Profiling of Hydrophilic Proteins from Olea europaea Olive Pollen by MALDI TOF Mass Spectrometry

Abstract

The antigenic profile of Olea europaea pollen from different Mediterranean cultivars was obtained by MALDI mass spectrometry. A simple procedure of chemical fractionation of the whole antigen extract was developed, whereby less complex, or pure, fractions of antigen candidate were obtained prior to mass spectrometric analysis. Some of the features of protein structure and distribution probably depend on cultivar adaptation to the environment. The profilings of pollen proteins thus obtained allow the distinction of the analyzed cultivars into three distinct groups: (i) those characterized by a low Ole e 1 content; (ii) those over-enriched in Ole e 1 and (iii) that containing Ole e 3 and Ole e 7 only. The latter consists of at least four isoforms differing by the degree of glycosilation. These results demonstrate that the proposed experimental procedure, can supply valuable information on the antigens' micro heterogeneity.

Breaking the cells of rape bee pollen and consecutive extraction of functional oil with supercritical carbon dioxide

“…It is considered to be a natural health food which constitutes a potential source of energy and functional components for human consumption (Silva et al, 2006). A number of bioactive substances in bee pollen such as phenolics, flavonoids, anthocyanin, phospholipids and proteins have been reported (Nagata et al, 2005;Napoli et al, 2006;Di Paola-Naranjo, Sánchez-Sánchez, González-Paramás, & Rivas-Gonzalo, 2004;Tomas-Lorente, Garcia-Grau, Nieto, & Tomas-Barberan, 1992;Ukiya et al, 2003). Recently, increasing evidences prove its potential therapeutic benefits, such as free radical scavenging activities (Campos, Webby, Markham, Mitchall, & Da Cunha, 2003;Leja, Mareczek, Wyżgolik, Klepacz-Baniak, & Czekońska, 2007;Nagai, Inoue, Inoue, & Suzuki, 2002;Silva et al, 2006), inhibition of lipid peroxidation (Almaraz-Abarca, Campos, Ávila-Reyes, Naranjo-Jiménez, Corral, & González-Valdez, 2007) and suppressing the cellular and humoral response (Qin & Sun, 2005;Yuan & Xu, 1996).…”

Targeted proteomic approach in prostatic tissue: a panel of potential biomarkers for cancer detection

“…The frozen prostate tissue were powdered in liquid nitrogen. The powdered tissues were further homogenized in 1 mL of a cold solution containing 50mM NH 4 HCO 3 (pH 8), 0,05% SDS (v/v) and protease inhibitor cocktail (1:100, v/v), then submitted to sonication conditions 3 times for 10s/time [ 17 , 18 ]. Each operation was performed on ice.…”

Functional characterization of the partially purified Sac1p independent adenine nucleotide transport system (ANTS) from yeast endoplasmic reticulum

“…[Saccharomyces cerevisiae YJM1342] ( Supplementary Figs S2 and S3), sequence coverage of 19%. MS/MS (53,54) evaluation assigned a total of 2 peptides to the AJV32483.1, Imd2p (Saccharomyces cerevisiae YJM1342) ( Supplementary Fig. S4).…”