Prognostic value of type XXII and XXIV collagen mRNA expression in head and neck cancer patients

Abstract

Abstract. Squamous cell carcinoma of the head and neck (HNSCC) is the sixth most common type of cancer, affecting ~500,000 individuals worldwide annually. Collagen is the major constituent of the extracellular matrix component in tumors and plays a crucial role in tumor development. The aim of this study was to determine the mRNA expression of type XXI, XXII, XXIII and XXIV α1 collagen (COL21A1, COL22A1, COL23A1 and COL24A1, respectively) in head and neck squamous cell carcinoma (HNSCC) and investigate its correlation with disease progression. This study investigated the mRNA expression levels of COL21A1, COL22A1, COL23A1 and COL24A1 in 70 HNSCC primary samples and 44 matched pairs of tumor and adjacent normal mucosal tissues using quantitative polymerase chain reaction (qPCR). Expression data were compared to the clinicopathological variables in order to determine the correlation between expression and disease progression. Our results demonstrated that the mRNA levels of COL22A1 and COL24A1 were significantly higher in HNSCC tissues compared to those in the corresponding normal tissues from the same individuals (n=44; P<0.001 and P=0.019, respectively). The COL22A1 mRNA levels were found to be significantly associated with lymph node metastasis (P=0.018) and pathological stage (P=0.024), whereas the COL24A1 mRNA levels were significantly associated with tumor size (P=0.045). The high expression levels of COL22A1 and COL24A1 mRNA were statistically correlated with a decrease in disease-free survival (DFS) (log-rank test, P<0.001). The results of the multivariate logistic regression analysis revealed that high expression levels of the COL22A1 and COL24A1 gene pair were associated with a high odds ratio for recurrence of 14.62 (95% confidence interval: 2.77-77.26; P=0.002). Therefore, the upregulation of COL22A1 and COL24A1 mRNA may play a critical role in the progression of HNSCC and provide useful information as a prognostic predictor for HNSCC patients.

Curcuminoids combined with gefitinib mediated apoptosis and autophagy of human oral cancer SAS cells in vitro and reduced tumor of SAS cell xenograft mice in vivo

“…Head and neck carcinoma is the sixth most common cancer worldwide and about 95% of them are squamous cell carcinoma (SCC) which is still a significant cause of death in the world and this disease affects nearly 500,000 patients annually worldwide . The possible factors for causing oral cancer include environment, nutritional deficiencies, consumptions of alcoholic products, tobacco smoking, betel quid chewing, and papillomaviruses infection .…”

Extracellular matrix macromolecules: potential tools and targets in cancer gene therapy

“…On the other hand, degradation of collagen type XVIII resulting in the formation of endostatin causes inhibition of angiogenesis and thereby retards tumour growth [ 43 ]. Furthermore, some collagens such as type XXII and XXIV have been referred to have prognostic value in certain cancers [ 44 ].…”

<p>Overexpression of COL24A1 in Hepatocellular Carcinoma Predicts Poor Prognosis: A Study Based on Multiple Databases, Clinical Samples and Cell Lines</p>

“…5,6 Collagen is also the main component of the tumor extracellular matrix, and it plays an important role in the development of tumors. 7,8 Many studies have investigated the collagen family in HCC. Hepatocellular carcinoma is primarily associated with cirrhosis.…”

“…15 In addition, Dr. Kiyoshi has demonstrated that the overexpression of the COL24A1 gene may be a valuable biomarker for prognosis in HNSCC. 7 Certain markers, such as AFP, 16 AFP-L3, 17 GCP3, 18 and MDK, 19 have often been used in HCC diagnosis. Nevertheless, most of these markers are unlikely to exactly reflect the diagnostic and therapeutic efficiency.…”