Profiling Circulating Tumour Cells for Clinical Applications

Goh, Kah Yee; Lim, Darren Wan-Teck

doi:10.5772/intechopen.79228

Cited by 2 publications

(1 citation statement)

References 118 publications

(148 reference statements)

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“…In this context, enumeration and isolation of CTCs can be an alternative strategy for early cancer diagnosis in a less invasive manner. Moreover, this approach provides a real-time assessment of a patient condition as it simply demands a blood sample or other bodily uids 11 . In other words, the number of CTCs in the blood could be an indicator of the tumor response to a speci c therapy 12 .…”

Section: Introductionmentioning

confidence: 99%

Label-free isolation of circulating tumor cells using negative lateral dielectrophoresis-assisted inertial microfluidics

Mohammadi

Safari

Rahmanian

2023

Microfluid Nanofluid

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In this study, a dielectrophoresis (DEP)-assisted inertial micro uidics methodology was introduced for the the isolation of circulating tumor cells (CTCs) from diluted blood samples. The methodology was based on the negative DEP, provided with the laterally allocated microelectrodes as well as the proper selection of the applied electric eld frequency and voltage, to suppress the limited purity arising from the overlapped sizes of CTCs and white blood cells. Initially, the dynamics of 5 and 15 µm polystyrene microparticles within the DEP-assisted inertial micro uidic device were numerically and expimentally investigated. While the dynamics of the larger microparticles was governed by the inertial and DEP forces, those of the smaller microparticles were subject to the Dean drag force. In the absence of the DEP force, the larger microparticles migrate to two stable equilibrium positions corresponding to the upper and lower walls for the microchannel cross-section. In the presence of the DEP force, the equilibrium position corresponding to the lower wall is considerably displaced, while the equilibrium position corresponding to the top wall remains almost intact. Finally, it was found that the methodology outperformed the corresponding solely-inertial methodology in terms of purity for the isolation of CTCs from diluted blood samples. For instance, the purity of isolated MDA-MB-231 spiked in diluted blood samples, at a hematocrit of 1%, by the solely-inertial micro uidic device was 85.3%, while viable CTCs were captured using the DEP-assisted inertial micro uidic device with 94.1% purity at the total ow rate and applied voltage of, respectively, 650 µL min − 1 and 50 V.

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Section: Introductionmentioning

confidence: 99%

Label-free isolation of circulating tumor cells using negative lateral dielectrophoresis-assisted inertial microfluidics

Mohammadi

Safari

Rahmanian

2023

Microfluid Nanofluid

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Label-free isolation of circulating tumor cells using negative lateral dielectrophoresis- assisted inertial microfluidics

Mohammadi

Safari

Rahmanian

2023

Preprint

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In this study, a dielectrophoresis (DEP)-assisted inertial microfluidics methodology was introduced for the the isolation of circulating tumor cells (CTCs) from diluted blood samples. The methodology was based on the negative DEP, provided with the laterally allocated microelectrodes as well as the proper selection of the applied electric field frequency and voltage, to suppress the limited purity arising from the overlapped sizes of CTCs and white blood cells. Initially, the dynamics of 5 and 15 µm polystyrene microparticles within the DEP-assisted inertial microfluidic device were numerically and expimentally investigated. While the dynamics of the larger microparticles was governed by the inertial and DEP forces, those of the smaller microparticles were subject to the Dean drag force. In the absence of the DEP force, the larger microparticles migrate to two stable equilibrium positions corresponding to the upper and lower walls for the microchannel cross-section. In the presence of the DEP force, the equilibrium position corresponding to the lower wall is considerably displaced, while the equilibrium position corresponding to the top wall remains almost intact. Finally, it was found that the methodology outperformed the corresponding solely-inertial methodology in terms of purity for the isolation of CTCs from diluted blood samples. For instance, the purity of isolated MDA-MB-231 spiked in diluted blood samples, at a hematocrit of 1%, by the solely-inertial microfluidic device was 85.3%, while viable CTCs were captured using the DEP-assisted inertial microfluidic device with 94.1% purity at the total flow rate and applied voltage of, respectively, 650 µL min− 1 and 50 V.

show abstract

Profiling Circulating Tumour Cells for Clinical Applications

Cited by 2 publications

References 118 publications

Label-free isolation of circulating tumor cells using negative lateral dielectrophoresis-assisted inertial microfluidics

Label-free isolation of circulating tumor cells using negative lateral dielectrophoresis-assisted inertial microfluidics

Label-free isolation of circulating tumor cells using negative lateral dielectrophoresis- assisted inertial microfluidics

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