Profiles and drivers of antibiotic resistance genes distribution in one-stage and two-stage sludge anaerobic digestion based on microwave-H2O2 pretreatment

Zhang, Junya; Liu, Jibao; Wang, Yawei; Yu, Dawei; Sui, Qianwen; Wang, Rui; Chen, Meixue; Tong, Juan; Wei, Yuansong

doi:10.1016/j.biortech.2017.05.157

Cited by 52 publications

(12 citation statements)

References 49 publications

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“…Previous work has also identified ermF as being among the most abundant ARGs in anaerobic digesters. 38,39 The predominant ARGs in the biomass sample (at 250 μg/L sulfamethoxazole) in order of normalized abundance were ermF, sul1, tetO, ermB, oxa-1, sul2, tetW, intI1, and ampC. Results indicated that oxa-1, ampC, tetO, and tetW were all significantly higher in biofilm samples (p < 0.031) compared to their normalized biomass ARG abundance.…”

Section: Methodsmentioning

confidence: 97%

Evaluating Antibiotic Resistance Gene Correlations with Antibiotic Exposure Conditions in Anaerobic Membrane Bioreactors

Zarei-Baygi

Harb

Wang

et al. 2019

Environ. Sci. Technol.

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Anaerobic membrane bioreactors (AnMBRs) are an emerging technology with potential to improve energy efficiency and effluent reuse in mainstream wastewater treatment. However, their contribution to the proliferation of contaminants of emerging concern, such as antibiotic resistance genes (ARGs), remains largely unknown. The purpose of this study was to determine the effect of select influent antibiotics at varying concentrations on the presence and abundance of ARGs in an AnMBR system and its effluent. Quantification of targeted ARGs revealed distinct profiles in biomass and effluent, with genes conferring resistance to different antibiotic classes dominating in biomass (macrolides) and effluent (sulfonamides). Effluent sul1 gene abundance was strongly correlated with abundance of intl1, signifying the potential importance of mobile genetic elements in ARG release from AnMBR systems. The addition of specific antibiotics also affected normalized abundances of their related ARGs, exemplifying the potential impact of selective pressures at both low (10 μg/L) and high (250 μg/L) influent antibiotic concentrations.

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Section: Methodsmentioning

confidence: 97%

Evaluating Antibiotic Resistance Gene Correlations with Antibiotic Exposure Conditions in Anaerobic Membrane Bioreactors

Zarei-Baygi

Harb

Wang

et al. 2019

Environ. Sci. Technol.

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“…Recent work has also demonstrated that HGT is promoted in subinhibitory concentrations of antibiotics [43] and that positive selection might even inhibit HGT by eliminating possible recipients in the environment [44]. However, the analysis of ARG transfer in WWTPs is seriously hindered by the lack of suitable methods for high-throughput assessment of ARG transfer under real WWTP conditions, so it is not surprising that there is a knowledge gap in our understanding of the transfer of ARGs in WWTPs [45,46].…”

Section: Transfer Of Antibiotic-resistance Genesmentioning

confidence: 99%

Antibiotic-Resistance Genes in Waste Water

Karkman

Thuy

Walsh

et al. 2018

Trends in Microbiology

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334

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Waste water and waste water treatment plants can act as reservoirs and environmental suppliers of antibiotic resistance. They have also been proposed to be hotspots for horizontal gene transfer, enabling the spread of antibiotic resistance genes between different bacterial species. Waste water contains antibiotics, disinfectants, and metals which can form a selection pressure for antibiotic resistance, even in low concentrations. Our knowledge of antibiotic resistance in waste water has increased tremendously in the past few years with advances in the molecular methods available. However, there are still some gaps in our knowledge on the subject, such as how active is horizontal gene transfer in waste water and what is the role of the waste water treatment plant in the environmental resistome? The purpose of this review is to briefly describe some of the main methods for studying antibiotic resistance in waste waters and the latest research and main knowledge gaps on the issue. In addition, some future research directions are proposed.

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“…A reduction of ARG/MGE relative abundance was obtained in most studies (p < 0.05 for 24 study conditions, p > 0.05 for 29 study conditions). It was not case for 8/28 studies, namely V060 [39], V116 [40], W013 [41], W037 [42], W041 [43], W054 [44], WN026 [45] and WN053 [46]. As for composting, Additional file 7: Table S5 summarized different study characteristics to explore heterogeneity in Fig.…”

Section: Effects On the Relative Abundance Of Arg And Mge (N = 71 Stumentioning

confidence: 99%

How effective are strategies to control the dissemination of antibiotic resistance in the environment? A systematic review

et al. 2020

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Background: Antibiotic resistance is a major concern for public and environmental health. The role played by the environment in disseminating resistance is increasingly considered, as well as its capacity for mitigation. We reviewed the literature on strategies to control dissemination of antibiotic-resistant bacteria (ARB), antibiotic resistance genes (ARG) and mobile genetic elements (MGE) in the environment. Methods: This systematic review focused on three main strategies: (i) restriction of antibiotic use (S1), (ii) treatments of liquid/solid matrices (S2) and (iii) management of natural environment (S3). Articles were collected from seven scientific databases until July 2017 and from Web of Science until June 2018. Only studies reporting measurements of ARB, ARG or MGE in environmental samples were included. An evidence map was drawn from metadata extracted from all studies eligible for S1, S2 and S3. Subsets of studies were assessed for internal and external validity to perform narrative and quantitative syntheses. A meta-analysis was carried out to assess the effects of organic waste treatments (random-effect models). Review findings: Nine hundred and thirty-one articles representing 1316 individual studies (n) were eligible for S1 (n = 59), S2 (n = 781) and S3 (n = 476) strategies, respectively. Effects of interventions to control the dissemination of antibiotic resistance in the environment were primarily studied in strategy S2. A partial efficiency of wastewater treatment plants (WWTPs) to reduce antibiotic resistance in treated effluent was reported in 118 high validity studies. In spite of the heterogeneity in published results, the meta-analysis showed that composting and drying were efficient treatments to reduce the relative abundance of ARG and MGE in organic waste, by 84% [65%; 93%] and 98% [80%; 100%], respectively. The effect of anaerobic digestion was not statistically significant (51% reduction [− 2%; 77%]) when organic waste treatments were compared together in the same model. Studies in strategies S1 and S3 mainly assessed the effects of exposure to sources of contamination. For instance, 28 medium/high validity studies showed an increase of antibiotic resistance in aquatic environments at the WWTP discharge point. Some of these studies also showed a decrease of resistance as the distance from the WWTP increases, related to a natural resilience capacity of aquatic environments. Concerning wildlife, nine medium/high validity studies showed that animals exposed to anthropogenic activities carried more ARB.

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Profiles and drivers of antibiotic resistance genes distribution in one-stage and two-stage sludge anaerobic digestion based on microwave-H2O2 pretreatment

Cited by 52 publications

References 49 publications

Evaluating Antibiotic Resistance Gene Correlations with Antibiotic Exposure Conditions in Anaerobic Membrane Bioreactors

Evaluating Antibiotic Resistance Gene Correlations with Antibiotic Exposure Conditions in Anaerobic Membrane Bioreactors

Antibiotic-Resistance Genes in Waste Water

How effective are strategies to control the dissemination of antibiotic resistance in the environment? A systematic review

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