Profile of protein lysine propionylation in Aeromonas hydrophila and its role in enzymatic regulation

Miao, Yanying; Wang, Yuqian; Huang, Dongping; Lin, Xiaoke; Lin, Zhenping; Lin, Xiangmin

doi:10.1016/j.bbrc.2021.05.050

Cited by 2 publications

(2 citation statements)

References 32 publications

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“…Meanwhile, it was revealed that propionyl-CoA carboxylase deficiency in Caenorhabditis elegans resulted in reduced lifespan through disordered energy metabolism, comprising mitochondrial OXPHOS capacity reduction, oxidative stress increase, and distal tricarboxylic acid (TCA) cycle flux inhibition [ 28 ]. In line with these findings, lysine propionylation was also found to mediate energy metabolism in Aeromonas hydrophila , while site-specific propionylation modifications negatively regulate malate dehydrogenase metabolic activity [ 29 ]. Thus, propionylation plays a crucial role in modulating gene expression, maintaining protein stability, and participating in energy metabolism.…”

Section: The Identification and Characterization Of Novel Acylationmentioning

confidence: 82%

A glimpse into novel acylations and their emerging role in regulating cancer metastasis

Shi,

Cui,

Qin

et al. 2024

Cell. Mol. Life Sci.

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Metastatic cancer is a major cause of cancer-related mortality; however, the complex regulation process remains to be further elucidated. A large amount of preliminary investigations focus on the role of epigenetic mechanisms in cancer metastasis. Notably, the posttranslational modifications were found to be critically involved in malignancy, thus attracting considerable attention. Beyond acetylation, novel forms of acylation have been recently identified following advances in mass spectrometry, proteomics technologies, and bioinformatics, such as propionylation, butyrylation, malonylation, succinylation, crotonylation, 2-hydroxyisobutyrylation, lactylation, among others. These novel acylations play pivotal roles in regulating different aspects of energy mechanism and mediating signal transduction by covalently modifying histone or nonhistone proteins. Furthermore, these acylations and their modifying enzymes show promise regarding the diagnosis and treatment of tumors, especially tumor metastasis. Here, we comprehensively review the identification and characterization of 11 novel acylations, and the corresponding modifying enzymes, highlighting their significance for tumor metastasis. We also focus on their potential application as clinical therapeutic targets and diagnostic predictors, discussing the current obstacles and future research prospects.

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Section: The Identification and Characterization Of Novel Acylationmentioning

confidence: 82%

A glimpse into novel acylations and their emerging role in regulating cancer metastasis

Shi,

Cui,

Qin

et al. 2024

Cell. Mol. Life Sci.

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“…Lysine propionylation has been found in histones and a number of bacterial proteins. − The direct incorporation of propionyl-lysine into recombinant histones has been established in E. coli cells by WT Mm PylRS or an Mb PylRS variant (Y271F and C313T) …”

Section: Lysine Modificationsmentioning

confidence: 99%

Orthogonal Translation for Site-Specific Installation of Post-translational Modifications

Gan,

Fan

2024

Chem. Rev.

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Post-translational modifications (PTMs) endow proteins with new properties to respond to environmental changes or growth needs. With the development of advanced proteomics techniques, hundreds of distinct types of PTMs have been observed in a wide range of proteins from bacteria, archaea, and eukarya. To identify the roles of these PTMs, scientists have applied various approaches. However, high dynamics, low stoichiometry, and crosstalk between PTMs make it almost impossible to obtain homogeneously modified proteins for characterization of the site-specific effect of individual PTM on target proteins. To solve this problem, the genetic code expansion (GCE) strategy has been introduced into the field of PTM studies. Instead of modifying proteins after translation, GCE incorporates modified amino acids into proteins during translation, thus generating site-specifically modified proteins at target positions. In this review, we summarize the development of GCE systems for orthogonal translation for site-specific installation of PTMs.

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Profile of protein lysine propionylation in Aeromonas hydrophila and its role in enzymatic regulation

Cited by 2 publications

References 32 publications

A glimpse into novel acylations and their emerging role in regulating cancer metastasis

A glimpse into novel acylations and their emerging role in regulating cancer metastasis

Orthogonal Translation for Site-Specific Installation of Post-translational Modifications

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