Prognostic Model of Pulmonary Adenocarcinoma by Expression Profiling of Eight Genes As Determined by Quantitative Real-Time Reverse Transcriptase Polymerase Chain Reaction

Endoh, Hideki; Tomida, Shuta; Yatabe, Yasushi; Konishi, Hiroyuki; Osada, Hirofumi; Tajima, Kohei; Kuwano, Hiroyuki; Takahashi, Takashi; Mitsudomi, Tetsuya

doi:10.1200/jco.2004.04.109

Cited by 151 publications

(126 citation statements)

References 32 publications

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“…30 Despite this, examples of reliable validation of microarray results by PCR also exist. 31,32 In the present study, we applied real-time Q-PCR to validate cDNA microarray results of Dyrskjøt et al 16 The highly discriminative gene expression pattern of the 26 genes presented by Dyrskjøt et al could not be reproduced. This poor reproducibility may have several reasons.…”

Section: Discussionmentioning

confidence: 99%

Prediction of recurrence in Ta urothelial cell carcinoma by real‐time quantitative PCR analysis: A microarray validation study

Schultz

Wester

Straatman

et al. 2006

Intl Journal of Cancer

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Accurate prediction of tumor recurrence in patients with superficial urothelial cell carcinoma (UCC) might result in a significant reduction of invasive follow-up cystoscopies. A recent study identified a panel of 26 genes from a large cDNA microarray analysis of bladder tumors that discriminated between early-and late-recurring patients with superficial Ta tumors (Dyrskjøt et al., Nat Genet 2003;33:90-6). We aimed to validate this panel of genes in 44 primary Ta UCCs (23 and 21 tumors from patients with short or prolonged recurrence-free periods, respectively), by real-time quantitative PCR. Statistical analysis showed marginal significant different mRNA expression levels between the 2 patient groups. To evaluate a supplementary effect of genes for the identification of patients with short or prolonged recurrence-free intervals, forward logistic regression analysis was applied. This revealed that a combination of the expression profiles of the genes HNRPK, LTB4DH and ANP32B resulted in the best performance, although the combination only marginally increased the predictive value of HNRPK alone. Comparing the receiver-operating-characteristic curves for HNRPK expression among patients with short or prolonged recurrence-free periods, revealed an area under the curve of 0.696 (95% CI, 0.537-0.855). Using the median HNRPK expression level as cut-off, a sensitivity of 69.6% and a specificity of 71.4% were obtained for the identification of patients with short or prolonged recurrence-free periods, respectively. In conclusion, we were not able to confirm the microarray gene expression pattern of the 26 genes shown by Dyrskjøt et al. The discovery of accurate recurrence predictive markers, therefore, remains a challenge. ' 2006 Wiley-Liss, Inc.Key words: recurrence; urothelial cell carcinoma; real-time quantitative PCR; microarray; validation Urothelial cell carcinoma (UCC) comprises up to 95% of human bladder cancer. It originates in the urothelium, the layer of cells lining the inner bladder wall. The larger part of patients with UCC is diagnosed with superficial tumors, pTNM stages Ta and T1, of which the depth of organ invasion is limited to the stromal mucosa. These tumors can be removed relatively easy by transurethral resection. Unfortunately, 70% of these patients experience recurrences after tumor removal and some will show progression towards muscle invasive disease. This necessitates frequent examination of the bladder by cystoscopy, which is invasive and represents a burden to the patient. The monitoring of these patients also includes those who will remain recurrence-free for several years or even for the rest of their lives. This generates a substantial number of unnecessary cystoscopies, which may be prevented by accurate prediction of tumor recurrence (or nonrecurrence) in patients with superficial UCC.The current standards for the estimation of the risk of recurrence are tumor grade, size and multiplicity. 1-3 Although these factors are relatively accurate in the group of patients with superficial UCC,...

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Section: Discussionmentioning

confidence: 99%

Prediction of recurrence in Ta urothelial cell carcinoma by real‐time quantitative PCR analysis: A microarray validation study

Schultz

Wester

Straatman

et al. 2006

Intl Journal of Cancer

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“…Although previous studies have reported a decrease in PTK7 expression levels in metastatic melanoma (25) and clear cell renal cell carcinoma, (26) PTK7 has been shown to be upregulated in many types of human malignancy, including colon cancer, lung carcinoma, liposarcoma, gastric cancer, and acute myeloid leukemia. (11,(17)(18)(19)(20)22) Prebet et al (20) reported that increased expression of PTK7 is correlated with poor clinical outcome in acute myeloid leukemia, a finding that was linked to increased cell migration and resistance to apoptosis. Meng et al (23) showed that knockdown of PTK7 in colon cancer cells inhibits proliferation and induces caspase-10-dependent apoptosis through the mitochondrial pathway.…”

Section: Discussionmentioning

confidence: 99%

“…Increased PTK7 expression has been reported in several types of cancer, including colon cancer, (11,17) gastric cancer, (18) lung cancer, (19) acute myeloid leukemia, (20,21) and liposarcoma. (22) Expression of PTK7 in leukemia cells enhances cell migration and survival.…”

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confidence: 99%

Oncogenic role of protein tyrosine kinase 7 in esophageal squamous cell carcinoma

et al. 2013

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Esophageal squamous cell carcinoma (ESCC) is a common subtype of esophageal cancer that is particularly prevalent in East Asian countries. Our previous expression profile analysis showed that the gene encoding protein tyrosine kinase 7 (PTK7) is upregulated in ESCC tissues. Here, we aimed to validate PTK7 as a prognostic factor and a candidate target for molecular treatment of ESCC. Both RT-PCR and Western blot analysis of tissues from ESCC patients revealed that PTK7 was significantly upregulated in tumor tissue samples of ESCC. Immunohistochemical staining of PTK7 showed that increased expression of PTK7 was inversely correlated with overall survival (P = 0.021). In vitro knockdown of PTK7 inhibited proliferation, survival, wound healing, and invasion of ESCC cells. In addition, PTK7 knockdown decreased phosphorylation of Akt, Erk, and focal adhesion kinase (FAK), important determinants of cell proliferation, survival, and migration. Therefore, our findings suggest that PTK7 has potential as a prognostic marker for ESCC and might also be a candidate for targeted therapy in the treatment of ESCC. (Cancer Sci 2013; 104: 1120-1126 E sophageal cancer is one of the most devastating malignancies. Despite recent developments in its management, advanced esophageal cancer is associated with high rates of local recurrence and distant metastasis.(1,2) Although there has been improvement in clinical outcome through multidisciplinary treatment strategies, (3)(4)(5) such as total mediastinal lymph node dissection, (6) and new diagnostic modalities such as positron emission tomography, (7) these developments have not proven satisfactory in preventing the high recurrence rates. Currently, the prognosis is only promising if the tumor is detected early and resected completely.Esophageal squamous cell carcinoma (ESCC) is the most common histologic subtype of esophageal cancer in East Asian countries.(8) It has a unique profile of clinical and anatomical characteristics that is distinct from those of esophageal adenocarcinoma, which is more prevalent in North America, UK, and Australia.(8) To develop prognostic diagnosis and effective therapeutics for ESCC, the identification of biomarkers is of vital importance. In a gene expression profiling analysis to pursue biomarkers, we identified protein tyrosine kinase 7 (PTK7; also known as colon carcinoma kinase-4 or CCK-4), as a candidate biomarker for ESCC. (9) Protein tyrosine kinase 7 is a receptor tyrosine kinase-like molecule containing an extracellular domain with seven immunoglobulin-like loops, a transmembrane domain, and a defective tyrosine kinase domain that resembles a catalytic domain but lacks catalytic activity.(10-12) Mice expressing a truncated form of PTK7 protein die perinatally, revealing a defect in neural tube closure and stereociliary bundle orientation. These findings implicate PTK7 as a regulator of planar cell polarity (PCP).(13) It has been shown that PTK7 recruits RACK1, which affects Dsh recruitment by interaction with PKCd1. (14) Interaction between PTK7...

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“…The TaqMan probes (Hs01070032_ m1 Jag1, Hs00171432_m1 Jag2, Hs00194509_m1 Dll1, Hs01085096_m1 Dll3, Hs00184092_m1 Dll4, Hs00172878_m1 Hes1, Hs01062014_m1 Notch1) and the TaqMan Assay kit (all from Applied Biosystems; Thermo Fisher Scientific, Inc.) were used to perform PCR. The expression of the above-mentioned genes [by the change-in-cycling-threshold ΔCq method (18,19)] were calculated and normalized to ribosomal 18SRNA gene expression (Hs99999901_s1 18SRNA). The following thermocycling conditions were used: 50˚C for 2 min; 95˚C for 10 min; 40 cycles of 95˚C for 15 sec and 60˚C for 60 sec.…”

Section: Quantitative Polymerase Chain Reaction (Qpcr)mentioning

confidence: 99%

Prognostic significance of Notch ligands in patients with non-small cell lung cancer

Pancewicz-Wojtkiewicz

Eljaszewicz

Kowalczuk

et al. 2016

Oncology Letters

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Prognostic Model of Pulmonary Adenocarcinoma by Expression Profiling of Eight Genes As Determined by Quantitative Real-Time Reverse Transcriptase Polymerase Chain Reaction

Abstract: By analyzing a reasonably small number of genes, patients with adenocarcinoma could be stratified according to their prognosis. The prognostic model could be applicable to future decisions concerning treatment.

Cited by 151 publications

References 32 publications

Prediction of recurrence in Ta urothelial cell carcinoma by real‐time quantitative PCR analysis: A microarray validation study

Prediction of recurrence in Ta urothelial cell carcinoma by real‐time quantitative PCR analysis: A microarray validation study

Oncogenic role of protein tyrosine kinase 7 in esophageal squamous cell carcinoma

Prognostic significance of Notch ligands in patients with non-small cell lung cancer

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