Profiling of bacteria by fast atom bombardment mass spectrometry

Heller, David; Cotter, Robert J.; Fenselau, Catherine; Uy, O. Manuel

doi:10.1021/ac00150a018

Cited by 154 publications

(105 citation statements)

References 13 publications

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“…Table 2 lists the fragmentation patterns observed for the selected peaks for each of the five bacteria (details of the MS/MS conditions are given above). The fragmentation patterns of the prominent peaks in the m/z 500 -800 region are suggestive of phospholipids since similar patterns have been observed using FAB on bacteria [8,9,39] and ESI-MS on bacterial phospholipids [24]. For example, the peak at m/z 705 in the E. coli UB5201 spectrum is separated by 141 mass units from another prominent peak at m/z 564, the latter appearing as a product ion in MS/MS when the precursor ion at m/z 705 is fragmented ( Table 2).…”

Section: Fi-esi Mass Spectra Of Crude Extracts and Biomarker Charactesupporting

confidence: 65%

“…However, the highest m/z value reproducibly attainable in the commonest pyrolysis MS instruments is very small (typically Ͻm/z 200), moreover, due to the in vacuo thermal degradation step essentially all information on the structure or identity of the molecules producing the pyrolysate is lost. Later, the application of desorption techniques, such as plasma desorption [7] and fast atom bombardment (FAB) [8,9], allowed the analysis of involatile fractions and extended the measurable mass range. The advent of "soft" ionization techniques, such as matrix-assisted laser desorption ionization (MALDI), and electrospray ionization (ESI) has enabled the mass spectrometric analysis of both large molecular weight compounds, such as proteins and nucleic acids [10,11], and low molecular weight metabolites [12], giving the potential for rapid characterization of microorganisms with greater accuracy, selectivity, and sensitivity for better discrimination.…”

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confidence: 99%

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Flow-injection electrospray ionization mass spectrometry of crude cell extracts for high-throughput bacterial identification

Vaidyanathan

Kell

Goodacre

2002

J. Am. Soc. Mass Spectrom.

101

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Flow-injection electrospray ionization mass spectrometry (FI-ESI-MS) of unfractionated cellfree extracts obtained from bacterial cells suspended in a solvent mixture was investigated as a rapid analytical method for reproducible, high-throughput bacterial identification. Five bacterial strains (two Escherichia coli, two Bacillus spp. and one Brevibacillus laterosporus) were studied in this investigation. Axenically grown bacterial cells were suspended in an acidic organic solvent and the cell-free extract was sequentially injected into a solvent flow stream that was sprayed into the ionization chamber of the ESI-MS. The spectra produced contained reproducible information, which was useful for discriminating between the bacteria. Tandem mass spectrometry was used to characterize further the peaks, and at least three classes of macromolecules, namely phospholipids, glycolipids, and proteins, were found to contribute most to the spectral information. Bacterial extracts stored under different conditions gave very similar mass spectra for each of the five bacterial strains, indicating that the extracts were stable even at room temperature for up to 24 h, with no loss of information content, which has obvious implications for automated high-throughput analysis. An analysis of the components of the extracting solvent mixture and their effects on the spectral information showed that acetonitrile contributes most significantly to the extraction process and hence to the information content of the spectra. (J Am Soc Mass Spectrom 2002, 13, 118 -128)

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Section: Fi-esi Mass Spectra Of Crude Extracts and Biomarker Charactesupporting

confidence: 65%

mentioning

confidence: 99%

Flow-injection electrospray ionization mass spectrometry of crude cell extracts for high-throughput bacterial identification

Vaidyanathan

Kell

Goodacre

2002

J. Am. Soc. Mass Spectrom.

101

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“…MALDI-TOF mass spectra of Salmonella lipids from the raw extract (A) and those doped with 2 mM of NaCl (B) and KCl (C). and the latter are the mono-sodium adduct species, referring to the previous study [35,36]. Series 1, which is also inhibited after the extract is doped with NaCl, is attributed to the protonated peaks for its 22 Da shift from Series 2.…”

Section: Resultssupporting

confidence: 53%

Rapid lipid profiling of bacteria by online MALDI-TOF mass spectrometry

Shu

Liang

et al. 2012

International Journal of Mass Spectrometry

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a b s t r a c tThe rapid identification of bacteria is of increasing interest in food, biosafety, and clinical analysis. In this paper, we report a technique for rapid lipid profiling of bacteria by online droplet-deposited MALDI mass spectrometry. In the experiment, lipids in bacterial cells (Escherichia coli, Salmonella, and Bacillus subtilis) are extracted with the mixture of chloroform/methanol via ultrasonication. The extracts are nebulized into suspended droplets and then instantly analyzed with an online droplet-deposited MALDI-TOF mass spectrometer. The lipid profiles of the three bacteria cultured in nutrient broth and LB (Lysogeny Broth) medium are obtained. The lipids phosphatidylethanolamine (PE), diglycosyl diacylglycerol (DGDG), and phosphatidylglycerol (PG) are observed in the MALDI-TOF mass spectra. The MALDI-TOF mass spectrum of each bacterial extract shows a characteristic profile. The experimental results demonstrate that the present technique is capable of conducting rapid lipid analysis and has the potential for rapid bioaerosol detection.

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“…They noticed that unique mass spectra were produced from bacterial extracts of different genera and of different species. In the 1980s, the development of desorption/ionization techniques such as plasma desorption (PD), laser desorption (LD) and fast atom bombardment (FAB) allowed the generation of molecular biomarker ions from microorganisms leading to bacterial profiling (Heller et al, 1987;Platt et al, 1988). In early experiments, only biomarker molecules of low molecular masses such as bacterial lipids were analysed (Shah & Collins, 1980;Heller et al, 1988), as the processes used for the ionization of biomolecules were too energy rich to avoid unpredictable analyte decomposition.…”

Section: Introductionmentioning

confidence: 99%

Applications of MALDI‐TOF Mass Spectrometry in Clinical Diagnostic Microbiology

Opota

Prod’hom

Greub

2017

MALDI‐TOF and Tandem MS for Clinical Microbiology

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Until recently, microbial identification in clinical diagnostic laboratories has mainly relied on conventional phenotypic and gene sequencing identification techniques. The development of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) devices has revolutionized the routine identification of microorganisms in clinical microbiology laboratories by introducing an easy, rapid, high throughput, low-cost, and efficient identification technique. This technology has been adapted to the constraint of clinical diagnostic laboratories and has the potential to replace and/or complement conventional identification techniques for both bacterial and fungal strains. Using standardized procedures, the resolution of MALDI-TOF MS allows accurate identification at the species level of most Gram-positive and Gram-negative bacterial strains with the exception of a few difficult strains that require more attention and further development of the method. Similarly, the routine identification by MALDI-TOF MS of yeast isolates is reliable and much quicker than conventional techniques. Recent studies have shown that MALDI-TOF MS has also the potential to accurately identify filamentous fungi and dermatophytes, providing that specific standardized procedures are established for these microorganisms. Moreover, MALDI-TOF MS has been used successfully for microbial typing and identification at the subspecies level, demonstrating that this technology is a potential efficient tool for epidemiological studies and for taxonomical classification.

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Profiling of bacteria by fast atom bombardment mass spectrometry

Cited by 154 publications

References 13 publications

Flow-injection electrospray ionization mass spectrometry of crude cell extracts for high-throughput bacterial identification

Flow-injection electrospray ionization mass spectrometry of crude cell extracts for high-throughput bacterial identification

Rapid lipid profiling of bacteria by online MALDI-TOF mass spectrometry

Applications of MALDI‐TOF Mass Spectrometry in Clinical Diagnostic Microbiology

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