Profiling of the Candidate Interacting Proteins of SELF-PRUNING 6A (SP6A) in Solanum tuberosum

Abstract

SELF-PRUNING 6A (SP6A), a homolog of FLOWERING LOCUS T (FT), has been identified as tuberigen in potato. StSP6A is a mobile signal synthesized in leaves and transmitted to the stolon through phloem, and plays multiple roles in the growth and development of potato. However, the global StSP6A protein interaction network in potato remains poorly understood. In this study, BK-StSP6A was firstly used as the bait to investigate the StSP6A interaction network by screening the yeast two-hybrid (Y2H) library of potato, resulting in the selection of 200 independent positive clones and identification of 77 interacting proteins. Then, the interaction between StSP6A and its interactors was further confirmed by the Y2H and BiFC assays, and three interactors were selected for further expression analysis. Finally, the expression pattern of Flowering Promoting Factor 1.1 (StFPF1.1), No Flowering in Short Days 1 and 2 (StNFL1 and StNFL2) was studied. The three genes were highly expressed in flowers or flower buds. StFPF1.1 exhibited an expression pattern similar to that of StSP6A at the stolon swelling stages. StPHYF-silenced plants showed up-regulated expression of StFPF1.1 and StSP6A, while expression of StNFL1 and StNFL2 was down-regulated in the stolon. The identification of these interacting proteins lays a solid foundation for further functional studies of StSP6A.

StBIN2 Positively Regulates Potato Formation through Hormone and Sugar Signaling

“…In other words, BIN2's regulation of hormone signals in the process of potato formation is not unitary but ultimately promotes the expression of key genes in potato tuber formation to promote potato formation. In addition, it has been reported that the interaction between SP6A and SWEET11 can promote its enzyme activity [35]. In this experiment, protein interaction in potatoes was confirmed, and the activity of SWEET protein in StBIN2-overexpressing potatoes was also significantly increased in the predominant tuber-expansion stage, which also promoted potato formation.…”

“…ABI5 and SP6A (a FT family member) interact with each other to regulate potato tuber development [34]. In addition, the sugar transporter SWEET11 potentially interacts with SP6A [35].…”

PEBP Signaling Network in Tubers and Tuberous Root Crops

“…In Arabidopsis , BRANCHED1 interacts with FT but not TFL1 to prevent floral transition in the axillary meristem [ 129 ], suggesting a conserved regulatory network across plant species. StSP6A was also found to interact with Flowering-Promoting Factor 1, No Flowering in Short Days 1 (StNFL1), and StNFL2, which are likely crucial for tuber formation [ 130 ].…”

Screening and verification of proteins that interact with the anthocyanin-related transcription factor PbrMYB114 in ‘Yuluxiang’ pear

“…The yeast two-hybrid (Y2H) system is a valuable tool for swiftly detecting novel protein-protein interactions across various organisms ( Rodriguez-Negrete, Bejarano & Castillo, 2014 ). This approach has been effectively utilized to discover potential interactors for numerous key plant proteins ( Kong et al, 2019 ; Wang et al, 2022 , 2021b , 2021c ). For instance, a Y2H screen using an apple cDNA library identified MdBT2, a nitrate-responsive protein interacting with MdMYB1 and influencing anthocyanin accumulation ( Wang et al, 2018b ).…”