Prognostic significance of immunoglobulin heavy chain gene rearrangement in patients with acute myelogenous leukemia

Kyoda, Katsunori; Nakamura, Shinobu; Matano, Sadaya; Ohtake, Shigeki; Matsuda, Tamotsu

doi:10.1038/sj.leu.2400662

Cited by 31 publications

(28 citation statements)

References 3 publications

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“…The follow-up revealed that the complete remission rate and 2-year survival rate of the IgH rearrangement group were significantly lower than those of the negative IgH rearrangement group, indicating the IgH rearrangement may be a poor prognostic factor in AML, which was consistent with the results of Kyoda et al (1997). The significance of TCRγ rearrangement in AML has been little reported.…”

Section: Discussionsupporting

confidence: 79%

Significance of detecting IgH and TCRγ gene rearrangements in patients with hemopoietic maligancies by real-time quantitative PCR

Yao¹,

Rh²,

B³

et al. 2015

Genet. Mol. Res.

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Section: Discussionsupporting

confidence: 79%

Significance of detecting IgH and TCRγ gene rearrangements in patients with hemopoietic maligancies by real-time quantitative PCR

Yao¹,

Rh²,

B³

et al. 2015

Genet. Mol. Res.

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“…This finding is not surprising because in one study, 40% (of 35 cases) of human AML also exhibit this type of rearrangement, as well as expressing some B-cell-specific antigens. 10 In this case series, the presence of an IgH rearrangement was associated with poorer survival. In another study, T-or B-cell receptor rearrangements were found in 2 of 16 patients with myelodysplastic syndrome that progressed to myelogenous leukemia.…”

Section: Discussionmentioning

confidence: 92%

“…The detection limits of the assay are similar to that reported in human patients. 10 Sensitivity can be increased at least 10-fold if primers specific for the individual tumor, rather than consensus primers, are used. 22 Although not practical for routine clinical use, this method is frequently used experimentally for the detection of minimum residual disease after chemotherapy in human patients.…”

Section: Discussionmentioning

confidence: 99%

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Diagnosis of Canine Lymphoid Neoplasia Using Clonal Rearrangements of Antigen Receptor Genes

et al. 2003

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Abstract. Although the diagnosis of canine leukemia and lymphoma in advanced stages is usually uncomplicated, some presentations of the disease can be a diagnostic challenge. In certain situations, lymphoma and leukemia can be difficult to distinguish from a benign reactive proliferation of lymphocytes. Because clonality is the hallmark of malignancy, we have developed an assay that uses the polymerase chain reaction to amplify the variable regions of immunoglobulin genes and T-cell receptor genes to detect the presence of a clonal lymphocyte population. The assay detected clonally rearranged antigen receptor genes in 91% of the 77 dogs with lymphoid malignancy. Of the 24 dogs tested, that were either healthy or had clearly defined conditions not related to lymphoid malignancy, a clonally rearranged antigen receptor gene was found in one (a dog with Ehrlichia canis infection). Gene rearrangement was appropriate for the immunophenotype (immunoglobulin gene rearrangement in B-cell leukemias and T-cell receptor gene rearrangement in T-cell leukemias). Dilution analysis showed that the clonal rearrangement could be detected when 0.1-10% of the DNA was derived from neoplastic cells, depending on the source tissue. Potential applications of this assay include the diagnosis of lymphoma or leukemia in biopsy samples, cavity fluids, fine needle aspirates, bone marrow and peripheral blood; the determination of lineage (B or T cell); staging of lymphoma; and detection of residual disease after chemotherapy.Key words: Dogs; gene rearrangement; genes-immunoglobulin; leukemia; lymphoma; myeloma; receptorsantigen-T-cell.Detection of canine lymphocytic malignancies relies on the cytologic assessment of circulating lymphocytes or lymphoid tissue or on the histologic examination of the lymphoid tissue. The diagnosis is often straightforward but there are situations that present a diagnostic challenge. These include the early stages of lymphoma that may be difficult to distinguish from lymphoid hyperplasia; cavity fluids that contain large numbers of small, mature-appearing lymphocytes; cases with chronic or mild lymphocytosis; cases diagnosed by biopsy where the biopsy does not fully represent the lesion; and the finding of a small number of atypical lymphocytes in a fine needle aspirate or cavity fluid. In addition, mass aspirates can be limited by inadequate cell numbers or by aspirating a site that does not represent the lesion. Therefore, more sensitive and objective assays are desirable for detecting lymphoid malignancies in dogs.Because all lymphomas, lymphocytic leukemias, and myelomas are clonal expansions of lymphocytes, 1 Present address: Center for Cancer Causation and Prevention, AMC Cancer Research Center, Denver, CO. each particular neoplasm contains DNA regions that are unique in both length and sequence. The CDR3 region of both immunoglobulin and T-cell receptor (TCR) genes encodes the antigen-binding region of the respective receptor and contains the majority of this unique sequence. In B cells, CDR3 is produced ...

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“…28 The former explanation is supported by the observation that histiocytic neoplasms occasionally arise in association with mediastinal germ-cell tumors 81,82 and that IgH rearrangements have been previously reported in myeloid sarcoma. 83,84 Alternatively, cross-contamination from positive cases or 'pseudomonoclonality' due to extremely low numbers of lymphocytes may contribute to false positive molecular testing results. 85,86 Although lymphocytes were sparse in the specimens in the present study, duplicate PCR testing decreases the likelihood of this pitfall.…”

Section: Discussionmentioning

confidence: 99%

Histiocytic sarcoma: a study of five cases including the histiocyte marker CD163

et al. 2005

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Prognostic significance of immunoglobulin heavy chain gene rearrangement in patients with acute myelogenous leukemia

Cited by 31 publications

References 3 publications

Significance of detecting IgH and TCRγ gene rearrangements in patients with hemopoietic maligancies by real-time quantitative PCR

Significance of detecting IgH and TCRγ gene rearrangements in patients with hemopoietic maligancies by real-time quantitative PCR

Diagnosis of Canine Lymphoid Neoplasia Using Clonal Rearrangements of Antigen Receptor Genes

Histiocytic sarcoma: a study of five cases including the histiocyte marker CD163

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