Profile of polyamines during sprouting and growth of saffron (Crocus sativus L.) Corms

Jirage, Dayadevi B.; Ravishankar, G. A.; Suvarnalatha, G.; Venkataraman, L. V.

doi:10.1007/bf00210949

Cited by 14 publications

(10 citation statements)

References 15 publications

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“…The regeneration rate of daughter corms under natural conditions is low, resulting in a limited production of propagating material for field cultivation (Jirage et al 1994;Chahota et al 2003). In vitro micropropagation can be used to produce large quantities of the propagating material in saffron (Majourhat et al 2007).…”

Section: Introductionmentioning

confidence: 98%

Study of the antioxidant enzymatic system during shoot development from cultured intercalar meristems of saffron

et al. 2011

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As preliminary research to develop a clonal in vitro propagation system for saffron, we have evaluated the morphogenic response of intercalary meristems present in shoots from sprouting saffron corms when cultured in media with different cytokinins. The best results in terms of shoot production and quality were achieved in the medium supplemented with 5 mg/l of benzylaminopurine (BAP). During the multiplication of the meristems, several changes in the activities of the antioxidant enzymatic system were detected. Catalase (CAT) activity was reduced in the presence of the assayed cytokinins, whereas peroxidase (POX) activity was stimulated with all the cytokinins assayed. When TDZ (thidizuron) was used, a slight decrease in superoxide dismutase (SOD) activity occurred, whereas BAP and 2iP (2-isopentenyl adenine) treatments produced a significant increase in SOD activity. The ascorbate-glutathione cycle enzymes showed a similar pattern in response to the addition of cytokinins. These results suggest a possible use of antioxidant enzyme activities as a parameter that could be use to evaluate the efficiency of micropropagation protocols for saffron and even in other species.

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Section: Introductionmentioning

confidence: 98%

Study of the antioxidant enzymatic system during shoot development from cultured intercalar meristems of saffron

et al. 2011

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“…Saffron being triploid (2n=3x=24) is sterile and is propagated vegetatively through daughter corms. Rate of generation of daughter corms under natural conditions is low (Jirage et al 1994, Chahota et al 2003. Low rate of daughter corm production results in limited availability of propagating material for field cultivation.…”

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confidence: 98%

In vitro cormlet development in Crocus sativus

Sharma¹,

Rathour²,

Sharma³

et al. 2008

Biologia plant.

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An improved protocol for generation of viable cormlets from tissue culture derived shoots of saffron has been developed. Multiple shoots were generated from apical buds, small corms and in vitro developed single shoots. Bunches of two to three shoots when cultured on half strength Murashige and Skoog (MS) medium containing 3 mg dm -3 benzyladenine (BA) and 80 g dm -3 sucrose developed 1.89 cormlets per shoot bunch with an average fresh mass of 1.18 g. It took nine months from culture of apical buds to the harvest of cormlets but under field conditions 22 months. Sucrose appeared to be essential for cormlet induction as no cormlets were developed in the medium devoid of sucrose and only 0.29 per shoot in medium containing mannitol. In vitro derived cormlets sprouted from apical and axillary buds on MS medium containing 12 mg dm -3 BA, 3 mg dm -3 indolebutyric acid and 30 g dm -3 sucrose. Daughter cormlet formation from in vitro derived cormlets was also observed.

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“…This spice is made from the dried stigmas of Crocus sativus L., a triploid sterile plant that is vegetatively propagated by means of corms. However, little is known about the physiology of the plant, especially of the corm, as many researches have focused their efforts on its biochemical composition (Chrungo et al, 1983;Farooq et al, 1985;Oda and Tatsumi, 1993;Jirage et al, 1994). The corm is a subterraneous stem that accumulates reserves for sprouting after dormancy periods.…”

Section: Introductionmentioning

confidence: 99%

Development and Gene Expression in Saffron Corms

Álvarez‐Ortí¹,

Gómez²,

Rubio³

et al. 2004

Acta Hortic.

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The corm occupies a central position in saffron life cycle, as it is the vegetative organ that accumulates reserves for sprouting after dormancy periods. Until now, not much is known about the physiology of the corm and its gene expression pattern. In an attempt to obtain information about this organ, six developmental stages were defined in the corm and analysed two of them, generating 650 partial complementary DNA sequences (expressed sequence tags, ESTs) from two cDNA libraries constructed from corms. In the first one, at a stage characterized by storage accumulation and corm growth (C3), a remarkable amount of sequences with similarity to genes related to cell growth; protein synthesis, folding and degradation; transcription factors; and proteins related to the formation and maintenance of cell wall and other cellular structures were found. Furthermore, many sequences with similarity to genes involved in defence and stress responses, and to carbohydrate metabolism were detected. In the second library, from dormant corms (C4), a greater percentage of sequences with similarity to genes related to transport of nutrients and metabolites was present. In both libraries, a high percentage of sequences with no similarity in the public databases was found (36 % in C3 and 50 % in C4) remarking the lack of knowledge about gene expression in the corm.

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Profile of polyamines during sprouting and growth of saffron (Crocus sativus L.) Corms

Cited by 14 publications

References 15 publications

Study of the antioxidant enzymatic system during shoot development from cultured intercalar meristems of saffron

Study of the antioxidant enzymatic system during shoot development from cultured intercalar meristems of saffron

In vitro cormlet development in Crocus sativus

Development and Gene Expression in Saffron Corms

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