Prognostic value of a pre-freeze hypo-osmotic swelling test on the post-thaw quality of dog semen

Karger, S; Geiser, B; Grau, Manuel; Burfeind, O.; Heuwieser, W.; Arlt, Sebastian

doi:10.1016/j.anireprosci.2016.01.015

Cited by 10 publications

(9 citation statements)

References 22 publications

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“…The progressive motility examination of Kintamani dog semen showed 87%. Previous studies by Rijsselaere et al (2011), Domosławska et al (2013), Jarosz et al (2016), and Karger et al (2016) showed 80, 85.31±8.8, 70.6±10.2, and 83%, respectively. Meanwhile, from the viability of the Kintamani dog's semen with eosin-nigrosine staining, the live sperm were transparent, and the dead were stained red, as shown in Fig 1 . The results indicate 92%, while other studies reported 90% (Rijsselaere et al 2011).…”

Section: Characteristics Of Kintamani Dog Fresh Semenmentioning

confidence: 73%

“…The abnormal semen examination of Kintamani dogs yielded 7% abnormal forms, as shown in Fig. 2, 3, and 4, while other studies reported 10% (Rijsselaere et al 2011) and 9-18% (Karger et al 2016). Furthermore, the analysis of the semen on intact plasma membrane was 89%, while other studies had 91% (87-96) (Karger et al 2016).…”

Section: Characteristics Of Kintamani Dog Fresh Semenmentioning

confidence: 83%

“…2, 3, and 4, while other studies reported 10% (Rijsselaere et al 2011) and 9-18% (Karger et al 2016). Furthermore, the analysis of the semen on intact plasma membrane was 89%, while other studies had 91% (87-96) (Karger et al 2016). While, the sperm with a good intact plasma membrane is shown in Fig.…”

Section: Characteristics Of Kintamani Dog Fresh Semenmentioning

confidence: 83%

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Spermatozoa Quality of Kintamani Dogs in Coconut Water-Egg Yolk Diluent with Addition of Moringa Leaves and Carrot Extract

2022

Int J Vet Sci

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The success of artificial insemination in dogs is significantly influenced by the quality of the semen used. Furthermore, during storage at 5oC, the semen is attacked by free radicals, which adversely affect its quality. Moringa leaves, and carrot extracts are natural ingredients with high antioxidant content, expected to overcome free radical attacks and oxidative stress experienced by sperm during storage. Therefore, this study aims were to examine the effect of adding Moringa leaf extract or carrot juice in coconut water-egg yolk diluent on the sperm quality of Kintamani dogs. The method used a completely random design. Three treatment groups exist for each diluent. The base diluent consists of young coconut water-egg yolk (D1), the base diluent plus moringa leaves extract (D2), and the base diluent plus carrot juice (D3). The life spermatozoa were observed in eight sections, from 0 (control) to 84 hours with 12 hourly observations. Each treatment group was examined for spermatozoa progressive motility, viability, abnormalities, and intact plasma membrane. The results showed that D2 maintained semen quality significantly better (P<0.05) than D1 and D3. However, D3 was better than D1 (P<0.05). It was concluded that the addition of moringa leaves extract in coconut water-egg yolk diluent maintained the best quality of spermatozoa for 60 hours, with motility, viability, abnormality, and intact plasma membrane of 56.67±19.98, 60.83±18.63, 11.83±2, 64, and 55.83±17.45%, respectively.

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Section: Characteristics Of Kintamani Dog Fresh Semenmentioning

confidence: 73%

Section: Characteristics Of Kintamani Dog Fresh Semenmentioning

confidence: 83%

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Spermatozoa Quality of Kintamani Dogs in Coconut Water-Egg Yolk Diluent with Addition of Moringa Leaves and Carrot Extract

2022

Int J Vet Sci

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“…Figura 1 -citologia vaginal avaliada em microscopia eletrônica: fase de estro O sêmen foi coletado pelo método de manipulação digital (Setyawan et al, 2016;Karger et al, 2016) e como reprodutor foi selecionado um cão adulto e sadio da raça Bull Terrier, que já teve atividade reprodutiva anteriormente por duas vezes, por monta natural (Figura 2).…”

Section: Relato De Casounclassified

Inseminação artificial e acompanhamento da gestação em uma cadela da raça Bull Terrier com histórico de agressividade

et al. 2020

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O objetivo desse trabalho é relatar uma inseminação artificial (IA) e posterior acompanhamento gestacional realizados em uma cadela da raça Bull Terrier com histórico de agressividade durante o estro. Foi atendida uma cadela da raça Bull Terrier com 14 meses de idade com sinais aparentes de estro, que demonstrou agressividade e intolerância à aproximação do macho na monta natural, sendo sugerido realizar a IA. A citologia vaginal confirmou a fase de estro, com mais de 80% de células queratinizadas anucleadas. O sêmen do macho doador foi coletado por manipulação digital e posteriormente analisado, apresentando qualidade em todos os parâmetros espermáticos avaliados. Foi realizada inseminação artificial intravaginal com sêmen fresco, e o mesmo procedimento foi repetido 48 horas, depois de uma segunda citologia vaginal confirmando que o animal ainda estava em estro. Essa repetição da IA teve o intuito de abranger melhor a duração do estro, aumentando a chance de prenhês. Após 51 dias da última IA foi realizada uma ultrassonografia para confirmar a gestação, sendo possível observar a presença de fetos vivos, cuja viabilidade foi comprovada pela presença de batimentos cardíacos entre 230 a 257 bpm. O parto ocorreu 58 dias após a última inseminação, com duração de aproximadamente oito horas e nascimento de cinco filhotes, todos vivos e saudáveis. Foi possível concluir que IA após confirmação de estro por citologia vaginal, e o acompanhamento gestacional, são ferramentas importantes da biotecnologia da reprodução, capazes de proporcionar a utilização de animais na atividade reprodutiva, em casos onde a monta natural não é possível.

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“…On the one hand, many studies proved its correlation with other sperm characteristics [ 3 , 4 , 5 , 6 , 7 ], as well as sperm fertilizing ability assessed in vitro and in vivo [ 4 , 5 , 8 , 9 ] and semen freezability [ 3 , 5 ]. On the other hand, some authors did not confirm these findings [ 10 , 11 ], which may be the result of species-specific differences and the lack of standardization of this test for particular species [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Hypo-Osmotic Swelling Test (HOST) for Feline Spermatozoa: The Simplified Procedure and the Aspect of Sperm Morphology

Prochowska

Niżański

Fontbonne

2022

Animals

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Hypo-osmotic swelling test (HOST) is used to assess the functional integrity of sperm plasma membranes in many species. The primary aim of this study was to test a simplified HOST procedure for the evaluation of feline semen. The second objective was to check if sperm abnormalities can influence the results of this test. Urethral semen was collected from 19 male, domestic cats. In Exp. 1, HOST was performed in different media (50 mOsm/kg fructose or distilled water), temperature (37 °C or room temperature) and time (5 and 30 min). In Exp. 2, the potential effect of sperm abnormalities on HOST results was assessed by observing individual normal and abnormal spermatozoa microinjected into droplets of distilled water. The results showed no differences between the HOST results performed in different media, temperature and time. Viable abnormal spermatozoa were able to swell under hypo-osmotic conditions in the same manner as normal ones, except spermatozoa with distal droplets, which showed a higher frequency of ‘despiralization’. In conclusion, HOST can be reliably performed at 0 mOsm/kg for 5 min at room temperature, which may contribute to a wider use of this test under clinical environments. Viable abnormal spermatozoa are able to swell under hypo-osmotic conditions; therefore, their presence in the ejaculate would not bias the results of HOST when total coiling is calculated.

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Prognostic value of a pre-freeze hypo-osmotic swelling test on the post-thaw quality of dog semen

Cited by 10 publications

References 22 publications

Spermatozoa Quality of Kintamani Dogs in Coconut Water-Egg Yolk Diluent with Addition of Moringa Leaves and Carrot Extract

Spermatozoa Quality of Kintamani Dogs in Coconut Water-Egg Yolk Diluent with Addition of Moringa Leaves and Carrot Extract

Inseminação artificial e acompanhamento da gestação em uma cadela da raça Bull Terrier com histórico de agressividade

Hypo-Osmotic Swelling Test (HOST) for Feline Spermatozoa: The Simplified Procedure and the Aspect of Sperm Morphology

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