Progress in densitometry for quantitation in planar chromatography

Poole, Colin F.; Poole, Salwa K.

doi:10.1016/s0378-4347(00)84479-5

Cited by 56 publications

(17 citation statements)

References 154 publications

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“…Thin layer chromatography made a great progress and attained a wide acceptance as a major analytical tool for both qualitative and quantitative methods of analysis and became a well established method for the assay of drugs in mixture (Poole and Poole, 1989). It has many applications in the field of pharmaceutical studies which include: stability, impurities, synthetic drugs, pharmacokinetic, enantiomeric purity and drug monitoring in biological fluids (Mincsovics et al, 2001) (Aboul Enein et al, 2003.…”

Section: Discussionmentioning

confidence: 99%

Stability indicating HPTLC method for determination of Metopimazine in pharmaceutical formulation and human plasma

Naguib

Abdelrahman

2014

Beni-Suef University Journal of Basic and Applied Sciences

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Oxidative degradationAlkaline degradation HPTLC Pharmaceutical formulation a b s t r a c t Accurate, selective and sensitive stability indicating high performance thin layer chromatographic (HPTLC) method was developed and validated for determination of Metopimazine (MPZ) in presence of its degradation products; including the oxidative degradation product (Deg I) and the alkaline hydrolysis degradation product (Deg II; formerly reported as acid metabolite). The proposed method was applied for analysis of MPZ in pure form, pharmaceutical formulation (tablets) and human plasma over the concentration range of 0.4e1.4 mg band À1 with accuracy of mean percentage recovery 100.10 AE 0.941. The method depends on separation of MPZ from its degradation products on HPTLC silica gel 60 F 254 plates using chloroformemethanoleammonia (12:2:0.1, by volume) as developing system followed by densitometric measurement of bands at 254 nm. The method was validated according to ICH guidelines. The degradation products were well resolved from the pure drug with significantly different R f values (MPZ, R f ¼ 0.58, Deg I, R f ¼ 0.35 and Deg II, R f ¼ 0.03). The results obtained by the proposed method were statistically compared to the reported HPTLC method, and there was no significant difference between them regarding both accuracy and precision; indicating the ability of the proposed method to be used for routine analysis of drug product.

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Section: Discussionmentioning

confidence: 99%

Stability indicating HPTLC method for determination of Metopimazine in pharmaceutical formulation and human plasma

Naguib

Abdelrahman

2014

Beni-Suef University Journal of Basic and Applied Sciences

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“…Commercial systems for in situ quantification can be categorized as monochromatic slit scanning densitometers, diode-array scanners, and image analyzers (charged-coupled device cameras and flatbed scanners) [9,12,[53][54][55]. Specific detectors for radioisotopes [56] and interfaces for direct evaluation by spectrosocopic instruments (mass spectrometry and infrared and Raman spectroscopy) [57][58][59][60][61] are also available.…”

Section: Densitometrymentioning

confidence: 99%

“…17, share several features in common [9,[53][54][55]64]. They usually allow measurements in the reflectance mode by absorbance or fluorescence.…”

Section: Slit-scanning Densitometersmentioning

confidence: 99%

“…Image analyzers in the form of a digital camera (chargedcoupled device or web cam) or office peripherals (flatbed scanner) with appropriate software have grown in popularity in recent years [9,12,[53][54][55]71,72]. These are generally simpler and less expensive than scanning densitomers but lack some of the advanced features provided by the latter.…”

Section: Image Analyzersmentioning

confidence: 99%

“…Laboratory-built systems for electronic scanning using digital cameras of varying sophistication have been described [53,77,78]. These systems consist of some combination of a computer with video digitizer, light source, monochromator, and appropriate optics to illuminate the plate and focus the image onto a chargedcoupled device (CCD).…”

Section: Charged-coupled Devicesmentioning

confidence: 99%

See 2 more Smart Citations

Instrument platforms for thin-layer chromatography

Bernard-Savary¹,

Poole

2015

Journal of Chromatography A

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Planar Chromatography in Clinical Chemistry

Jain

Sherma

2000

Encyclopedia of Analytical Chemistry

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Planar chromatography (PC) like any other chromatographic technique is a multistage distribution process. It is a form of liquid chromatography in which the stationary phase is supported on a planar surface rather than a column. Separations in PC occur because of differential migration velocities through the sorbent layer in a fixed separation time. The sample zones are fixed in space at the completion of the separation, allowing off‐line monitoring. There are two planar techniques: paper chromatography and thin‐layer chromatography (TLC). In recent years, TLC has superseded paper chromatography owing to greater speed, versatility, and reproducibility. Improvements in techniques and equipment as well as the ready availability of high‐quality plates with uniform layers have extended and increased the use of TLC in clinical chemistry and other disciplines, offering quantitative as well as qualitative analysis. High‐performance thin‐layer chromatography (HPTLC), in particular, has developed to the extent that separation and quantitation can provide results that are comparable with other analytical methods such as high‐pressure liquid chromatography (HPLC). The widespread application of TLC is also due to the fact that it is simple, robust, and flexible, and has a large sample throughput. Furthermore, coupled spectroscopic methods (TLC/mass spectrometry (TLC/MS), TLC/Fourier transform infrared spectrometry (TLC/FTIR) etc.) have considerably enhanced the reproducibility of TLC. TLC is often critically compared with column chromatography such as HPLC. This is a matter of endless dispute. Each technique has its own advantages and limitations. TLC is only inexpensive when qualitative results are needed. When reliable quantitative data are required, TLC is as expensive as column chromatography. In the authors' opinion, complementary rather than competitive chromatography offers the best solution to analytical requirements.

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Progress in densitometry for quantitation in planar chromatography

Cited by 56 publications

References 154 publications

Stability indicating HPTLC method for determination of Metopimazine in pharmaceutical formulation and human plasma

Stability indicating HPTLC method for determination of Metopimazine in pharmaceutical formulation and human plasma

Instrument platforms for thin-layer chromatography

Planar Chromatography in Clinical Chemistry

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