Prognosis in human glioblastoma based on expression of ligand growth hormone-releasing hormone, pituitary-type growth hormone-releasing hormone receptor, its splicing variant receptors, EGF receptor and PTEN genes

Abstract

Results expression of ghRh was found to be positive in 61.9 % of samples. pghRh receptor was not expressed in our sample set, while sV1 could be detected in 17.4 % and sV2 in 8.6 % of the gB tissues. In 65.2 and 78.3 % of samples, significant egFR over-expression or Pten under-representation could be detected, respectively. In 47.8 % of cases, egFR up-regulation and Pten down-regulation occurred together. survival was significantly poorer in tumors lacking ghRh expression. this worse prognosis in ghRh negative group remained significant even if sV1 was also expressed. Conclusion Our study shows that ghRh and sV1 genes expressed in human gB samples and their expression patterns are associated with poorer prognosis.Keywords ghRh · pghRhR · splice variants · sV1 · egFR · Pten · human glioblastoma AbstractPurpose glioblastoma (gB) is the most frequent brain tumor. Despite recent improvement in therapeutic strategies, the prognosis of gB remains poor. growth hormone-releasing hormone (ghRh) may act as a growth factor; antagonists of ghRh have been successfully applied for experimental treatment of different types of tumors. the expression profile of ghRh receptor, its main splice variant sV1 and ghRh have not been investigated in human gB tissue samples. Methods We examined the expression of ghRh, fulllength pituitary-type ghRh receptor (pghRhR), its functional splice variant sV1 and non-functional sV2 by Rt-PCR in 23 human gB specimens. epidermal growth factor receptor (egFR) and phosphatase and tensin homolog gene (Pten) expression levels were also evaluated by quantitative Rt-PCR. Correlations between clinico-pathological parameters and gene expressions were analyzed.g. Mezey and a. treszl contributed equally to this work.

Effect of marital status on survival in glioblastoma multiforme by demographics, education, economic factors, and insurance status

“…Fourth, married patients might be diagnosed at an earlier stage, which would also partially contribute to better survival. Fifth, marriage influences the function of body physiologically, partially through modulating the level of endocrinal hormones that affect the prognosis of GBM 19, 20, 21. In addition, the offspring of married couples provide additional support that is often unavailable to single patients 22.…”

Glioblastoma cell migration is directed by electrical signals

“…This produces asymmetrical membrane protrusions and consequently migration in the direction of the cathode. This pathway is frequently dysregulated in GBM through EGFR overexpression (65% of GBM), and downregulation of the Akt inhibitor PTEN (78% of GBM) [30].…”

RNA processing as an alternative route to attack glioblastoma

“…GHRH receptor has two splice isoforms, the functional SV1 and the non-functional SV2. GBM patients, whose tumors lack GHRH expression, present poor prognosis, while GHRH positive and SV1 negative patients showed a better prognosis (Mezey et al 2014).…”