Progress of metal nanoclusters in nucleic acid detection

Lin, Xia; Zou, Lianjia; Lan, Weisen; Liang, Chunxian; Yin, Yanchun; Liang, Jian; Zhou, Yuanming; Wang, Jianyi

doi:10.1039/d1dt03183j

Cited by 11 publications

(8 citation statements)

References 69 publications

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“…30 They have also been successfully used to detect a wide range of analytes, including biomarkers, drugs, biomolecules, and nucleic acids. [31][32][33][34] Nowadays, MoNCs which are not been explored much yet have become a fascinating topic in nanoscience and analytical chemistry research. 35,36 For example, 6-aza-2-thiothymine (ATT) capped MoNCs have been fabricated to recognize cancer drug methotrexate with a LOD of 1.28 nM.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of molybdenum nanoclusters from Vitex negundo leaves for sensing epinephrine in a pharmaceutical composition

et al. 2023

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Section: Introductionmentioning

confidence: 99%

Synthesis of molybdenum nanoclusters from Vitex negundo leaves for sensing epinephrine in a pharmaceutical composition

et al. 2023

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“…Such polymeric ligands preserve the distinctive electronic structure of silver clusters, resulting in aqueous stable chromophores. Biopolymers also encapsulate silver molecules, as illustrated by single-stranded oligonucleotides that protect their cluster adducts in harsh aqueous and biological environments. − DNA–silver conjugates are recognized by their strong emission and diverse spectra, and one notable application of these chromophores is in biological imaging. − Here, conjugates with far-red and near-infrared luminescence can be identified more confidently because the endogenous background is lower relative to the visible region. − The cluster spectra span the violet to near-infrared range and can be controlled by the DNA host.…”

Section: Introductionmentioning

confidence: 99%

Breaching the Fortress: Photochemistry of DNA-Caged Ag₁₀⁶⁺

Setzler,

Arrington,

Lewis

et al. 2023

J. Phys. Chem. B

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A DNA strand can encapsulate a silver molecule to create a nanoscale, aqueous stable chromophore. A protected cluster that strongly fluoresces can also be weakly photolabile, and we describe the laser-driven photochemistry of the green fluorophore C 4 AC 4 TC 3 GT 4 /Ag 10 6+ . The embedded cluster is selectively photoexcited at 490 nm and then bleached, and we describe how the efficiency, products, and route of this photochemical reaction are controlled by the DNA cage. With irradiation at 496.5 nm, the cluster absorption progressively drops to give a photodestruction quantum yield of 1.5 (±0.2) × 10 –4 , ∼10 3 × less efficient than fluorescence. A new λ abs = 335 nm chromophore develops because the precursor with 4 Ag 0 is converted into a group of clusters with 2 Ag 0 – Ag 6 4+ , Ag 7 5+ , Ag 8 6+ , and Ag 9 7+ . The 4–7 Ag + in this series are chemically distinct from the 2 Ag 0 because they are selectively etched by iodide. This halide precipitates silver to favor only the smallest Ag 6 4+ cluster, but the larger clusters re-develop when the precipitated Ag + ions are replenished. DNA-bound Ag 10 6+ decomposes because it is electronically excited and then reacts with oxygen. This two-step process may be state-specific because O 2 quenches the red luminescence from Ag 10 6+ . However, the rate constant of 2.3 (±0.2) × 10 6 M –1 s –1 is relatively small, which suggests that the surrounding DNA matrix hinders O 2 diffusion. On the basis of analogous photoproducts with methylene blue, we propose that a reactive oxygen species is produced and then oxidizes Ag 10 6+ to leave behind a loose Ag + -DNA skeleton. These findings underscore the ability of DNA scaffolds to not only tune the spectra but also guide the reactions of their molecular silver adducts.

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“…Nucleobases preferentially coordinate silvers but with different affinities, so a particular pattern of nucleobase ligands encodes the cluster spectra. ,,,− Second, DNA structure shapes binding sites. A single-stranded oligonucleotide can both encapsulate a cluster and hybridize with a complement. ,− Complementary base pairs block cluster binding sites on nucleobases and force a DNA-bound cluster to reorganize, which can enhance emission by ≲10 3 fold. Third, a flexible DNA wraps around a cluster to give compact and emissive conjugates because hinge sites allow the oligonucleotide to fold. − Fourth, DNA strands assemble around a shared cluster.…”

Section: Introductionmentioning

confidence: 99%

Tug-of-War between DNA Chelation and Silver Agglomeration in DNA–Silver Cluster Chromophores

et al. 2022

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Supramolecular chromophores form when a DNA traps silvers that then coalesce into clusters with discrete, molecular electronic states. However, DNA strands are polymeric ligands that disperse silvers and thus curb agglomeration. We study this competition using two chromophores that share three common components: a dimeric DNA scaffold, Ag + -nucleobase base pairs, and Ag 0 chromophores. The DNA host C 4 -A 2 -iC 4 T mimics structural elements in a DNA-cluster crystal structure using a phosphodiester backbone with combined 5′ → 3′ and 3′ → 5′ (indicated by "i") directions. The backbone directions must alternate to form the two silver clusters, and this interdependence supports a silver-linked structure. This template creates two chromophores with distinct sizes, charges, and hence spectra: (C 4 -A 2 -iC 4 T) 2 /Ag 11 7+ with λ abs /λ em = 430/520 nm and (C 4 -A 2 -iC 4 T) 2 /Ag 14 8+ with λ abs /λ em = 510/630 nm. The Ag + and Ag 0 constituents in these partially oxidized clusters are linked with structural elements in C 4 -A 2 -iC 4 T. Ag + alone binds sparsely but strongly to form C 4 -A 2 -iC 4 T/3−4 Ag + and (C 4 -A 2 -iC 4 T) 2 /7−8 Ag + complexes, and these stoichiometries suggest that Ag + cross-links pairs of cytosines to form a hairpin with a metallo-C 4 /iC 4 duplex and an adenine loop. The Ag 0 are chemically orthogonal because they can be oxidatively etched without disrupting the underlying Ag + −DNA matrix, and their reactivity is attributed to their valence electrons and weaker chelation by the adenines. These studies suggest that Ag + disperses with the cytosines to create an adenine binding pocket for the Ag 0 cluster chromophores.

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Progress of metal nanoclusters in nucleic acid detection

Abstract: Metal nanoclusters (MNCs), including Ag NCs, Cu NCs, and Au NCs, can be used as fluorescent probes for nucleic acid testing. Identification of DNA fragments and trace detection of miRNA (LOD as low as aM) can be achieved.

Cited by 11 publications

References 69 publications

Synthesis of molybdenum nanoclusters from Vitex negundo leaves for sensing epinephrine in a pharmaceutical composition

Synthesis of molybdenum nanoclusters from Vitex negundo leaves for sensing epinephrine in a pharmaceutical composition

Breaching the Fortress: Photochemistry of DNA-Caged Ag₁₀⁶⁺

Tug-of-War between DNA Chelation and Silver Agglomeration in DNA–Silver Cluster Chromophores

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Progress of metal nanoclusters in nucleic acid detection

Abstract: Metal nanoclusters (MNCs), including Ag NCs, Cu NCs, and Au NCs, can be used as fluorescent probes for nucleic acid testing. Identification of DNA fragments and trace detection of miRNA (LOD as low as aM) can be achieved.

Cited by 11 publications

References 69 publications

Synthesis of molybdenum nanoclusters from Vitex negundo leaves for sensing epinephrine in a pharmaceutical composition

Synthesis of molybdenum nanoclusters from Vitex negundo leaves for sensing epinephrine in a pharmaceutical composition

Breaching the Fortress: Photochemistry of DNA-Caged Ag106+

Tug-of-War between DNA Chelation and Silver Agglomeration in DNA–Silver Cluster Chromophores

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Product

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Breaching the Fortress: Photochemistry of DNA-Caged Ag₁₀⁶⁺