Programmed cell adhesion and growth on cell-imprinted polyacrylamide hydrogels

DePorter, Sandra M.; Lui, Irene; McNaughton, Brian R.

doi:10.1039/c2sm25622c

Cited by 35 publications

(34 citation statements)

References 43 publications

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“…This finding is particularly important, as polymerization conditions frequently involve high concentrations of cytotoxic monomers, organic solvents, UV irradiation or heat, each which would jeopardize the viability of live cultures [54]. In a separate study, DePorter et al imprinted HEK293, HeLa, and MRC-9 cell lines in poly(acrylamide) hydrogels [55]. While cells failed to adhere to non-imprinted poly(acrylamide), MIP poly(acrylamide) served as a suitable surface for the adhesion and proliferation of each cell lineage.…”

Section: Molecularly Imprinted Polymersmentioning

confidence: 99%

“…The authors posited, herein, that the cells’ topographical features, which were imparted by the imprinting protocol, promoted cell-selective adhesion to the MIP poly(acrylamide). Additional data presented in the manuscript also suggests that cellular fragments (such as peptides, proteins, lipids, and nucleic acids) remained in some of the MIP formulations, which could also contribute to subsequent cell adhesion [55]. …”

Section: Molecularly Imprinted Polymersmentioning

confidence: 99%

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Vision for Functionally Decorated and Molecularly Imprinted Polymers in Regenerative Engineering

Clegg

Wechsler

Peppas

2017

Regen. Eng. Transl. Med.

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The emerging field of regenerative engineering offers a great challenge and an even greater opportunity for materials scientists and engineers. How can we develop materials that are highly porous to permit cellular infiltration, yet possess sufficient mechanical integrity to mimic native tissues? How can we retain and deliver bioactive molecules to drive cell organization, proliferation, and differentiation in a predictable manner? In the following perspective, we highlight recent studies that have demonstrated the vital importance of each of these questions, as well as many others pertaining to scaffold development. We posit hybrid materials synthesized by molecular decoration and molecular imprinting as intelligent biomaterials for regenerative engineering applications. These materials have potential to present cell adhesion molecules and soluble growth factors with fine-tuned spatial and temporal control, in response to both cell-driven and external triggers. Future studies in this area will address a pertinent clinical need, expand the existing repertoire of medical materials, and improve the field’s understanding of how cells and materials respond to one another.

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Section: Molecularly Imprinted Polymersmentioning

confidence: 99%

Section: Molecularly Imprinted Polymersmentioning

confidence: 99%

Vision for Functionally Decorated and Molecularly Imprinted Polymers in Regenerative Engineering

Clegg

Wechsler

Peppas

2017

Regen. Eng. Transl. Med.

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“…[191,192] In addition, the availability of imprinting sites on the bacteria imprinting polymers (BIPs) is another critical factor for efficient bacterial imprinting. Surface imprinting, including several typical methods, such as sol-gel, lithog raphy, [193,194] microcontact imprinting, [195,196] Pickering emulsion, [197,198] and colloidal imprinting, [199,200] have been developed, which can ensure the effective diffusion of these large targets from the cavity.…”

Section: Bacteria Imprintingmentioning

confidence: 99%

Molecularly Imprinted Materials for Selective Biological Recognition

Zhang

et al. 2019

Macromol. Rapid Commun.

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Molecular imprinting is an approach of generating imprinting cavities in polymer structures that are compatible with the target molecules. The cavities have memory for shape and chemical recognition, similar to the recognition mechanism of antigen–antibody in organisms. Their structures are also called biomimetic receptors or synthetic receptors. Owing to the excellent selectivity and unique structural predictability of molecularly imprinted materials (MIMs), practical MIMs have become a rapidly evolving research area providing key factors for understanding separation, recognition, and regenerative properties toward biological small molecules to biomacromolecules, even cell and microorganism. In this review, the characteristics, morphologies, and applicability of currently popular carrier materials for molecular imprinting, especially the fundamental role of hydrogels, porous materials, hierarchical nanoparticles, and 2D materials in the separation and recognition of biological templates are discussed. Moreover, through a series of case studies, emphasis is given on introducing imprinting strategies for biological templates with different molecular scales. In particular, the differences and connections between small molecular imprinting (bulk imprinting, “dummy” template imprinting, etc.), large molecular imprinting (surface imprinting, interfacial imprinting, etc.), and cell imprinting strategies are demonstrated in detail. Finally, future research directions are provided.

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“…These soft, smart, and stimuli‐responsive materials are chemosensitive, electroactive under low driving voltage, and biocompatible . They are extensively used in the development of artificial implants such as artificial muscles or soft actuators and scaffolds that mimic the native extracellular matrices …”

Section: Introductionmentioning

confidence: 99%

“…Extensive work has been reported on the sensing properties and actuation mechanism of PAAM hydrogels as on their application as scaffolds for tissue engineering . However, few of them are concerned about the effect of the thermodynamic parameters like temperature and pressure, applied during the free radical polymerization, on the properties of the resulting hydrogel .…”

Section: Introductionmentioning

confidence: 99%

Templating polyacrylamide hydrogel for interconnected microstructure and improved performance

Bassil

Moussa

Tahchi

2018

J of Applied Polymer Sci

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In this study, the pressure and temperature are monitored during acrylamide polymerization and their effects on the mechanical properties and swelling of the resulting hydrogel are investigated. The polymerization kinetic and network formation mechanism are correlated to the environmental thermodynamic conditions under which the hydrogels are polymerized. Then, the swelling and Young's modulus are measured and shown to be tunable along a wide range of values. The swelling ratio varies between 50 and 2262 while Young's modulus varies between 10.99 and 40.70 kPa. In addition, the formation of macroporous hydrogel with channel like structures along the vacuum direction under a reduced pressure of 5 mbar is reported. The macroporous hydrogel has a modulus of 40.70 kPa and shrink approximatively three times faster than the hydrogel polymerized under normal pressure and has a modulus of 10.99 kPa. Hence, this interconnected network can overcome the fluid diffusion limitations of bulk hydrogels without compromising the mechanical properties.

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Programmed cell adhesion and growth on cell-imprinted polyacrylamide hydrogels

Cited by 35 publications

References 43 publications

Vision for Functionally Decorated and Molecularly Imprinted Polymers in Regenerative Engineering

Vision for Functionally Decorated and Molecularly Imprinted Polymers in Regenerative Engineering

Molecularly Imprinted Materials for Selective Biological Recognition

Templating polyacrylamide hydrogel for interconnected microstructure and improved performance

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