Profilometric and Morphometric Response of Murine Skin to Cosmeceutical Agents

Abstract

The pronounced effects noted with all 3 compounds indicate that topical agents other than retinoic acid may have comparative stimulating effects on the skin in nonirradiated mice.

Effect of topical clay application on the synthesis of collagen in skin: an experimental study

“…For example, a similar protocol applying retinoid at a higher dosage (0.1%) in human skin led to a slow and gradual increase in collagen 14 . Contrarily, RA has been described as a potent and rapid stimulator of collagen in photoaged human skin, 3 however in mice, application of RA 0.05% for 2 weeks was sufficient to reduce wrinkles 7 …”

“…Retinoids can also influence DNA repair and gene expression to increase ECM production 6 . Application of RA 0.05% for 2 weeks promoted wrinkle reduction in rats 7 …”

“…6 Application of RA 0.05% for 2 weeks promoted wrinkle reduction in rats. 7 Natural products are becoming more popular in cosmetic clinics, 8 with clay being a popular option. 9 However, there is little information about the functional effects of clay upon the skin layers to support its use.…”

A close relationship between type 1 diabetes and vitamin A-deficiency and matrix metalloproteinase and hyaluronidase activities in skin tissues

“…On the other hand, skin epidermis in vitamin A‐deficient rats is thinner as compared with control rats (35,36). In contrast, RA‐treated skins show an increase in epidermal thickness by hyperplasias of stratum spinosum as compared with control skins (25,27,32,37). In the current study, we showed that type 1 diabetic rats are vitamin A‐deficient.…”

Cutaneous injury following acute UV-B radiation in a mouse model: a pilot histological study

“…Morphometry was performed to obtain the following data: (1) epidermal width; (2) total dermal width; (3) ratio of collagen layer to total dermis; (4) ratio of collagen I: collagen III from Herovici polychrome stain preparation (red and blue fibers); (5) count of mast cells; (6) intersection pointcounting to determine fractional area of elastic fibers; 14 and (7) count of PCNA-positive cells to determine epidermal proliferation index. 15,16 Quantitative results are expressed as means¡ standard deviation, and differences between mean scores were analyzed by non-parametric KruskalWallis k-sample test using SPSS16.0 and Excel softwares.…”