Profiling of naturally occurring folates in a diverse soybean germplasm by HPLC-MS/MS

Agyenim-Boateng, Kwadwo Gyapong; Zhang, Shengrui; Islam, Md. Shariful; Gu, Ying; Бин, Ли; Azam, Muhammad; Abdelghany, Ahmed M.; Qi, Jie; Ghosh, Suprio; Shaibu, Abdulwahab S.; Gebregziabher, Berhane S.; Feng, Yue; Li, Jing; Li, Yinghui; Zhang, Chunyi; Qiu, Lijuan; Liu, Zhangxiong; Liang, Qiuju; Sun, Junming

doi:10.1016/j.foodchem.2022.132520

Cited by 20 publications

(16 citation statements)

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“…Russian accessions followed by Chinese accessions contained significantly higher chlorophyll concentrations, while USA and Japanese accessions contained relatively lower concentrations ( Figure 1 ), attributed to the genetic variability of the accessions to perform in different environmental conditions, corresponding to the study of Song et al [ 35 ], who found that accessions from different geographical origins (such as Japanese and Chinese) are genetically distinct, resulting in various biochemical contents in soybean. In summary, understanding the origin of the germplasm and cultivation year is crucial in soybean breeding strategies to improve carotenoid contents, as suggested by several other studies on other desirable soybean seed nutritional compositions [ 18 , 20 , 21 , 22 ].…”

Section: Resultsmentioning

confidence: 99%

“…The chemical composition of soybean seeds can be affected by genotype, planting location, environmental conditions, cultivation year, maturity group (MG) [ 14 , 15 , 16 ] and seed coat color [ 17 ]. Some studies have investigated the influence of geographical origins in various nutritional components (including isoflavones, protein, oil, fatty acids, tocopherols and folates) of soybean varieties [ 12 , 13 , 18 , 19 , 20 , 21 ]. Similarly, other recent studies evaluated soybean accessions of different origins and maturity groups for their nutritional quality attributes such as isoflavone [ 21 ], fatty acid compositions [ 22 ] and tocopherols [ 23 ] and found variation among genotypes and between MGs.…”

Section: Introductionmentioning

confidence: 99%

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Origin, Maturity Group and Seed Coat Color Influence Carotenoid and Chlorophyll Concentrations in Soybean Seeds

Gebregziabher

Zhang

Ghosh

et al. 2022

Plants

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Soybean (Glycine max (L.) Merrill) seeds are abundant in physiologically active metabolites, including carotenoids and chlorophylls, and are used as an affordable source of functional foods that promote and maintain human health. The distribution and variation of soybean seed metabolites are influenced by plant genetic characteristics and environmental factors. Here, we investigated the effects of germplasm origin, genotype, seed coat color and maturity group (MG) on the concentration variation of carotenoid and chlorophyll components in 408 soybean germplasm accessions collected from China, Japan, the USA and Russia. The results showed that genotype, germplasm origin, seed color, and MG were significant variation sources of carotenoid and chlorophyll contents in soybean seeds. The total carotenoids showed about a 25-fold variation among the soybean germplasms, with an overall mean of 12.04 µg g−1. Russian soybeans yielded 1.3-fold higher total carotenoids compared with Chinese and Japanese soybeans. Similarly, the total chlorophylls were substantially increased in Russian soybeans compared to the others. Soybeans with black seed coat color contained abundant concentrations of carotenoids, with mainly lutein (19.98 µg g−1), β-carotene (0.64 µg g−1) and total carotenoids (21.04 µg g−1). Concentrations of lutein, total carotenoids and chlorophylls generally decreased in late MG soybeans. Overall, our results demonstrate that soybean is an excellent dietary source of carotenoids, which strongly depend on genetic factors, germplasm origin, MG and seed coat color. Thus, this study suggests that soybean breeders should consider these factors along with environmental factors in developing carotenoid-rich cultivars and related functional food resources.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Origin, Maturity Group and Seed Coat Color Influence Carotenoid and Chlorophyll Concentrations in Soybean Seeds

Gebregziabher

Zhang

Ghosh

et al. 2022

Plants

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“…Folate monoglutamates, including tetrahydrofolate (THF), 5-methyltetrahydrofolate (5MTHF), 5,10-methenyltetrahydrofolate (5,10MTHF), 10-formylfolic acid (10FFA), 5-formyltetrahydrofolate (5FTHF), dihydrofolate (DHF) and folic acid (FA), were extracted and quantified using an HPLC-MS/MS system described previously ( 13 ). Carotenoids, including beta-carotene (β-carotene), alpha-carotene (α-carotene), lutein, zeaxanthin, and beta-cryptoxanthin (β-cryptoxanthin) were analyzed as described by Gebregziabher et al ( 14 ).…”

Section: Methodsmentioning

confidence: 99%

The nutritional composition of the vegetable soybean (maodou) and its potential in combatting malnutrition

Agyenim-Boateng

Zhang

et al. 2023

Front. Nutr.

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IntroductionGlobal malnutrition continues to be a canker owing to poor eating habits and over-reliance on the major staple crops. Vegetable soybean (maodou) is gaining popularity globally as an affordable snack and vegetable.MethodsIn this study, we profiled the nutritional composition of 12 soybean cultivars at the vegetable (R6-R7) and mature (R8) stages. We also conducted an RNA-seq analysis during seed development, focusing on key biosynthesis enzymes for quality traits.ResultsThe results showed that 100 g of maodou contained 66.54% moisture, 13.49% protein, 7.81% fatty acids, 2.47% soluble sugar, abundant content of minerals, and micronutrients, including folate (462.27 μg FW) and carotenoids (3,935.41 μg FW). Also, the isoflavone content of maodou ranged between 129.26 and 2,359.35 μg/g FW. With regard to the recommended daily allowance, 100 g fresh weight of maodou can contribute 26.98, 115.57, and 11.60% of protein, folate, and zinc, respectively, and significant proportions of other nutrients including linoleic acid (21.16%), linolenic acid (42.96%), zinc (11.60%), and iron (18.01%). On a dry weight basis, maodou has two to six folds higher contents of folate, tocopherol, and carotenoid than the mature soybean. Furthermore, RNA-seq analysis revealed that key biosynthesis enzymes of quality traits are differentially expressed during seed development and may contribute to variations in the content of quality traits at the vegetable and mature stages. Correlation analysis of quality traits at both stages revealed that protein only correlated positively with zinc at the vegetable stage but negatively correlated with total tocopherol and total fatty acid at the mature stage. Complex associations among folates, soluble sugar, and isoflavones were also identified.DiscussionThis study provides insight into the nutritional contents of vegetable soybean and demonstrates that maodou is essential for meeting the nutritional requirements of most countries.

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“…Genotypes and environment significantly influence the folate content in cereal. For instance, total folate content among wheat and soybean genotypes shows a remarked variation ( 7 , 8 ). Moreover, most of the folate in cereal products also differs markedly according to the growing conditions (climate, soil type, weather conditions, etc.)…”

Section: Introductionmentioning

confidence: 99%

Foxtail millet [Setaria italica (L.) P. Beauv.] grown under nitrogen deficiency exhibits a lower folate contents

et al. 2023

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Foxtail millet [Setaria italica (L.) P. Beauv.], as a rich source of folates, has been cultivated on arid infertile lands, for which N deficiency is one of the major issues. Growing environments might have a significant influence on cereal folate levels. However, little is known whether N deficiency modulates cereal folate levels. In order to obtain enriched folate foxtail millet production in nutrient-poor soil, we conducted a study investigating the content of folate derivatives of 29 diverse foxtail millet cultivars under two N regimes (0 and 150 kg N ha−1) for 2 years to explore folate potential grown under low N. The contents of total folate and most derivatives were reduced by N deficiency. The effect on total folate content caused by N was stronger than cultivar genotype did. Folate content of enriched folate cultivars was prone to be reduced by N deficiency. Structural equation models (SEMs) revealed that N fertilization had a positive indirect effect on grain folate content through influencing plant N and K accumulation. Collectively, the results indicate much more attention should be paid to N management when foxtail millet is cultivated in infertile soil, to improve foxtail millet folate contents.

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Profiling of naturally occurring folates in a diverse soybean germplasm by HPLC-MS/MS

Cited by 20 publications

References 39 publications

Origin, Maturity Group and Seed Coat Color Influence Carotenoid and Chlorophyll Concentrations in Soybean Seeds

Origin, Maturity Group and Seed Coat Color Influence Carotenoid and Chlorophyll Concentrations in Soybean Seeds

The nutritional composition of the vegetable soybean (maodou) and its potential in combatting malnutrition

Foxtail millet [Setaria italica (L.) P. Beauv.] grown under nitrogen deficiency exhibits a lower folate contents

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