Programmed death-1 mediates venous neointimal hyperplasia in humans and rats

Sun, Peng; Wang, Zhiwei; Liu, Weizhen; Li, Mingxing; Wei, Shunbo; Xu, Yu; Qiao, Zhentao; Wang, Wang; Fu, Yang; Bai, Hualong; Li, Jingan

doi:10.18632/aging.203185

Cited by 9 publications

(6 citation statements)

References 30 publications

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“…Rats were sacrificed on postoperative days 14 and the patches were explanted for analysis; since we found that neointimal hyperplasia was consistent after day 7 in our previous studies 23 , 29 , 38 . we chose day 14 as the observational time point 25 , 26 27 . No immunosuppressive agents, antibiotics, antiplatelet agents, or heparin were given at any time.…”

Section: Methodsmentioning

confidence: 99%

“…Previously we showed that pericardial patches can be coated with rapamycin-containing nanoparticles to inhibit neointimal hyperplasia 23 . The decellularized pericardial patch 23 , decellularized human saphenous vein patch 25 , and rat thoracic aorta patch 26 , 27 can also be coated with heparin or PD-1 antibody to decrease neointimal thickness. We hypothesized that decellularized fish swim bladder can serve as a biological vascular graft scaffold and can be surface modified; accordingly, we created swim bladder-derived scaffolds, coated them with rapamycin, shaped them into patches or tubes, and implanted them into rats to determine biocompatibility.…”

Section: Introductionmentioning

confidence: 99%

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The application of tissue-engineered fish swim bladder vascular graft

Bai

Sun

et al. 2021

Commun Biol

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Small diameter (< 6 mm) prosthetic vascular grafts continue to show very low long-term patency, but bioengineered vascular grafts show promising results in preclinical experiments. To assess a new scaffold source, we tested the use of decellularized fish swim bladder as a vascular patch and tube in rats. Fresh goldfish (Carassius auratus) swim bladder was decellularized, coated with rapamycin and then formed into patches or tubes for implantation in vivo. The rapamycin-coated patches showed decreased neointimal thickness in both the aorta and inferior vena cava patch angioplasty models. Rapamycin-coated decellularized swim bladder tubes implanted into the aorta showed decreased neointimal thickness compared to uncoated tubes, as well as fewer macrophages. These data show that the fish swim bladder can be used as a scaffold source for tissue-engineering vascular patches or vessels.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The application of tissue-engineered fish swim bladder vascular graft

Bai

Sun

et al. 2021

Commun Biol

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“…Neointimal cell, especially smooth muscle cells proliferation also plays an important role in the neointimal hyperplasia, so rapamycin or paclitaxel coated grafts are also widely used to inhibit neointimal cell proliferation (Bai et al., 2021 ). Recent studies showed programmed death-1 antibody or inhibitor coated vascular graft can also inhibit neointimal hyperplasia after patch angioplasty in rat (Sun et al., 2021 , Bai et al., 2021 ). IL-33 antibody absorbed plant leaf patch can be used to inhibit venous neointimal hyperplasia in rat (Xie et al., 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Delivery of rivaroxaban and chitosan rapamycin microparticle with dual antithrombosis and antiproliferation functions inhibits venous neointimal hyperplasia

Sun

et al. 2022

Drug Delivery

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Neointimal hyperplasia is a complex process after vascular interventions, acute platelet deposition and smooth muscle cell proliferation both contributed to this process. There are still no perfect solutions to solve this problem. Rivaroxaban is a novel anticoagulant that has been widely used in clinic, it has a good pharmacological effects both in vivo and in vitro. Chitosan microparticle rapamycin (MP-rapa) was fabricated, interspaces of polyglycolic acid (PGA) scaffold were used as a reservoir of MP-rapa, and the scaffold was coated with hyaluronic acid rivaroxaban (MP-rapa-riva). Scanning electronic microscopy (SEM) photographs were taken and water contact angles were measured, rat inferior vena cava (IVC) patch venoplasty model was used; patches were harvested at day 14 and examined by immunohistochemistry and immunofluorescence. SEM photographs showed the microparticles rapamycin were inside the interspace of the scaffold, hyaluronic acid rivaroxaban was also successfully coated onto the surface of the scaffold. There was a thinner neointima, fewer proliferating cell nuclear antigen (PCNA) positive cells, fewer macrophages in the MP-rapa and MP-rapa-riva grafts compared to the control PGA graft. The result showed that this scaffold with dual anticoagulation and antiproliferation functions can effectively inhibit venous neointimal hyperplasia, although this is an animal experiment, it showed promising potential clinical application in the future.

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“…Fourier infrared spectroscopy (FTIR, Thermo Nicolet 6700, Thermo Fisher Scientific, Waltham, MA, USA) was used to analyze the functional groups on the sample surface [ 43 ]. Water contact angle measuring instrument (JC2000C, Powereach, Shanghai, China) was used to detect the water contact angle (WCA) of each sample to assess their hydrophilicity [ 44 ]. In order to evaluate the distribution of ECM on the samples, two major proteins in the ECM, type IV collagen (COL IV), and fibronectin (FN) [ 45 , 46 ] were selected to be characterized quantitatively and qualitatively.…”

Section: Methodsmentioning

confidence: 99%

Tailoring ZE21B Alloy with Nature-Inspired Extracellular Matrix Secreted by Micro-Patterned Smooth Muscle Cells and Endothelial Cells to Promote Surface Biocompatibility

Liu

Chen

Zhang

et al. 2022

IJMS

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Delayed surface endothelialization is a bottleneck that restricts the further application of cardiovascular stents. It has been reported that the nature-inspired extracellular matrix (ECM) secreted by the hyaluronic acid (HA) micro-patterned smooth muscle cells (SMC) and endothelial cells (EC) can significantly promote surface endothelialization. However, this ECM coating obtained by decellularized method (dECM) is difficult to obtain directly on the surface of degradable magnesium (Mg) alloy. In this study, the method of obtaining bionic dECM by micro-patterning SMC/EC was further improved, and the nature-inspired ECM was prepared onto the Mg-Zn-Y-Nd (ZE21B) alloy surface by self-assembly. The results showed that the ECM coating not only improved surface endothelialization of ZE21B alloy, but also presented better blood compatibility, anti-hyperplasia, and anti-inflammation functions. The innovation and significance of the study is to overcome the disadvantage of traditional dECM coating and further expand the application of dECM coating to the surface of degradable materials and materials with different shapes.

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Programmed death-1 mediates venous neointimal hyperplasia in humans and rats

Cited by 9 publications

References 30 publications

The application of tissue-engineered fish swim bladder vascular graft

The application of tissue-engineered fish swim bladder vascular graft

Delivery of rivaroxaban and chitosan rapamycin microparticle with dual antithrombosis and antiproliferation functions inhibits venous neointimal hyperplasia

Tailoring ZE21B Alloy with Nature-Inspired Extracellular Matrix Secreted by Micro-Patterned Smooth Muscle Cells and Endothelial Cells to Promote Surface Biocompatibility

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