Prognostic Significance of p27 and Survivin in H. pylori Gastritis and Gastric Cancer

Helal, Noha; Omran, Zeinab; Aboushousha, Tarek; Youssef, Magdy M.; Badawy, Ahmed; Aboul-Ezz, Mohammed; Moussa, Mona

doi:10.31557/apjcp.2021.22.11.3553

Cited by 4 publications

(9 citation statements)

References 25 publications

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“…The p27 protein (often referred to as KIP1) is a member of the cyclindependent kinase inhibitor (CDKI) KIP family of tumorsuppressor proteins regulated by transforming growth factor beta (TGF-β) [20]. One proposed mechanism for the development of cancer is the inactivation of this p27 KIP1 tumor suppressor gene, located on chromosome 12p13 through transcriptional regulation [23,24]. Eukaryotic cells have a network of regulatory proteins that affect cell cycle control, which regulate and control the cell cycle to prevent malignant cell proliferation and cancer [23].…”

Section: Helicobacter Pylorimentioning

confidence: 99%

A Genetic and Immunohistochemical Analysis of Helicobacter pylori Phenotypes and p27 Expression in Adenocarcinoma Patients in Jordan

Al-Sheboul

Mohammad

Shboul

et al. 2023

J Epidemiol Glob Health

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Stomach (gastric) cancer is one of the most prevalent and deadly cancers worldwide and most gastric cancers are adenocarcinomas. Based on prior research, there is an association between Helicobacter pylori (H. pylori) infection together with the frequency of duodenal ulcer, distal gastric adenocarcinoma, mucosa-associated lymphoid tissue (MALT) lymphoma, and antral gastritis. Helicobacter pylori virulence and toxicity factors have been identified before that significantly influence the clinical outcomes of H. pylori infection and gastric adenocarcinoma. However, it remains unclear exactly how different strains of H. pylori affect gastric adenocarcinoma. Current research suggests this involves tumor suppressor genes, like p27 but also H. pylori toxic virulence proteins. Therefore, we quantified known H. pylori genotypes within adenocarcinoma patients to establish the prevalence of known toxins that include cytotoxin-associated gene A (cagA) as well as vacuolating cytotoxin A (vacA) within patients of variable adenocarcinoma diagnosis. This analysis used gastrectomy samples validated for DNA viability. The incidence of H. pylori in adenocarcinoma patients in Jordan was established to be 54.5% positive (ureA gene positive) with cagA genotype occurrence at 57.1%, but also in this population study vacA gene ratios found to be 24.7%:22.1%:14.3%:14.3%. (vacAs1:vacAs2:vacAm1:vacAm2). Using immunohistochemistry (IHC), we confirmed with statistical significance that p27 was dysregulated and suppressed, within nearly all H. pylori vacA genotypes. In addition, within 24.6% of H. pylori samples analyzed was a different bacterial genotype, and curiously that p27 protein expression was retained in 12% of tested adenocarcinoma H. pylori samples. This is suggestive that p27 could be used as a prognostic indicator but also that an unknown genotype could be contributing to the regulatory effects of p27 protein within this bacterial and cellular environment that may include other virulence factors and unknown immune system regulatory changes.

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Section: Helicobacter Pylorimentioning

confidence: 99%

A Genetic and Immunohistochemical Analysis of Helicobacter pylori Phenotypes and p27 Expression in Adenocarcinoma Patients in Jordan

Al-Sheboul

Mohammad

Shboul

et al. 2023

J Epidemiol Glob Health

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“…Gastrointestinal cancers, including colorectal and stomach cancers, are the most common cancers (1). It takes years for the early changes in stomach cancer to turn into invasive cancer (2).…”

Section: Introductionmentioning

confidence: 99%

“…Early detection of gastric cancer in the early stages and identifying factors affecting the prognosis improve patients' quality of life (1). One of the issues emphasized today in the prediction of gastrointestinal cancers is the use of immunohistochemical tests to identify various molecules such as cytokeratins and tumor markers (12).…”

Section: Introductionmentioning

confidence: 99%

Determining the relationship between cytokeratin expression and prognostic factors in human gastric cancer

Zheng

Chen

Liu

et al. 2022

Cell Mol Biol (Noisy-le-grand)

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Determining the prognosis of gastric cancer is the most crucial step in the treatment process. Cytokeratins are intermediate filaments found in the intracellular structure of epithelial tissues. Recent researches have focused on determining the relationship between the expression of cytokeratins and the degree and prognosis of tumors. This study aimed to investigate the relationship between the incidence of cytokeratin-20 and cytokeratin-7 in patients with gastric carcinoma with factors influencing the prognosis. In this regard, the study was conducted cross-sectional. The expression of cytokeratin-20 and cytokeratin-7 was evaluated on 50 gastric adenocarcinoma specimens with different degrees of differentiation by the immunohistochemical method. We determined the relationship between the incidence of cytokeratin-20 and cytokeratin-7 with factors affecting the prognosis of patients, including the degree of differentiation of gastric cancer tissue, lymph node involvement, and the depth of tumor invasion. Data were statistically analyzed by Chi-square and Spearman tests. The results showed a statistically inverse relationship between the incidence of cytokeratin-20 and cytokeratin-7 with the degree of tissue differentiation and lymph node involvement in gastric cancer. Although there was a statistically significant relationship between the incidence of tissue invasion in gastric cancer and the incidence of cytokeratin-7, there was no association between the incidence of cytokeratin-20 and tissue invasion. In general, decreased cytokeratin-20 and cytokeratin-7 are associated with decreased tissue differentiation and increased lymph node involvement.

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Section: Introductionmentioning

confidence: 99%

“…The mechanism of the development of gastric adenocarcinoma in H. pylori -infected samples has not yet been determined [16]. One proposed mechanism for the development of cancer is the inactivation of the p27 KIP1 tumor suppressor gene, located on chromosome 12p13 [17,18]. Eukaryotic cells have a network of regulatory proteins that affect cell cycle control, which precisely regulate and control the progression of the cell through the cell cycle [17].…”

Section: Introductionmentioning

confidence: 99%

A Genetic and Immunohistochemical Analysis ofH. PyloriPhenotypes and p27 expression in Adenocarcinoma Patients in Jordan

Brown

Mohammad

Shboul

et al. 2022

Preprint

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Gastric cancer is one of the most prevalent malignant cancers worldwide and specifically, adenocarcinoma. Based on prior research, there is an association between Helicobacter pylori (H. pylori) infection and the frequency of duodenal ulcer, distal gastric adenocarcinoma, mucosa-associated lymphoid tissue lymphoma, and antral gastritis. H. pylori virulence and toxicity factors have been identified that significantly influence the clinical outcomes of H. pylori infection and gastric adenocarcinoma. However, it is unclear exactly how different strains of H. pylori infection affect gastric adenocarcinoma. Current research suggests this involves tumor suppressor genes, like p27, but also H. Pylori toxic proteins. Therefore, we quantified known H. Pylori genotypes within adenocarcinoma patients to establish the prevalence of known toxins that include cytotoxin-associated gene A (cagA) and vacuolating cytotoxin A (vacA) within patients of variable diagnosis. This analysis used gastrectomy samples which were validated for DNA viability. The incidence of H. Pylori in adenocarcinoma patients in Jordan was established to be 54.5% positive (ureA gene) with cagA genotype occurrence at 57.1% but also vacA gene ratios 24.7%:22.1%: 14.3%:14.3%. (vacAs1:vacAs2: vacAm1:vacAm2). It is statistically significant that p27 was dysregulated and suppressed within nearly all H. Pylori vacA genotypes but also that according to our analysis that 24.6% of H. Pylori samples analyzed had an unknown novel genotype and curiously that p27 protein expression was retained in only 12% of tested adenocarcinoma H. Pylori samples which is suggestive that p27 could be used as a prognostic indicator but also that an un-known yet genotype as yet not characterized could be contributing to the regulation of p27 protein within this cellular environment.

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Prognostic Significance of p27 and Survivin in H. pylori Gastritis and Gastric Cancer

Cited by 4 publications

References 25 publications

A Genetic and Immunohistochemical Analysis of Helicobacter pylori Phenotypes and p27 Expression in Adenocarcinoma Patients in Jordan

A Genetic and Immunohistochemical Analysis of Helicobacter pylori Phenotypes and p27 Expression in Adenocarcinoma Patients in Jordan

Determining the relationship between cytokeratin expression and prognostic factors in human gastric cancer

A Genetic and Immunohistochemical Analysis ofH. PyloriPhenotypes and p27 expression in Adenocarcinoma Patients in Jordan

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