Profile of apoptotic proteins in oral squamous cell carcinoma: A cluster analysis of 171 cases

Coutinho‐Camillo, Cláudia Malheiros; Lourenço, Sílvia Vanessa; Puga, Renato; Damascena, Aline Santos; Teshima, Tathyane Harumi Nakajima; Kowalski, Luiz Paulo; Soares, Fernando Augusto

doi:10.1186/s41241-016-0008-2

Cited by 13 publications

(12 citation statements)

References 39 publications

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“…12 The expression of Bax and Bcl-2 has previously been ad with induction and repression of apoptosis, respectively, in oral cancer cell lines. 13,14 The results of this study demonstrate that expression of Bax and Bcl-2 is key to the induction of apoptosis in HSC-3 cells. Any alteration in the expression, especially overexpression of Bcl-2, has been found in histological studies of epithelial dysplasia, in early carcinogenesis of oral cancer.…”

Section: Discussionmentioning

confidence: 66%

Gene expression of selected apoptotic markers in human oral squamous carcinoma HSC-3 cell line treated with Myrmecodia pendans plant extract

Lestari¹,

Yusry²,

Iskandar³

et al. 2019

msk

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Background: Myrmecodia pendans (M. pendans), or Sarang Semut, is an epiphyte with anticancer potential. It was recently reported that it induces apoptotic activity in the human oral squamous carcinoma (HSC-3) cell line. This study aimed to investigate the effect of M. pendans treated samples on the expression of apoptotic markers, Bax and Bcl-2. Methods: M. pendans was purchased from West Papua, Indonesia. The hypocotyl was dried thoroughly and then extracted aqueously. The apoptotic activity was detected via flow cytometry. Bax and Bcl-2 expression was analyzed by quantitative real-time polymerase chain reaction. Results: Results of our cell cycle analysis reveal that aqueous extract of M. pendans induced apoptosis in 2.5 and 5 mg/mL but no change between these two concentrations. Apoptosis was observed at 24 h but not at 48 h. Bax and Bcl-2 expression in HSC-3 cells was affected by M. pendans. At 24 h, upregulation of Bax was observed at 2.5 mg/mL. However, after 48 h, Bax expression showed no changes at any concentration. Bcl-2 was significantly downregulated after 48 h of treatment. Conclusions: M. pendans extract induced apoptosis in HSC-3 cells, which might occur via the proapoptotic (Bax) and antiapoptotic (Bcl-2) pathways.

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Section: Discussionmentioning

confidence: 66%

Gene expression of selected apoptotic markers in human oral squamous carcinoma HSC-3 cell line treated with Myrmecodia pendans plant extract

Lestari¹,

Yusry²,

Iskandar³

et al. 2019

msk

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“…In addition, we found lower proliferation score, higher apoptosis score, higher metastasis-inhibiting score, and higher metastasis-promoting score in VEGF-high score subtype. Coutinho-Camillo et al defined a pro-apoptotic cluster and an anti-apoptotic cluster, indicating a correlation between apoptosis and tumor behavior [ 35 ]. Genes in metastasis-promoting signature were mostly related to the function of Neutrophil cell, Treg cell, and macrophage; genes in metastasis-inhibiting signature were mostly related to interferon regulatory family and the function of T cell, nature killer cell.…”

Section: Discussionmentioning

confidence: 99%

Correlation between vascular endothelial growth factor pathway and immune microenvironment in head and neck squamous cell carcinoma

Zhu

Liu

et al. 2021

BMC Cancer

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Background Immunotherapy is a crucial therapeutic approach in oncology. However, most patients with head and neck squamous cell carcinoma (HNSCC) do not derive benefit from immunotherapy. Vascular endothelial growth factor (VEGF)/VEGF Receptor 2 (VEGFR2) signaling pathway is one of the most important pathways regulating angiogenesis in tumor. The combination of immunotherapy and anti-angiogenic therapy is considered to improve efficacy of immunotherapy. The correlation between VEGF signaling pathway and tumor immune microenvironment in HNSCC patients is unclear. Methods We utilized RNA sequencing and clinical data of HNSCC patients from the TCGA database to study the correlation between VEGF signaling pathway and tumor immune microenvironment, on aspect of immune cell infiltration, immune-related gene expression profiling and immune-related biological pathways. Results We observed that VEGF signaling pathway is positively correlated with immune cell infiltration, immune-related gene expression profiles, and the prognosis of HNSCC patients. The functional enrichment analysis of differentially expressed genes between different VEGF score subtypes detected multiple immune-related biological processes. Conclusion Our findings suggested that combining anti-VEGF signaling pathway agents with immunotherapy, such as immune checkpoint inhibitors (ICI) therapy, may exhibit encouraging benefits in HNSCC.

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“…The cytochrome-c which is released into the cytosol binds to Apaf-1 and activates procaspase-9 in apoptosome. Caspase-9 activation causes activation of the caspase executor, in this case, caspase-3 will fragment the target protein including poly (ADP-ribose) polymerase resulting in cell death and cease of proliferation [19].…”

Section: Discussionmentioning

confidence: 99%

Expression of Ki67 in Precancerous Squamous Cell Skin Lesion of Mice Induced by DMBA

Nurkasanah¹,

Hoemardani

Sinuraya

2019

Indonesian Journal of Cancer

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Background: Squamous cell carcinoma (SCC) is an invasive skin cancer that is found in the human skin. SCC growth from the precancerous lesion is known as a wart or papilloma. Ki67 is a protein expressed by cells during proliferation. Cells with higher Ki67 expression showed abnormal regulation of apoptosis. 5-FU is an antimetabolite compound that works as a DNA/RNA pyrimidine antagonist molecule that can induce cell apoptosis. The main objective of this research is to evaluate the inhibition proliferation of precancerous squamous cell in skin lesion induced by DMBA/croton oil treated by using 5-FU cream topically compared to 5% imiquimod cream. Methods: This research assesses three different concentrations of 5-FU cream namely 1%, 2%, and 5% topically on 24 wild type mice divided into 6 groups of 4 each including positive control (with carcinogenesis but without treatment), negative control (without treatment ; normal), carcinogenesis with treatment 5-FU cream (1%, 2%, and 5%) or 5% imiquimod cream. The expression of Ki67 was analyzed using immunohistochemistry. Statistical analysis was performed by one-way ANOVA using SPSS version 23. Results: Based on this research, 5-FU cream treatment during 4 weeks (week 10-week 14) showed the decrease of cumulative number of papilloma (p<0.05) and immunohistochemistry analysis resulted in the lowest Ki67 expression in 5-FU 2% and 5% groups compared to 5% imiquimod cream (p<0.05). Conclusions: 5-FU cream treatment during 4 weeks showed a decrease of papilloma and Ki67 expression in 5-FU cream 2% and

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Profile of apoptotic proteins in oral squamous cell carcinoma: A cluster analysis of 171 cases

Cited by 13 publications

References 39 publications

Gene expression of selected apoptotic markers in human oral squamous carcinoma HSC-3 cell line treated with Myrmecodia pendans plant extract

Gene expression of selected apoptotic markers in human oral squamous carcinoma HSC-3 cell line treated with Myrmecodia pendans plant extract

Correlation between vascular endothelial growth factor pathway and immune microenvironment in head and neck squamous cell carcinoma

Expression of Ki67 in Precancerous Squamous Cell Skin Lesion of Mice Induced by DMBA

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