Profiling of differential gene expression in the hypothalamus of broiler-type Taiwan country chickens in response to acute heat stress

Tu, Wei-Lin; Cheng, C. K.; Wang, Shih-Han; Tang, Pin‐Chi; Chen, Chih‐Feng; Chen, Hsin-Hsin; Lee, Yen-Pai; Chen, Shuen-Ei; Huang, San-Yuan

doi:10.1016/j.theriogenology.2015.09.028

Cited by 40 publications

(22 citation statements)

References 49 publications

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“…Acute heat stress‐treated hens were subjected to 38°C and 55% RH for 2 hr and were then allowed to recover at 25°C for 0 (H2R0), 2 (H2R2), and 6 hr (H2R6). Hens were killed after recovery and hypothalami were collected for protein analysis (Tu et al., ). After decapitation, the brain was carefully removed and then bisected perpendicular to the cortical surface at the caudal end of the hemisphere.…”

Section: Methodsmentioning

confidence: 99%

“…Previous studies explored global mRNA and protein expression in the testes and small yellow follicles of TCCs after acute heat stress (Cheng et al., , ; Wang et al., , , ). We have profiled differential gene expression in the hypothalamus of broiler‐type TCCs in response to acute heat stress (Tu et al., ). This study further analyzes the global protein profile to elucidate theregulatory response in the hypothalamus of TCCs after acute heat stress.…”

Section: Introductionmentioning

confidence: 99%

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Proteomic analysis of the hypothalamus of broiler‐type Taiwan country chickens in response to acute heat stress

Cheng

Chen

et al. 2018

Animal Science Journal

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The hypothalamus is a critical center for regulating heat retention or dissipation. This study investigated global protein changes in the hypothalamus of broiler-type Taiwan country chickens (TCCs) after acute heat stress. Twelve TCC hens aged 30 weeks were allocated to groups subjected to acute heat stress at 38°C for 2 hr without recovery, with 2 hr of recovery, and with 6 hr of recovery; a control group was maintained at 25°C. Hypothalami were collected for protein expression analysis at the end of each time point. The results showed 114 protein spots differentially expressed after acute heat stress. Most of the differentially expressed proteins were involved in cellular processes, metabolism, transport, and cellular component organization. Functional annotation analysis suggested that these proteins were related to cellular defensive responses against heat and oxidative stress, detoxification and toxin export/delivery, cytoskeleton integrity, oxygen transport, and neural development. The results of this study suggest that acute heat stress damages the hypothalamus of broiler-type TCCs through oxidative stress and provokes a series of responses to stabilize protein structures, degrade misfolded proteins, and remodel cytoskeletons for attenuating the detrimental effects by acute heat stress.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of the hypothalamus of broiler‐type Taiwan country chickens in response to acute heat stress

Cheng

Chen

et al. 2018

Animal Science Journal

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“…Negative controls were also run in each set of PCR assays, including without complementary DNA (cDNA) (no template controls, NTCs) and without reverse transcriptase. The fold expression or repression of the target gene relative to the internal control gene GAPDH (Tu et al 2015) in each sample was then calculated by the 2 -ΔΔCt method (Deo et al 2011). …”

Section: Methodsmentioning

confidence: 99%

Dynamic expression of HSP90B1 mRNA in the hypothalamus of two Chinese chicken breeds under heat stress and association analysis with a SNP in Huainan chickens

Wan¹,

Ma²,

Wei³

et al. 2017

Czech J. Anim. Sci.

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Wan Y., Ma C., Wei P., Fang Q., Guo X., Zhou B., Jiang R. (2017): Dynamic expression of HSP90B1 mRNA in the hypothalamus of two Chinese chicken breeds under heat stress and association analysis with a SNP in Huainan chickens. Czech J. Anim. Sci., 62, 82-87.The effects of heat stress on HSP90B1 messenger RNA (mRNA) expression in the hypothalamus of chicken were investigated and HSP90B1 variations were detected. Females of two Chinese chicken breeds (Huainan and Wenchang) were used for the experiments. At 64 days of age, the ambient temperature (24 ± 1°C) was increased to 35 ± 1°C for 24 h (heat stress), then decreased to 24 ± 1°C for 24 h (recovery). Hypothalamus samples were collected at 0, 12, and 24 h during heat stress, as well as 12 and 24 h during recovery. The HSP90B1 mRNA expression increased significantly during heat stress and significantly decreased during recovery being higher in Huainan chickens. Fifteen primer pairs were designed to amplify the exons of HSP90B1 by a polymerase chain reaction, and single nucleotide polymorphisms (SNPs) were detected by Sanger sequencing. In Huainan chickens, we identified a SNP (NC_006088.3:g.6798G>A) in exon 14 of HSP90B1 which did not cause amino acid variation but caused a codon for glutamic acid change from GAG to GAA. The frequencies for genotypes AA, GA, and GG were 0.49, 0.27, and 0.24, respectively. Individuals with the GG genotype survived heat stress at 42°C for a longer time (248.2 ± 39.3 min) than individuals with GA and AA genotypes, which survived for 227.2 ± 44.5 min and 179.3 ± 36.5 min, respectively. The results suggested that the increased heat tolerance was associated with the higher expression of HSP90B1, and genotype GG could be used as a potential marker for heat resistance in chickens.

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“…The hypothalamic transcriptome and proteome of the Huoyan goose [54, 263] and the hypothalamic transcriptome of Sichuan white goose [125] have been profiled before, during, or after their egg laying periods in the interests of finding clues to improve the reproductive performance of these economically valuable domestic animals (also see Figure 1 of [254). In the interests of optimizing feed intake in chickens or to understand how they cope with environmentally-induced pressures, many studies have also examined the role of body composition, fasting, diet, or heat stress on gene expression in chicken hypothalamus (e.g., [51, 106, 416, 439]; see also [232]). Despite the intensive investigations of chicken hypothalamus for molecular mining and extraction, these studies have not contextualized sub-regional changes in expression for molecules in relation to published stereotaxic atlases of the chicken that include illustrations, maps and drawings of the hypothalamus with stereotaxic coordinates [112, 444, 471].…”

Section: Molecular Mining Of the Hypothalamusmentioning

confidence: 99%

Mapping Molecular Datasets Back to the Brain Regions They are Extracted from: Remembering the Native Countries of Hypothalamic Expatriates and Refugees

Khan

Grant

Martínez

et al. 2018

Advances in Neurobiology

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Profiling of differential gene expression in the hypothalamus of broiler-type Taiwan country chickens in response to acute heat stress

Cited by 40 publications

References 49 publications

Proteomic analysis of the hypothalamus of broiler‐type Taiwan country chickens in response to acute heat stress

Proteomic analysis of the hypothalamus of broiler‐type Taiwan country chickens in response to acute heat stress

Dynamic expression of HSP90B1 mRNA in the hypothalamus of two Chinese chicken breeds under heat stress and association analysis with a SNP in Huainan chickens

Mapping Molecular Datasets Back to the Brain Regions They are Extracted from: Remembering the Native Countries of Hypothalamic Expatriates and Refugees

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