections in humans and the spread of these parasites to other pigs and to wildlife is the systematic control using artificial digestion tests at the slaughterhouse for all pigs originating from uncontrolled housing (Gottstein et al, 2009). This test must be performed by trained analysts who participate in proficiency testing at least one per year (Marucci et al, 2016). To detect Trichinella infection in domestic or wild animals, samples of preferential muscles should be tested by artificial digestion (Nöckler and Kapel, 2007).…”

Section: Prevention and Controlmentioning

confidence: 99%

Trichinella and trichinellosis in Europe

Pozio

2019

Vet glas

View full text Add to dashboard Cite

Background: Trichinellosis, the proper term for the human zoonotic disease is caused by nematodes of the genus Trichinella. These zoonotic parasites show a cosmopolitan distribution in all the continents but Antarctica. They circulate in nature by synanthropicdomestic and sylvatic cycles that are correlated with each other. Today, nine species and three genotypes are recognized in this genus, all of which infect mammals, including humans, while one species also infects birds, and two other species also infect reptiles. Scope and Approach: To review the recent literature on these pathogens, which are unusual among the other nematodes in that the worm undergoes a complete developmental cycle, from larva to adult to larva, in the body of a single host, which has a profound influence on the epidemiology of trichinellosis as a zoonosis. When the cycle is complete, the muscles of the infected animal contain a reservoir of larvae capable of long-term survival. Humans and other hosts become infected by ingesting muscle tissues containing viable larvae.

show abstract

Section: Prevention and Controlmentioning

confidence: 99%

Trichinella and trichinellosis in Europe

Pozio

2019

Vet glas

View full text Add to dashboard Cite

show abstract

“…The overall success for all participants of 100% is equivalent to the best achieved (100% in 2012, 2014 and 2017) among EU laboratories in PTs organized by EURLP, 2007-2017. The percentage of National Reference Laboratories for Parasites (NRLPs) in EU member countries which passed PTs during 2007-2017 was between 83.3% and 100% (Marucci et al, 2016; data from EURLP web site). An exception occurred in 2009, when three samples of 100 g, each spiked with one larva, were included in the PT panel, and only 18 of 26 (69.2%) of participant laboratories correctly identified these samples.…”

Section: Resultsmentioning

confidence: 99%

“…The EQA panel consisted of three samples with identical numbers of larvae for all participants. Preparation of the three samples was described by Marucci et al (2016). Briefly, test material consisted of three 100±5 g minced pork meat balls.…”

Section: Methodsmentioning

confidence: 99%

“…It should be conducted under the direction of the National Reference Laboratory for Trichinella in each Member State of the EU, with the aim to ensure the quality of MSM and to evaluate the competence and performance of laboratories in Trichinella detection. In different EU member states, PTs have already been performed for more than 10 years (Marucci et al, 2009;Riehn et al, 2013;Marucci et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

First Serbian external quality assessment to detect Trichinella larvae in meat by the Magnetic Stirrer Method

Vasilev

Ilić

Sofronić-Milosavljević

2019

VET GLASNIK

View full text Add to dashboard Cite

Introduction. Inter-laboratory comparative testing must be conducted under the direction of the National Reference Laboratory for Trichinella in each Member State of the EU with the aim to ensure the quality of the approved digestion method and to evaluate the competence of laboratories in Trichinella detection. For the first time in Serbia, the National Reference Laboratory for Trichinellosis (NRLT INEP) organized such external quality assessment (EQA) for the detection of Trichinella larvae in meat by the Magnetic Stirrer Method (MSM). The aim of this study was to enable laboratories interested to take part in the EQA and to assess the quality of their MSM performance. Materials and Methods. The EQA was organized by NRLT INEP according to ISO/IEC 17043:2010. The EQA test panel, which was sent to eight laboratories within Veterinary Institutes, consisted of three 100±5 g minced pork meat balls with identical numbers of larvae. Two meat balls were spiked with five viable Trichinella spiralis muscle larvae (L1), and one meat ball was not spiked. Results and Conclusions. Evaluation made on the basis of qualitative results showed that 100% of participants successfully completed the testing. Quantitative evaluation showed that, on average, only 60% of Trichinella larvae were detected among all participants. The main importance of this study is that it enabled laboratories across the country to be compared. This should be efficient stimulus for improvement of analytical performance over time. Therefore, it will be of value if the participants and other official laboratories in Serbia take part annually in EQAs organized at national level. The results obtained in this study could provide useful information to the Ministry of Agriculture, Forestry and Water Management, Veterinary Directorate and be of importance for promoting the one health concept in the country as a whole.

show abstract

“…The slaughtered pigs from the test zone were analyzed in two groups, one encompassing pigs slaughtered in households and the other covering pigs slaughtered in industrial slaughterhouses. Trichinelloscopic examination was also conducted in two ways: trichinelloscopy and digestive testing of aggregate samples (Teodorović et al, 2000;Mirilović, 2006;Marucci et al, 2016).…”

Section: Methodsmentioning

confidence: 99%

Economic evaluation of a program for monitoring and controlling Trichinella in pigs

et al. 2019

View full text Add to dashboard Cite

Introduction. Trichinellosis is a cosmopolitan anthropozoonosis, and is a group of systemic diseases caused by larval forms of Trichinella. This is one of the oldest and most controversial parasitic zoonoses that has been in the forefront of veterinary, medical and biological research for many years. Parasites from the genus Trichinella were diagnosed in more than one hundred species of domestic and wild mammals and birds. The aim of this study was economic evaluation of a program for monitoring and controlling Trichinella in pigs. Materials and Methods. A systematic pest rodent control program deemed suitable for monitoring and controlling Trichinella in pigs was implemented in a representative test zone. The results of the pest rodent control program were analyzed using cost-benefit analysis to determine if a similar program would be suitable for a larger land area. Results and Conclusions. Prior to pest control, the incidence of Trichinella in pigs living in the test zone increased annually, while after systematic pest rodent control, the incidence of MIRILOVIĆ Milorad et al.: Economic evaluation of a program for Trichinella in pigs 117 Trichinella-positive pigs decreased. These results, as well as relevant economic indicators, enabled us to design two mathematical models describing the control and monitoring of Trichinella in a larger land area. In this area, Srem Region, Serbia, a suitable Trichinella control program in pigs is clearly justified because the profit to cost ratio was positive for both models.

show abstract

Proficiency testing to detect Trichinella larvae in meat in the European Union

Cited by 17 publications

References 6 publications

Trichinella and trichinellosis in Europe

Trichinella and trichinellosis in Europe

First Serbian external quality assessment to detect Trichinella larvae in meat by the Magnetic Stirrer Method

Economic evaluation of a program for monitoring and controlling Trichinella in pigs

Contact Info

Product

Resources

About