Prognostic value of soluble H7-B4 in pleural effusion associated with lung cancer

Abstract

B7-H4, a member of the inhibitory B7 family, can restrain T cell proliferation, activation, and cytokine secretion and may be involved in immune evasion in cancer patients. This study aimed to evaluate the diagnostic and prognostic value of pleural effusion levels of soluble B7-H4 (sB7-H4) in lung cancer patients with malignant pleural effusion (MPE). Pleural effusion samples were collected from 98 lung cancer patients with malignant effusion and from 60 patients with nonmalignant pleural effusion. Pleural effusion concentrations of sB7-H4 were measured using sandwich enzyme-linked immunosorbent assay. Malignant effusion exhibited higher sB7-H4 levels than those in nonmalignant effusion (P < 0.01). Lung cancer patients with pleural effusion sB7-H4 levels below 35.8 ng/ml had a longer overall survival than those with higher levels (P < 0.05). By multivariate analysis, pleural effusion sB7-H4 was an independent prognostic factor in patients with MPE. In conclusion, measurement of sB7-H4 might be a useful diagnostic and prognostic value for MPE patients.

Diagnostic value of soluble B7‐H4 and carcinoembryonic antigen in distinguishing malignant from benign pleural effusion

“…In our study, we obtained an optimized cutoff value of sB7‐H4 36.5 ng/ml and CEA 4.18 ng/ml to differentiate MPE from BPE with a corresponding sensitivity (81.82%, 87.28%) and specificity (90.48%, 95.24%). This was consistent with the report by Xu et al, in which exhibited a sensitivity of 78.6% and a specificity of 91.7% of sB7‐H4 for MPE associated with lung cancer with a cutoff point 35.8 ng/ml.…”

“…B7‐H4, which belongs to a novel member of the inhibitory B7 family, plays an important role in the process of modulating the tumor cellular immune response . Previous studies had confirmed that B7‐H4 overexpressed in various human malignant tumors including non‐small‐cell lung cancer, breast, esophageal, gastric, bladder, osteosarcoma, hepatocellular, and so on, which were notably related to tumor malignant degree, progression, and prognosis, while not expressed or down‐regulated in normal human tissues . In recent years, sB7‐H4 was regarded as a potentially valuable tumor marker .…”

“…Then, it was centrifuged with 4800 rpm at −4°C for 15 min, and the extracted supernatant was stored at −70°C. Sandwich ELISA with the commercial sB7‐H4 ELISA Ready‐SET‐Go kit (eBioscience, San Diego) was applied to determinate the sB7‐H4 level of pleural effusion . CEA concentration in pleural effusion was detected by electro‐chemiluminescence immunoassays on Roche Cobas e601 analyzer.…”

Soluble B7-CD28 Family Inhibitory Immune Checkpoint Proteins and Anti-Cancer Immunotherapy

“…Additionally, sB7-H4 was also correlated with serum AFP levels in HCC, distant metastasis at nephrectomy in RCC, distant metastasis in osteosarcoma, and histology type in ovarian cancer ( 247 , 248 , 250 , 252 , 254 ). Soluble B7-H4 levels in malignant pleural effusion were higher in lung cancer and predicted a worst prognosis ( 255 ). Elevated preoperative levels had predicted a worse response to anti-VEGF therapy in RCC ( 250 ).…”

The tissue distribution and significance of B7-H4 in laryngeal carcinoma

“…It has also been reported that the expression of B7-H4 is associated with TNM staging and the pathological grading of bladder cancer and thyroid cancer [ 29 , 30 ]. Additionally, soluble B7-H4 can be used as a diagnostic and prognostic marker of malignant pleural effusion [ 31 ]. However, the expression and the potential role of B7-H4 in LC development have not been reported.…”