Programmed Nanopatterning of Organic/Inorganic Nanoparticles Using Nanometer‐Scale Wells Embedded in a DNA Origami Scaffold

Abstract

Gold nanoparticles are selectively patterned into nanoarrays by using a punched DNA origami scaffold with nanometer‐sized periodical wells. By simply adding unmodified gold nanoparticle into the solution of the punched origami scaffold, exactly one particle is captured selectively in a thiolated well. The capture is completely chemoselective such that an alternating streptavidin/gold nanoparticle heteroarray with a 26 nm period is successfully prepared.

DNA nanostructures as scaffolds for metal nanoparticles

“…We recently proposed a new strategy to assemble nanoarrays of individual NPs on DNA origami scaffolds. [34][35][36][37][38][39] This strategy is based on our previous finding that a nm-sized cavity (a DNA well) embedded in 2-nm-thick DNA sheets, including DNA origami, can serve as a well to precisely capture one protein molecule inside and stably accommodate it under repetitive AFM scanning. 34,35 This approach is applicable to other NPs of similar diameters, such as AuNPs.…”

“…34,35 This approach is applicable to other NPs of similar diameters, such as AuNPs. 36 We designed a stick-like punched DNA origami structure with nine wells with dimensions of 7 Â 14 Â 2 nm (Figure 4). Two of the edges of each well were modified with a dithiol residue (dithiol phosphoramidite (DTPA)), which is known to form significantly stronger bonds with AuNPs than simple monothiols, similar to the lipoic acids used by Yan and Liu, via a 2.3-nm long triethylene glycol linker at the end of appropriate staple strands (the anchor strands; Figure 5a).…”

Chemistry Can Make Strict and Fuzzy Controls for Bio-Systems: DNA Nanoarchitectonics and Cell-Macromolecular Nanoarchitectonics

“…Nanoarrays of gold nanoparticles were also prepared by attaching oligonucleotide strands to the nanoparticles with a dithiol residue. 261 Exactly one gold nanoparticle was selectively captured in a predetermined well in punched DNA origami.…”

Engineered RNA Nanodesigns for Applications in RNA Nanotechnology

“…The use of these rules has resulted in the engineering and characterization of numerous DNA 3D nanoscaffolds with different connectiviities [7][8][9][10][11][12][13][14][15][16][17][18] and the ability for some of them to promote targeted delivery by functioning as DNA nano-capsules [19][20][21] or DNA nano-carriers for other functionialities [22]. Another powerful technique called DNA "origami", developed by Paul Rothemund [23], has been extended from its original scope for designing different 2D DNA shapes [24] and functional templates [25][26][27][28] to the creation of 3D objects such as a pyramidal tetrahedron [29], a functional nano-box [30,31] and a nanorobot [32], or shapes [33] relying on tensegrity [34] (structural integrity maintained by opposed tension in internal components) or helix bundles [35]. Similarly, Chad Mirkin and colleagues have done significant work using DNA oligonucleotides to form nanoparticle (NP) probes [36] by modifying colloidal nanoparticles with oligonucleotides which, upon the introduction of a complementary sequence, allow the self-assembly of nanoparticles into two-and three-dimensional architectures [37,38].…”

“…The use of these rules has resulted in the engineering and characterization of numerous DNA 3D nanoscaffolds with different connectiviities [7][8][9][10][11][12][13][14][15][16][17][18] and the ability for some of them to promote targeted delivery by functioning as DNA nano-capsules [19][20][21] or DNA nano-carriers for other functionialities [22]. Another powerful technique called DNA "origami", developed by Paul Rothemund [23], has been extended from its original scope for designing different 2D DNA shapes [24] and functional templates [25][26][27][28] Abstract Nucleic acids have emerged as an extremely promising platform for nanotechnological applications because of their unique biochemical properties and functions. RNA, in particular, is characterized by relatively high thermal stability, diverse structural flexibility, and its capacity to perform a variety of functions in nature.…”