Profiling of plasma metabolites in postmenopausal women with metabolic syndrome

Abstract

Objective:The aim of the study was to investigate the associations of amino acids and other polar metabolites with metabolic syndrome (MetS) in postmenopausal women in a lean Asian population.Methods:The participants were 1,422 female residents enrolled in a cohort study from April to August 2012. MetS was defined according to the National Cholesterol Education Program Adult Treatment Panel III modified for Japanese women. Associations were examined between MetS and 78 metabolites assayed in fasting plasma samples using capillary electrophoresis-mass spectrometry. Replication analysis was performed to confirm the robustness of the results in a separate population created by random allocation.Results:Analysis was performed for 877 naturally postmenopausal women, including 594 in the original population and 283 in the replication population. The average age, body mass index, and levels of high- and low-density lipoprotein cholesterol of the entire population were 64.6 years, 23.0 kg/m2, 72.1 mg/dL, and 126.1 mg/dL, respectively. There was no significant difference in low-density lipoprotein cholesterol levels between women with and without MetS. Thirteen metabolites were significantly related to MetS: multiple plasma amino acids were elevated in women with MetS, including branched-chain amino acids, alanine, glutamate, and proline; and alpha-aminoadipate, which is generated by lysine degradation, was also significantly increased.Conclusions:Our large-scale metabolomic profiling indicates that Japanese postmenopausal women with MetS have abnormal polar metabolites, suggesting altered catabolic pathways. These results may help to understand metabolic disturbance, including in persons with normal body mass index and relatively high levels of high-density lipoprotein cholesterol, and may have clinical utility based on further studies.

Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applications

“…Biomarker identification is performed in different biological fluids, blood being one of the most promising amongst them. In recent studies, metabolites and especially amino acids were identified as biomarkers in blood for amyotrophic lateral sclerosis , chronic kidney disease (CKD) , major depressive disorder , onchocercias infection , osteoporosis , Parkinson's disease , pancreatic cancer , urinary tract symptoms and metabolic syndrome in postmenopausal women by CZE‐MS. For identification and accurate quantification of biomarkers for inborn errors of metabolism from dried blood spots, DiBattista et al.…”

Association between dyslipidemia and plasma levels of branched-chain amino acids in the Japanese population without diabetes mellitus

“…15 Dietary intake was assessed using a food frequency questionnaire. 16 Height and weight were measured with the participant wearing light clothing. Body mass index was calculated as weight in kilograms divided by the squared height in meters.…”

“…Hypertension was defined as the use of any antihypertensive drug, systolic bold pressure $140 mmHg, or diastolic blood pressure $90 mmHg. 15,16 Blood samples were collected early in the morning (after the participant had fasted overnight) and then centrifuged to obtain plasma for measuring BCAA levels and serum for measuring lipid levels and other indicators. Plasma BCAA levels were quantified using capillary electrophoresis timeof-flight mass spectrometry (CE-MS) according to a previously reported protocol.…”

Recent advances in amino acid analysis by capillary electromigration methods: June 2015–May 2017

“…The plasma from 1422 Asian women suffering from metabolic syndrome was analysed. A special focus was placed on AA and polar metabolites . Thirty metabolites were related to metabolic syndrome.…”