Profile of secondary metabolites and genetic stability analysis in new lines of Echinacea purpurea (L.) Moench micropropagated via somatic embryogenesis

Lema-Rumińska, Justyna; Kulus, Dariusz; Tymoszuk, Alicja; Varejão, Jorge M.T.B.; Bahćevandžiev, Kiril

doi:10.1016/j.indcrop.2019.111851

Cited by 30 publications

(18 citation statements)

References 42 publications

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“…Also in the present study, RAPDs were more effective in detecting variation (3 out of 5 primers detected polymorphisms in 12 specimens) compared to ISSRs (only one primer). Those results coincide with the findings by Lema-Rumińska et al (2019) and Kulus et al (2019) in other members of the Asteraceae family. On the other hand, in the Poaceae family-Hordeum vulgare L. (Fernández et al 2002) and Oryza granulata Nees et Arn.…”

Section: Genetic and Phenotype Stability Of Agnps-treated Chrysanthemumssupporting

confidence: 92%

“…PCR was performed in a BioRad C1000 Touch thermal cycler with heated cover (Bio-Rad, Hercules, CA, USA) in the 25-µL reaction solution. The composition of reaction solution, PCR profiles, and electrophoretic separation of amplified DNA fragments were described in Lema-Rumińska et al (2019). Gel images were recorded using a GelDoc XR + Gel Photodocumentation System (Bio-Rad, Hercules, CA, USA) UV transilluminator with Image Lab 4.1 software.…”

Section: Genetic Stability Evaluationmentioning

confidence: 99%

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Silver nanoparticles induce genetic, biochemical, and phenotype variation in chrysanthemum

Tymoszuk

Kulus

2020

Plant Cell Tiss Organ Cult

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Despite the tremendous progress in breeding, novel and user-friendly techniques of plant improvement are desirable. The study aimed to analyze the usefulness of silver nanoparticles (AgNPs) in the breeding of chrysanthemum: one of the top ornamental plant species. In vitro regeneration of adventitious shoots from internodes of chrysanthemum ‘Lilac Wonder’ was induced on the modified Murashige and Skoog (MS) medium supplemented with 0.6 mg L−1 6-benzylaminopurine (BAP), 2 mg L−1 indole-3-acetic acid (IAA) and AgNPs at 0, 5, 10 and 20 ppm concentration. The efficiency of callogenesis and caulogenesis were analyzed after 10 weeks of culture. The concentration of chlorophylls, carotenoids, and phenolic compounds in shoots and calli were estimated. Plants obtained from 20 ppm AgNPs treatment were additionally analyzed on the genetic level using randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers. In vitro rooted shoots were acclimatized in the glasshouse and subjected to biochemical and phenotype stability evaluation. AgNPs at the highest concentration (20 ppm) suppressed both callogenesis and caulogenesis in vitro. The concentration of metabolites in callus was stable, regardless of AgNPs treatment, except for carotenoids which production was enhanced by 20 ppm AgNPs. In contrast, the content of chlorophyll a and b in shoots varied depending on AgNPs treatment. Polymorphic loci were detected in 12 and 9 AgNPs-treated-plants by RAPD and ISSR markers, respectively (one of which was common to both marker systems). Rooting and acclimatization were fully successful in all experimental combinations. Phenotype alternations were detected in six plants; one from 10 ppm AgNPs treatment and five from 20 ppm treatment. They included variation in pigment content (anthocyanins and carotenoids) and/or inflorescence shape. Interestingly, only two plants revealed both genetic and phenotype polymorphisms. No genetic or phenotype variation was detected in the control plants. In conclusion, AgNPs can be used in chrysanthemum breeding.

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Section: Genetic and Phenotype Stability Of Agnps-treated Chrysanthemumssupporting

confidence: 92%

Section: Genetic Stability Evaluationmentioning

confidence: 99%

Silver nanoparticles induce genetic, biochemical, and phenotype variation in chrysanthemum

Tymoszuk

Kulus

2020

Plant Cell Tiss Organ Cult

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“…Lema-Rumińska et al [6] used the MS medium containing BAP and α-Naphthalene Acetic Acid (NAA) for the somatic embryogenesis of E. purpurea that had been kept in darkness for 14 days. To stimulate the shoot proliferation of the embryos, the MS medium without auxin but containing cytokinin kinetin was used.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, the in vitro techniques can facilitate the genetic manipulation of secondary metabolites production [ 3 , 4 ]. The cultivation in an optimized medium under the controlled in vitro conditions is a viable alternative to the ex vitro cultivation of purple coneflower [ 5 , 6 ]. The regeneration of E. purpurea can occur through the embryogenesis followed by the organogenesis [ 3 , 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…The cultivation in an optimized medium under the controlled in vitro conditions is a viable alternative to the ex vitro cultivation of purple coneflower [ 5 , 6 ]. The regeneration of E. purpurea can occur through the embryogenesis followed by the organogenesis [ 3 , 5 , 6 ]. Somatic embryogenesis is one of the advanced techniques for the mass plant propagation from somatic (asexual) cells in vitro [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

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Indirect somatic embryogenesis of purple coneflower (Echinacea purpurea (L.) Moench): a medicinal-ornamental plant: evaluation of antioxidant enzymes activity and histological study

Dehestani-Ardakani¹,

Hejazi²,

Aliabad

2020

Mol Biol Rep

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Purple coneflower (Echinacea purpurea (L.) Moench) is a widely used medicinal and ornamental plant. In the present study, the callus embryogenesis was examined using benzyl adenine (BA) at three levels (3, 4, 5 mg L−1), 1-Naphthalene acetic acid (NAA) at three levels (0.1, 0.2 and 0.5 mg L−1) with or without activated charcoal (1 g L−1), coconut milk (50 ml L−1) and casein hydrolysate (50 mg L−1) in the MS (Murashige and Skoog 1962) medium. The embryogenesis indirectly occurred with the production of callus. The calli were observed in three forms: undifferentiated, embryogenic and organogenic. The embryogenic calli were dark green and coherent with a faster growth rate. The highest embryogenesis (100%) and embryonic regeneration (plantlet production) were obtained in the combined BA + NAA treatments with the activated charcoal, coconut milk and casein hydrolysate. However, the combined treatments of growth regulators failed to produce somatic embryos without the use of coconut milk and casein hydrolysate. The maximum amount of protein, peroxidase and catalase activity of embryogenic calli (2.02, 1.79 and 6.62ΔOD/Min/mg.protein, respectively), and highest percentage of acclimatization success (29.3% of plants) were obtained in the combined treatment of 5 mg L−1 BA + 0.5 mg L−1 NAA + activated charcoal + coconut milk + casein hydrolysate. The highest amount of chlorophyll content (33.3 SPAD value) and growth characteristics of acclimatized plantlets were observed in the media containing 3 mg L−1 BA + 0.1 and 0.2 mg L−1 NAA + 1 g. L−1 combined activated charcoal, coconut milk, casein hydrolysate. The histological studies confirmed the somatic embryogenesis in purple coneflower. Generally, it was found that the somatic embryogenesis of E. purpurea occurs at high levels of BA and low levels of NAA with the addition of coconut milk and casein hydrolysate.

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Overview of Somatic Embryogenesis

Ramírez-Mosqueda

2022

Methods in Molecular Biology

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Profile of secondary metabolites and genetic stability analysis in new lines of Echinacea purpurea (L.) Moench micropropagated via somatic embryogenesis

Cited by 30 publications

References 42 publications

Silver nanoparticles induce genetic, biochemical, and phenotype variation in chrysanthemum

Silver nanoparticles induce genetic, biochemical, and phenotype variation in chrysanthemum

Indirect somatic embryogenesis of purple coneflower (Echinacea purpurea (L.) Moench): a medicinal-ornamental plant: evaluation of antioxidant enzymes activity and histological study

Overview of Somatic Embryogenesis

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