Prognostic Value of MicroRNA-125 in Various Human Malignant Neoplasms: a Meta-Analysis

Sun, Xiaoxi; Zhang, Shanshan; Ma, Xiaoling

doi:10.7754/clin.lab.2015.150408

Cited by 17 publications

(17 citation statements)

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“…Of these 69 miRs there was a greater than 2-fold increase in the expression level of miR-125b-5p, miR-128-3p, miR-21-5p, miR-100-5p, miR-140-3p and miR-374b-5p, and a greater than 2-fold decrease in let-7d, miR-103-3p, miR-30a-5p and miR-27b-3p. The majority of miRs with significant expression differences have been previously reported as potential regulators and biomarkers in IPF progression or IPF-associated carcinogenesis (16)(17)(18)(19)(20)(21)(22)(23), among which miR-30a-5p demonstrated the most significance and was selected for further investigation in the present study (P<0.001).…”

Section: Characteristics Of Patients With Ipf and Healthy Participantsmentioning

confidence: 99%

Downregulation of microRNA‑30a in bronchoalveolar lavage fluid from idiopathic pulmonary fibrosis patients

Liu

Jiang

et al. 2018

Mol Med Report

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MicroRNAs (miRs) are short, highly conserved small noncoding RNA molecules with fundamental roles in regulating gene expression. To identify miR biomarkers associated with idiopathic pulmonary fibrosis (IPF), the expression pattern of miRs in exosomes from bronchoalveolar lavage fluid (BALF) of elderly patients with IPF were evaluated. High-throughput quantitative detection of miR expression using a microarray indicated that miR-125b, miR-128, miR-21, miR-100, miR-140-3p and miR-374b were upregulated in patients with IPF, while let-7d, miR-103, miR-26 and miR-30a-5p were downregulated. The expression level of miR-30a-5p was further examined, and its potential target genes were predicted using target gene prediction analysis software. A direct regulatory association was confirmed between miR-30a-5p and TGF-β activated kinase 1/MAP3K7 binding protein 3 (TAB3) via a dual-luciferase reporter assay. Overexpression of miR-30a-5p decreased TAB3, α-smooth muscle actin and fibronectin expression in A549 cells with or without transforming growth factor-β1 treatment. The decreased expression of miR-30a in the BALF of patients with IPF, along with the consequential increase in TAB3 expression, may be a crucial factor in IPF progression.

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Section: Characteristics Of Patients With Ipf and Healthy Participantsmentioning

confidence: 99%

Downregulation of microRNA‑30a in bronchoalveolar lavage fluid from idiopathic pulmonary fibrosis patients

Liu

Jiang

et al. 2018

Mol Med Report

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“…The expression patterns of many miRNAs are altered in hepatoblastoma patients, and this alteration plays a crucial role in HB pathogenesis [25, 26]. The miRNA-125 family (miRNA-125a-3p, miRNA-125a-5p, miRNA-125b-1, and miRNA-125b-2) is highly-conserved in many species, and expression of these miRNAs regulates carcinogenesis and tumor development by targeting many tumor-promoting and -suppressing transcription factors [27, 28]. Specifically, the inhibitory effects of miR125b on human cancer cell proliferation and metastasis have been extensively investigated [29].…”

Section: Introductionmentioning

confidence: 99%

GATA4 promotes hepatoblastoma cell proliferation by altering expression of miR125b and DKK3

et al. 2016

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GATA4 is a zinc finger DNA-binding protein that plays an important role in mammalian liver development. However, the effects of GATA4 in hepatoblastoma (HB), a common liver cancer in pediatric patients, remain largely unknown. In this study, we demonstrate that GATA4 promotes growth and survival in the Huh6 human hepatoblastoma cell line. GATA4 expression was high in Huh6 cells, and its knockdown decreased expression of Dickkopf-related protein 3 (DKK3), a gene that may contribute to premature or undifferentiated phenotypes in HB. GATA4 also directly bound to the promoter regions of the miRNA miR125b and inhibited its expression in Huh6 cells. DKK3 was a direct target of miR125b in Huh6 cells. Inhibition of miR125b or overexpression of DKK3 promoted proliferation, survival, migration, and invasion in Huh6 cells. This is the first report to demonstrate that GATA4 promotes oncogenesis by inhibiting miR125b-dependent suppression of DKK3 expression. This GATA4/miR125b/DKK3 axis may be a major regulator of growth, migration, invasion, and survival in hepatoma cells, and is therefore a potential therapeutic target or biomarker for progression in HB patients.

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“…Higher expression of miR-125a may predict better survival in NSCLC patients. 14,[34][35][36][37] Wang et al reported that miR-125a, as a metastatic suppressor in lung cancer cells, activated by epidermal growth factor receptor (EGFR) signaling, inhibits tumorigenesis and tube formation. 38 In our study, rs8111742 located 1033bp upstream of miR-125a was associated with better survival in NSCLC patients.…”

Section: F I G U R E 1 Kaplan-meier Plots Of Survival According Tomentioning

confidence: 99%

Genetic variations in miR‐125 family and the survival of non‐small cell lung cancer in Chinese population

Shen

Yang

et al. 2019

Cancer Medicine

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To investigate the associations between the functional single nucleotide polymorphisms (SNPs) in the miR‐125 family and the survival of non‐small cell lung cancer (NSCLC) patients, we systematically selected six functional SNPs located in three pre‐miRNAs (miR‐125a, miR‐125b‐1, miR‐125b‐2). Cox proportional hazard regression analyses were conducted to estimate the crude and adjusted hazard ratios (HRs) and their 95% confidence intervals (CIs). Reporter gene luciferase assay was performed to examine the relationship between the SNPs and transcriptive activity of the miRNAs. The expression of miRNAs in different cells was detected using quantitative real‐time PCR assay. We found that rs2241490 (upstream of miR‐125b‐1, G > A, adjusted HR = 1.24, 95%CI = 1.05‐1.48, P = 0.014, in dominant model; adjusted HR = 1.18, 95%CI = 1.03‐1.35, P = 0.014, in additive model), rs512932 (upstream of miR‐125b‐1, A > G, dominant model: adjusted HR = 1.25, 95%CI = 1.05‐1.48, P = 0.013) and rs8111742 (upstream of miR‐125a, G > A, dominant model: adjusted HR = 0.84, 95%CI = 0.71‐1.00, P = 0.047) were associated with the prognosis of 1001 Chinese NSCLC patients. The combined analysis of the three SNPs related the number of risk alleles (rs2241490‐A, rs512932‐G and rs8111742‐G) to death risk of NSCLC in a locus‐dosage mode ( P for trend <0.001). Furthermore, luciferase reporter gene assay showed significantly higher levels of luciferase activity with rs512932 variant G than that with A allele in 293T, SPC‐A1 and A549 cell lines. Besides, miR‐125b was highly expressed in lung cancer cells than the normal lung cell. Our study indicated that genetic variations in miR‐125 family were implicated in the survival of NSCLC patients. Larger population‐based and functional studies are needed to verify these findings.

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Prognostic Value of MicroRNA-125 in Various Human Malignant Neoplasms: a Meta-Analysis

Cited by 17 publications

References 0 publications

Downregulation of microRNA‑30a in bronchoalveolar lavage fluid from idiopathic pulmonary fibrosis patients

Downregulation of microRNA‑30a in bronchoalveolar lavage fluid from idiopathic pulmonary fibrosis patients

GATA4 promotes hepatoblastoma cell proliferation by altering expression of miR125b and DKK3

Genetic variations in miR‐125 family and the survival of non‐small cell lung cancer in Chinese population

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