Programmable Paper-Based Microfluidic Devices for Biomarker Detections

Soum, Veasna; Park, Sooyong; Brilian, Albertus Ivan; Kwon, Oh‐Sun; Shin, Kwanwoo

doi:10.3390/mi10080516

Cited by 71 publications

(58 citation statements)

References 109 publications

(191 reference statements)

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“…Apart from LFIA, paper-based microuidic devices have been experiencing rapid development in recent years, and their feasibility in portable detection of muscle injuries has been unfolded. 15,[66][67][68][69][70] For example, Gao et al developed a vertical paper-based analytical devices that realized automated ELISA for Myo detection for muscle injury diagnostics. 71 In this design, the intensity of color produced in the reaction area is used as a metric to evaluate Myo concentrations with a limit of detection of 32 ng mL À1 .…”

Section: Portable Devices For Biomedical Analysismentioning

confidence: 99%

Diagnosis and prognosis for exercise-induced muscle injuries: from conventional imaging to emerging point-of-care testing

Tang

Liu

et al. 2020

RSC Adv.

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Section: Portable Devices For Biomedical Analysismentioning

confidence: 99%

Diagnosis and prognosis for exercise-induced muscle injuries: from conventional imaging to emerging point-of-care testing

Tang

Liu

et al. 2020

RSC Adv.

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“…The capillary is the driving force for fluid to move into the tube, and the velocity of the fluid is reduced as the fluid penetrates into the capillary. More detailed discussions can be found in other reports [11,12,44,45]. Fluid manipulation technology can be divided into passive and active methods according to the operation modes.…”

Section: Introductionmentioning

confidence: 99%

Recent Advances of Fluid Manipulation Technologies in Microfluidic Paper-Based Analytical Devices (μPADs) toward Multi-Step Assays

Kim¹,

Hahn

Kim

2020

Micromachines

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Microfluidic paper-based analytical devices (μPADs) have been suggested as alternatives for developing countries with suboptimal medical conditions because of their low diagnostic cost, high portability, and disposable characteristics. Recently, paper-based diagnostic devices enabling multi-step assays have been drawing attention, as they allow complicated tests, such as enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR), which were previously only conducted in the laboratory, to be performed on-site. In addition, user convenience and price of paper-based diagnostic devices are other competitive points over other point-of-care testing (POCT) devices, which are more critical in developing countries. Fluid manipulation technologies in paper play a key role in realizing multi-step assays via μPADs, and the expansion of biochemical applications will provide developing countries with more medical benefits. Therefore, we herein aimed to investigate recent fluid manipulation technologies utilized in paper-based devices and to introduce various approaches adopting several principles to control fluids on papers. Fluid manipulation technologies are classified into passive and active methods. While passive valves are structurally simple and easy to fabricate, they are difficult to control in terms of flow at a specific spatiotemporal condition. On the contrary, active valves are more complicated and mostly require external systems, but they provide much freedom of fluid manipulation and programmable operation. Both technologies have been revolutionized in the way to compensate for their limitations, and their advances will lead to improved performance of μPADs, increasing the level of healthcare around the world.

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“…In response to the need of finding a low-cost and high-throughput fabrication method to produce miniaturized paper-based assays [37][38][39], we have developed an approach using a desktop pen plotter and technical pens ( Figure 1). A commercially available water-based hydrophobic solution with an added colorant for visualization of the printed pattern was used for plotting hydrophobic barriers.…”

Section: Introductionmentioning

confidence: 99%

Pushing the Limits of Spatial Assay Resolution for Paper-Based Microfluidics Using Low-Cost and High-Throughput Pen Plotter Approach

et al. 2020

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To transform from reactive to proactive healthcare, there is an increasing need for low-cost and portable assays to continuously perform health measurements. The paper-based analytical devices could be a potential fit for this need. To miniaturize the multiplex paper-based microfluidic analytical devices and minimize reagent use, a fabrication method with high resolution along with low fabrication cost should be developed. Here, we present an approach that uses a desktop pen plotter and a high-resolution technical pen for plotting high-resolution patterns to fabricate miniaturized paper-based microfluidic devices with hundreds of detection zones to conduct different assays. In order to create a functional multiplex paper-based analytical device, the hydrophobic solution is patterned on the cellulose paper and the reagents are deposited in the patterned detection zones using the technical pens. We demonstrated the effect of paper substrate thickness on the resolution of patterns by investigating the resolution of patterns on a chromatography paper with altered effective thickness. As the characteristics of the cellulose paper substrate such as thickness, resolution, and homogeneity of pore structure affect the obtained patterning resolution, we used regenerated cellulose paper to fabricate detection zones with a diameter as small as 0.8 mm. Moreover, in order to fabricate a miniaturized multiplex paper-based device, we optimized packing of the detection zones. We also showed the capability of the presented method for fabrication of 3D paper-based microfluidic devices with hundreds of detection zones for conducting colorimetric assays.

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Programmable Paper-Based Microfluidic Devices for Biomarker Detections

Cited by 71 publications

References 109 publications

Diagnosis and prognosis for exercise-induced muscle injuries: from conventional imaging to emerging point-of-care testing

Diagnosis and prognosis for exercise-induced muscle injuries: from conventional imaging to emerging point-of-care testing

Recent Advances of Fluid Manipulation Technologies in Microfluidic Paper-Based Analytical Devices (μPADs) toward Multi-Step Assays

Pushing the Limits of Spatial Assay Resolution for Paper-Based Microfluidics Using Low-Cost and High-Throughput Pen Plotter Approach

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