Prognostic Impact of miR-200 Family Members in Plasma and Exosomes from Tumor-Draining versus Peripheral Veins of Colon Cancer Patients

Abstract

Objective: To evaluate the prognostic potential of expression levels of miR-200 family members (miR-200a, miR-200b, miR-200c, miR-429, miR-141) in plasma and exosomes from the tumor-draining vein (mesenteric vein [MV]) and peripheral vein (PV) of colon cancer (CC) patients. Methods: We analyzed the expression of miR-200 family members in matched samples of MV and PV plasma from 50 resected patients with CC and correlated our findings with overall survival (OS). We also examined the content of these microRNAs in MV and PV exosomes. Results: Expression levels were higher in MV than in PV (miR-200a, p < 0.001; miR-200b, p < 0.001; miR-429, p = 0.01; miR-200c, p = 0.05; miR-141, p = 0.05). Low levels of both miR-200c and miR-141 in MV plasma were associated with longer OS (p = 0.02). This association was maintained for the MV exosome cargo of miR-200c and miR-141 (p = 0.02). Conclusion: Our findings provide the first indication that expression levels of miR-200c and miR-141 in MV plasma can identify CC patients with poor prognosis. In addition, our results lend further support to the premise that tumor-draining veins constitute a better source of biomarkers than do PVs.

Exosomal MALAT1 sponges miR-26a/26b to promote the invasion and metastasis of colorectal cancer via FUT4 enhanced fucosylation and PI3K/Akt pathway

“…Exosomal LncRNAs are transcribed to regulate the expression of oncogenes and tumor suppressor genes in tissue-and cell-specific manners [17]. Ren et al quantitatively detected that carcinoma-associated fibroblasts promoted the stemness and chemoresistance of CRC by transferring exosomal lncRNA H19 [15]. Here, MALAT1 was proved to reside in the lumen area of CRC exosomes and exosomal MALAT1 derived from metastatic CRC cells could be transferred and regulate the expression of FUT4 in recipient cells, as a competing endogenous RNA for miR-26a and miR-26b, which regulated malignant traits of primary CRC cells.…”

“…Exosomes also potentially participate in the development and progression of CRC. A recent study provides a novel notion that miR-200c and miR-141 contain in exosomes of mesenteric vein plasma could predict colon cancer patients with poor prognosis [15]. Exosomes derived from bone marrow-derived mesenchymal stem/stromal cells also enlarge the population of colon cancer stem cells by treating colon cancer cells (HCT-116, HT-29 and SW480) [16].…”

Monitoring Therapy Efficiency in Cancer through Extracellular Vesicles

“…Taken together, these results suggest a link between decrease and therapy success, implicating that EpCAM+ MPs can be used to monitor therapy response. Santasusagna et al [41] (Table 1) evaluated the potential of vesicular miRNA to predict therapeutic success in 50 CRC resected patients. Expression levels of miR-200 family was analyzed in EVs (from peripheric and mesenteric blood) and correlated with overall survival (OS).…”

“…Expression levels of miR-200 family was analyzed in EVs (from peripheric and mesenteric blood) and correlated with overall survival (OS). The group saw an association between low levels of miR-200c and miR-14 and significant longer OS, thus identifying two vesicular miRNAs as predictor for therapy failure and poor prognosis [41]. If primarily not resectable in a curative manner, neoadjuvant chemo-radiation therapy (CRT) can be followed by response assessment to evaluate surgical options.…”

Integrated characterization and validation of the prognostic significance of microRNA-200s in colorectal cancer

“…A total of 381 records were identified through systematic search and manual review for initial search. On the basis of the study selection and exclusion criteria, finally, a total of 11 articles including 30 studies were found to be eligible and enrolled in the data synthesis [23][24][25][26][27][28][29][30][31][32][33]. Overall, all enrolled studies obtained a NOS score more than 6, which revealed a moderately satisfactory quality.…”