Profiling Autoantibody Responses to Devise Novel Diagnostic and Prognostic Markers Using High-Density Protein Microarrays

Gupta, S.; Banerjee, Arghya; Syed, Parvez; Srivastava, Sanjeeva

doi:10.1007/978-1-0716-1562-1_14

Cited by 1 publication

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“…This perturbation subsequently results in defects in immune tolerance, inflammation, and cellular death mechanisms [11]. All these bioprocesses lead to the development of tumor-associated antigens (TA) that evoke an immune response and subsequent generation of antibodies against self-proteins, called TA autoantibodies (AAbs) [12][13][14]. The autoantibody production reflects greater immunological actions and advanced immunosurveillance in CRC patients.…”

Section: Introductionmentioning

confidence: 99%

A protein microarray‐based serum proteomic investigation reveals distinct autoantibody signature in colorectal cancer

Barpanda

Tuckley

Ray

et al. 2022

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Purpose Colorectal cancer (CRC) has been reported as the second leading cause of cancer death worldwide. The 5‐year annual survival is around 50%, mainly due to late diagnosis, striking necessity for early detection. This study aims to identify autoantibody in patients’ sera for early screening of cancer. Experimental Design The study used a high‐density human proteome array with approximately 17,000 recombinant proteins. Screening of sera from healthy individuals, CRC from Indian origin, and CRC from middle‐east Asia origin were performed. Bio‐statistical analysis was performed to identify significant autoantibodies altered. Pathway analysis was performed to explore the underlying mechanism of the disease. Results The comprehensive proteomic analysis revealed dysregulation of 15 panels of proteins including CORO7, KCNAB1, WRAP53, NDUFS6, KRT30, and COLGALT2. Further biological pathway analysis for the top dysregulated autoantigenic proteins revealed perturbation in important biological pathways such as ECM degradation and cytoskeletal remodeling etc. Conclusions and Clinical Relevance The generation of an autoimmune response against cancer‐linked pathways could be linked to the screening of the disease. The process of immune surveillance can be detected at an early stage of cancer. Moreover, AAbs can be easily extracted from blood serum through the least invasive test for disease screening.

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