Progress of CRISPR/Cas9 technology in breeding of Brassica napus

Chang, Tao; Guan, Mei; Zhou, Bingqian; Peng, Zechuan; Xing, Min; Wang, Xiaodan; Guan, Chunyun

doi:10.1016/j.ocsci.2021.03.005

Cited by 13 publications

(6 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The research teams at the Chinese Academy of Engineering and Hunan Agricultural University, have formulated the national standard for high-oleic rapeseed (NY/T 3786–2020), and its production has officially become an industrialized system. At present, the strategic goal for oilseed rape production is to further improve the quality and healthcare properties while ensuring or maintaining a high yield [ 5 , 6 ]. However, the production of high-oleic rape has been affected by problems such as reduced yield, prolonged growth period, low economic benefit, and limited promotion, which are affected by the breeding process [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive evaluation of high-oleic rapeseed (Brassica napus) based on quality, resistance, and yield traits: A new method for rapid identification of high-oleic acid rapeseed germplasm

Chang

et al. 2022

PLoS ONE

Self Cite

View full text Add to dashboard Cite

To scientifically evaluate and utilize high-oleic acid rape germplasm resources and cultivate new varieties suitable for planting in the Hunan Province, 30 local high-oleic acid rape germplasms from Hunan were used as materials. The 12 personality indices of quality, yield, and resistance were comprehensively evaluated by variability, correlation, principal component, and cluster analyses. The results of variability showed that except for oleic acid, the lowest coefficient of variation was oil content, which was 0.06. Correlation analysis showed that oil content was positively correlated with main traits such as yield per plant and oleic acid, which could be used in the early screening of high-oleic rape germplasm. The results of principal component analysis showed that the 12 personality indicators were integrated into four principal components, and the cumulative contribution rate was 62.487%. The value of comprehensive coefficient ‘F’ was positively correlated with the first, second, and fourth principal components and negatively correlated with the third principal component. Cluster analysis showed that 30 high-oleic rape germplasms could be divided into four categories consisting of 9 (30%), 6 (20%), 7 (23%), and 8 (27%) high-oleic rape germplasms, each with the characteristics of "high disease resistance", "high yield", "high protein", and "more stability". This study not only provides a reference basis for high-oleic rape breeding but also provides a theoretical basis for their early screening.

show abstract

Section: Introductionmentioning

confidence: 99%

Comprehensive evaluation of high-oleic rapeseed (Brassica napus) based on quality, resistance, and yield traits: A new method for rapid identification of high-oleic acid rapeseed germplasm

Chang

et al. 2022

PLoS ONE

Self Cite

View full text Add to dashboard Cite

show abstract

“…The advent of clustered regularly interspaced short palindromic repeats systems associated with Cas enzymes (CRISPR/Cas) has opened the doors for widespread editing in oilseed Brassicas , which greatly benefit from sharing homologs of well characterised genes associated with yield in Arabidopsis . However, the high gene homology and copy number of the allotetraploid Brassicas , such as B. napus and B. juncea , present a challenge in terms of editing accuracy, in particular single base editing [ 118 ], and in gene redundancy via multiple homologs, requiring all genes homologous to the target to be modified to produce a reliable phenotype [ 119 ]. Polygenic traits will require a more intricate multiplex approach, targeting more than one locus in a single round of editing.…”

Section: Developing New Phenotypes Via Genome Editing Technologymentioning

confidence: 99%

“…Hence, many of the traits currently targeted for yield improvement are those that are closely related to yield, or those that are indirectly related, but highly correlated. For example, current research has mainly focused on increasing seed number through changes to silique structure and improving harvestability through a reduction in plant heigh and an increase in plant density [ 118 ]. In addition, other traits such as pod shattering resistance [ 123 , 124 ] and flowering time [ 125 ], which significantly influence yield, have been investigated.…”

Section: Developing New Phenotypes Via Genome Editing Technologymentioning

confidence: 99%

The Global Assessment of Oilseed Brassica Crop Species Yield, Yield Stability and the Underlying Genetics

Zandberg

Fernandez

Danilevicz

et al. 2022

Plants

View full text Add to dashboard Cite

The global demand for oilseeds is increasing along with the human population. The family of Brassicaceae crops are no exception, typically harvested as a valuable source of oil, rich in beneficial molecules important for human health. The global capacity for improving Brassica yield has steadily risen over the last 50 years, with the major crop Brassica napus (rapeseed, canola) production increasing to ~72 Gt in 2020. In contrast, the production of Brassica mustard crops has fluctuated, rarely improving in farming efficiency. The drastic increase in global yield of B. napus is largely due to the demand for a stable source of cooking oil. Furthermore, with the adoption of highly efficient farming techniques, yield enhancement programs, breeding programs, the integration of high-throughput phenotyping technology and establishing the underlying genetics, B. napus yields have increased by >450 fold since 1978. Yield stability has been improved with new management strategies targeting diseases and pests, as well as by understanding the complex interaction of environment, phenotype and genotype. This review assesses the global yield and yield stability of agriculturally important oilseed Brassica species and discusses how contemporary farming and genetic techniques have driven improvements.

show abstract

“…The first gene-editing in a horticultural crop was reported in B. oleracea which was achieved via a TALEN ( Sun et al, 2013 ). After this first report, in the following years, significant progress has been made in Brassica crops, especially in B. napus ( Chang et al, 2021 ) targeting several genes such as yield related genes ( Braatz et al, 2017 ; Yang et al, 2017 ; Zhai et al, 2019 ), quality related genes ( Okuzaki et al, 2018 ; Zhang et al, 2019 ), genes related to plant architecture ( Zheng M. et al, 2020 ), seed coat color related genes ( Zhai et al, 2020 ) and disease resistance genes ( Sun et al, 2018 ). But the function of the genes targeted by gene-editing in Brassica crops have mostly focused on the genes affecting development, metabolism and not much attention has been paid to target biotic stress-response genes except a few.…”

Section: Downy Mildewmentioning

confidence: 99%

Molecular Breeding Strategy and Challenges Towards Improvement of Downy Mildew Resistance in Cauliflower (Brassica oleracea var. botrytis L.)

et al. 2021

View full text Add to dashboard Cite

Cauliflower (Brassica oleracea var. botrytis L.) is one of the important, nutritious and healthy vegetable crops grown and consumed worldwide. But its production is constrained by several destructive fungal diseases and most importantly, downy mildew leading to severe yield and quality losses. For sustainable cauliflower production, developing resistant varieties/hybrids with durable resistance against broad-spectrum of pathogens is the best strategy for a long term and reliable solution. Identification of novel resistant resources, knowledge of the genetics of resistance, mapping and cloning of resistance QTLs and identification of candidate genes would facilitate molecular breeding for disease resistance in cauliflower. Advent of next-generation sequencing technologies (NGS) and publishing of draft genome sequence of cauliflower has opened the flood gate for new possibilities to develop enormous amount of genomic resources leading to mapping and cloning of resistance QTLs. In cauliflower, several molecular breeding approaches such as QTL mapping, marker-assisted backcrossing, gene pyramiding have been carried out to develop new resistant cultivars. Marker-assisted selection (MAS) would be beneficial in improving the precision in the selection of improved cultivars against multiple pathogens. This comprehensive review emphasizes the fascinating recent advances made in the application of molecular breeding approach for resistance against an important pathogen; Downy Mildew (Hyaloperonospora parasitica) affecting cauliflower and Brassica oleracea crops and highlights the QTLs identified imparting resistance against this pathogen. We have also emphasized the critical research areas as future perspectives to bridge the gap between availability of genomic resources and its utility in identifying resistance genes/QTLs to breed downy mildew resistant cultivars. Additionally, we have also discussed the challenges and the way forward to realize the full potential of molecular breeding for downy mildew resistance by integrating marker technology with conventional breeding in the post-genomics era. All this information will undoubtedly provide new insights to the researchers in formulating future breeding strategies in cauliflower to develop durable resistant cultivars against the major pathogens in general and downy mildew in particular.

show abstract

Progress of CRISPR/Cas9 technology in breeding of Brassica napus

Cited by 13 publications

References 27 publications

Comprehensive evaluation of high-oleic rapeseed (Brassica napus) based on quality, resistance, and yield traits: A new method for rapid identification of high-oleic acid rapeseed germplasm

Comprehensive evaluation of high-oleic rapeseed (Brassica napus) based on quality, resistance, and yield traits: A new method for rapid identification of high-oleic acid rapeseed germplasm

The Global Assessment of Oilseed Brassica Crop Species Yield, Yield Stability and the Underlying Genetics

Molecular Breeding Strategy and Challenges Towards Improvement of Downy Mildew Resistance in Cauliflower (Brassica oleracea var. botrytis L.)

Contact Info

Product

Resources

About