Prognostic Value of Serum Sialyl Lewis^a, Sialyl Lewis^x and Sialyl Tn Antigens in Blood from the Tumor Drainage Vein of Colorectal Cancer Patients

Abstract

The serum levels of sialyl Lewis^a (CA19-9), sialyl Lewis^x (SLX), sialyl Tn (STN) and carcinoembryonic antigen (CEA) in peripheral venous blood and tumor drainage venous blood of colorectal cancer patients were examined to determine their value as prognostic factors after surgery. Blood samples were obtained from the peripheral vein and from the tumor drainage vein from 124 colorectal cancer patients during surgical excision of the tumor. The patients were divided into high-antigen and low-antigen groups for each antigen in each location. Serum levels of SLX, STN and CEA in tumor drainage venous blood (d-SLX, d-STN and d-CEA, respectively) were significantly higher than in peripheral venous blood (p-SLX, p-STN and p-CEA, respectively). The survival time after surgery for patients with high d-SLX or d-CEA levels and low levels of the same antigen in peripheral venous blood was significantly shorter than the survival time for those patients with low levels of antigen at both sites (p = 0.0003 or p = 0.0406, respectively). Cox’s regression analysis revealed that a high d-SLX or high d-CEA level was an independent prognostic variable for patient survival. In conclusion, determining d-SLX or d-CEA is more useful than p-SLX or p-CEA in predicting the outcome for colorectal cancer patients.

E-Cadherin truncation and Sialyl Lewis-X overexpression in oral squamous cell carcinoma and oral precancerous conditions

“…The presence of carbohydrate receptor sLe X in tumors or serum has been associated well with poor prognosis of various cancers [12,14,16,17] and suggested no other carbohydrate epitope examined to date has equal prognostic value. sLe X expressions in colorectal and non-small cell lung cancer has been correlated with advanced disease, increased distant metastasis and decreased survival [41].…”

“…sLe X is frequently expressed on human cancer cells; serve as ligand for E-and P-selectin present on vascular endothelial cells [11,15]. The degree of expression of this ligand at the cancer cells surface is thought to be correlated with the frequency of hematogenous metastasis and prognostic outcome of cancer patients [16][17][18]. It has been shown that expression of sLe X in mucin-type O-glycans is highly associated with lymphatic and venous invasion [19].…”

Comparative Epitope Mapping with Saturation Transfer Difference NMR of Sialyl Lewisa Compounds and Derivatives Bound to a Monoclonal Antibody

“…5,10,11 The monoclonal antibody GSLA-2 detects the tumor-associated antigen CA19-9, [12][13][14][15] which comprises the sLe a epitope in sera from patients with pancreatic and gastrointestinal cancer. 16,17 An automated cancer diagnostic assay based on GSLA-2 has a proven clinical performance and demonstrates good correlation with the approved CA19-9 assay. 18 The sLe a epitope is characterized by a fucose and a sialic acid residue attached to the type 2 LacNAc core, whereas the sLe x epitope contains the type 1 core structure.…”

Colorectal cancer metastasis: in the surgeon's hands?

“…Fujita et al [52] found patients negative preoperatively for CK-19 or CK-20 had significantly fewer recurrences and better 5-year disease free survival. Nakagoe et al [53] found a high sialyl Le (x) antigen or CEA in blood draining the tumour was an independent prognostic indicator of poor survival, and Yamaguchi et al [12] found a similar finding in patients positive for both CEA and CK-20. Other studies show conflicting results, however.…”