In vitro cormlet development in Crocus sativus

Sharma, Kamal Dev; Rathour, Rajeev; Sharma, Rajesh; Goel, Shubhra; Sharma, T. R.; Singh, B. M.

doi:10.1007/s10535-008-0136-y

Cited by 38 publications

(28 citation statements)

References 14 publications

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“…In addition to IBA, sucrose level was increased to 5% in the growth media to induce root formation and to increase corm number. A study [10] which was published after the termination of our experiments in 2007 on Crocus sativus [31] also emphasized the importance of elevated sucrose concentration for corm production. The results of the present study show that IBA and increased sucrose level are critical factors to initiate adventitious root formation and their further growth.…”

Section: Discussionmentioning

confidence: 98%

“…Plant tissue culture techniques might offer a great potential for saffron production when compared to the traditional cultivation methods. In vitro propagation of saffron through direct and indirect organogenesis from callus cultures was investigated previously [7][8][9][10]. These studies tested the effects of factors such as temperature and phytohormones on the reproductive capacities of saffron, adventitious root, adventitious shoot and corm development.…”

Section: Introductionmentioning

confidence: 99%

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Improved in vitro micropropagation method with adventitious corms and roots for endangered saffron

2011

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The objective of this study was to investigate development of an efficient in vitro tissue culture system for saffron (Crocus sativus L.) complete with roots and corms. In indirect organogenesis, Murashige and Skoog (MS) media with 3% (w/v) sucrose, 100 mg L−1 ascorbic acid, and the combination of 0.25 mg L−1 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 mg L−1 6-benzylaminopurine (BAP) were best for callus initiation and growth while 1.5 mg L−1 BAP was excellent for high rate of adventitious shoot formation. 1 mg L−1 indole-3-butyric acid (IBA) was more preferable for adventitious corm and root initiation as well as growth. Overall, 64% rooting and 33% corm production rates were achieved in indirect organogenesis. In direct organogenesis, MS medium supplemented with 3 % sucrose, 100 mg L−1 ascorbic acid and 1 mg L−1 BAP was optimum for shoot growth. While 1 mg L−1 IBA was best for adventitious corm formation, 2 mg L−1 IBA promoted adventitious root initiation and growth. Overall, 36% and 57% of explants had corm and contractile root, respectively. The high rates suggest that efficient tissue culture system could be achieved for mass propagation and ex situ conservation of threatened saffron genetic resources.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Improved in vitro micropropagation method with adventitious corms and roots for endangered saffron

2011

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“…Ilahi et al (1987) reported that callus could be differentiated into shoots on the medium containing both NAA and BAP as also suggested by (Yildirim 2007). Similarly, the effects of different sugar concentrations on callus induction and shoot regeneration have also been reported (Sharma et al 2008) therefore deemed future investigations. This study demonstrated a large number of disease free high quality plantlets could be produced in a short period of time.…”

Section: Resultsmentioning

confidence: 85%

Factors Affecting Callus Induction and Organogenesis in Saffron (Crocus sativus L.)

Vahedi

Kalantari

Salami

2014

Plant Tissue Cult. & Biotech.

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Saffron (Crocus sativus L.) belongs to Iridaceae and is known an important native commercial plants in Iran for its high value of saffron. The best growth regulator composition for callus production from corms and sahoot regeneration from callus were determined. Saffron corms harvested from previous crops are generally used for future cropping cycles. However, this practice causes major yield losses due to the heavy attack by different pathogens. Availability of healthy disease free planting materials is of great importance for successful cultivation of saffron. By this investigation the best composition of growth regulators for callus production from corms and shoot regeneration from callus were determined. Callus induction of Crocus sativus L. was investigated by using different combinations NAA, 2, 4-D and TDZ, BA and Kn. The highest frequency of callus induction was observed in medium containing 2 mg/l 2, 4-D + 1 mg/l BA followed by 1 mg/l 2, 4-D + 0.15 mg/l Kn. However, in case of growth parameters such as diameter and the area of calli the best result was obtained in the medium supplemented with 2 mg/l 2, 4-D + 1 mg/l BA. In some treatments, calli were transferred to organogenesis stage after two subcultures. For sprouting of shoots transferred to medium containing 1 mg/l BAP and 1 mg/l NAA. Statistical analyses indicated that the treatment containing 5 mg/l NAA and 5 mg/l TDZ proved to be the best growth regulator treatment for shoot regeneration from the saffron calli.

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“…Shoot formation was observed from ovaries and apical buds. In vitro development of microcorms from apical buds, corms, meristems and ovaries has been obtained by other researchers (Sharma et al 2008;Mir et al 2010). Microcorm formation from apical bud takes only 8 months under in vitro conditions as against 22 months under field conditions.…”

Section: Resultsmentioning

confidence: 87%

“…Our results showed further improvement over the protocols developed earlier (Ding et al 1979;Ding et al 1981), who first reported tissue culture of C. sativus using MS medium containing IAA and/or 2, 4-D. Development of microcorms on the surface of explants (1/8 corm fragments) (Homes et al 1987) and differentiation of corms into buds (Ilahi et al 1987) has been reported. In vitro microcorm production has been obtained by culturing leaf segments and apical buds of saffron (Raja et al 2007;Sharma et al 2008). Shoot formation was observed from ovaries and apical buds.…”

Section: Resultsmentioning

confidence: 99%

In vitro development of microcorms and stigma like structures in saffron (Crocus sativus L.)

Mir

Ahmed

Wani

et al. 2010

Physiol Mol Biol Plants

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Saffron is an important spice derived from the stigmas of Crocus sativus, a species belonging to the family Iridaceae. Due to its triploid nature it is sterile and is not able to set seeds, so it is propagated only by corms. The natural propagation rate of most geophytes including saffron is relatively low. An in vitro multiplication technique like micropropagation has been used for the propagation of saffron. In the present study, various explants were cultured on different nutrient media supplemented with various concentrations of plant growth regulators to standardize the best media combination for obtaining optimum response with respect to corm production and development of Stigma Like Structures (SLS). Highest response (60%) was observed with half ovaries on G-5 media supplemented with 27μM NAA and 44.4μM BA followed by 55% on LS media with 27μM NAA and 44.4μM BA. Maximum size (1.3 g) of microcorms were obtained from apical buds on the LS media supplemented with 21.6μM NAA and 22.2μM. Stigma Like Structures were developed from half ovary explants both directly and indirectly. Maximum number (120 indirectly and 20 directly) and size (5.2 cm) of SLS were obtained in G-5 medium supplemented with 27μM NAA and 44.4μM BA followed by 100 indirectly and 20 directly and 4.5 cm long on LS medium supplemented with 27μM NAA and 44.4μM BA.

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In vitro cormlet development in Crocus sativus

Cited by 38 publications

References 14 publications

Improved in vitro micropropagation method with adventitious corms and roots for endangered saffron

Improved in vitro micropropagation method with adventitious corms and roots for endangered saffron

Factors Affecting Callus Induction and Organogenesis in Saffron (Crocus sativus L.)

In vitro development of microcorms and stigma like structures in saffron (Crocus sativus L.)

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