Progress on characterization of self-incompatibility in Brassica napus L.

Zhang, Xingguo; Yin, Dongmei; Zhu, Wei; Ma, Chaozhi; Fu, Tingdong

doi:10.1007/s10681-011-0474-2

Cited by 4 publications

(5 citation statements)

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“…This will allow the approach of commercial yellow mustard breeding programs to be switched from improving outcrossing populations to developing more uniform elite inbred line varieties. In addition, the availability of dominant and recessive SC gene sources in B. napus would allow the self-incompatibility reproduction system to be used to produce three-way hybrids (Fu 1981 ; Zhang et al 2011 ). In such a system, the recessive SC gene source is used as maintainer (B) for the SI line while the dominant SC gene is used as restorer (R) line to produce SC F 1 hybrids.…”

Section: Discussionmentioning

confidence: 99%

Self-(in)compatibility inheritance and allele-specific marker development in yellow mustard (Sinapis alba)

Zeng

Cheng

2013

Mol Breeding

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Yellow mustard (Sinapis alba) has a sporophytic self-incompatibility reproduction system. Genetically stable self-incompatible (SI) and self-compatible (SC) inbred lines have recently been developed in this crop. Understanding the S haplotype of different inbred lines and the inheritance of the self-(in)compatibility (SI/SC) trait is very important for breeding purposes. In this study, we used the S-locus gene-specific primers in Brassica rapa and Brassica oleracea to clone yellow mustard S-locus genes of SI lines Y514 and Y1130 and SC lines Y1499 and Y1501. The PCR amplification results and DNA sequences of the S-locus genes revealed that Y514 carried the class I S haplotype, while Y1130, Y1499, and Y1501 had the class II S haplotype. The results of our genetic studies indicated that self-incompatibility was dominant over self-compatibility and controlled by a one-gene locus in the two crosses of Y514 × Y1499 and Y1130 × Y1501. Of the five S-locus gene polymorphic primer pairs, Sal-SLGI and Sal-SRKI each generated one dominant marker for the SI phenotype of Y514; Sal-SLGII and Sal-SRKII produced dominant marker(s) for the SC phenotype of Y1501 and Y1499; Sal-SP11II generated one dominant marker for Y1130. These markers co-segregated with the SI/SC phenotype in the F2 populations of the two crosses. In addition, co-dominant markers were developed by mixing the two polymorphic primer pairs specific for each parent in the multiplex PCR, which allowed zygosity to be determined in the F2 populations. The SI/SC allele-specific markers have proven to be very useful for the selection of the desirable SC genotypes in our yellow mustard breeding program.Electronic supplementary materialThe online version of this article (doi:10.1007/s11032-013-9943-8) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Self-(in)compatibility inheritance and allele-specific marker development in yellow mustard (Sinapis alba)

Zeng

Cheng

2013

Mol Breeding

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“…The SI of 'S-1300' is recessive to BnS-1/BnS-6 lines but dominant to BnS-7/BnS-6 lines [23,24,29]. A perfect tribe-cross hybrid seed produce system was established by combining 'S-1300' with BnS-1/BnS-6 lines (SI restorer lines) and BnS-7/BnS-6 lines (SI maintainer lines) [24,30]. However, the selfincompatible accessions in floral morphologies are similar to the self-compatible accessions, meaning that it is difficult to discriminate the contaminated plants in SI breeding lines [23].…”

Section: Introductionmentioning

confidence: 99%

Characterization of a Common S Haplotype BnS-6 in the Self-Incompatibility of Brassica napus

Liu

Yong

et al. 2021

Plants

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Self-incompatibility (SI) is a pollen-stigma recognition system controlled by a single and highly polymorphic genetic locus known as the S-locus. The S-locus exists in all Brassica napus (B. napus, AACC), but natural B. napus accessions are self-compatible. About 100 and 50 S haplotypes exist in Brassica rapa (AA) and Brassica oleracea (CC), respectively. However, S haplotypes have not been detected in B. napus populations. In this study, we detected the S haplotype distribution in B. napus and ascertained the function of a common S haplotype BnS-6 through genetic transformation. BnS-1/BnS-6 and BnS-7/BnS-6 were the main S haplotypes in 523 B. napus cultivars and inbred lines. The expression of SRK in different S haplotypes was normal (the expression of SCR in the A subgenome affected the SI phenotype) while the expression of BnSCR-6 in the C subgenome had no correlation with the SI phenotype in B. napus. The BnSCR-6 protein in BnSCR-6 overexpressed lines was functional, but the self-compatibility of overexpressed lines did not change. The low expression of BnSCR-6 could be a reason for the inactivation of BnS-6 in the SI response of B. napus. This study lays a foundation for research on the self-compatibility mechanism and the SI-related breeding in B. napus.

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“…Recognition specificity is achieved by interaction of the female determinant SRK with its ligand, the male determinant SP11 (Chapman and Goring, 2010). The interaction between SP11 and SRK triggers the signaling cascade in an S- haplotype-specific manner and results in the rejection of self-pollen, but the signal components involved are still not well characterized (Zhang et al , 2011). S-locus glycoprotein (SLG) encoded by the SLG gene is the second female determinant involved in SI reaction.…”

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confidence: 99%

“…Theoretically, B. napus lines are considered to be homozygous at two S loci, with one S haplotype being derived from B. rapa and the other from B. oleracea . Brassica napus is considered to be self-incompatible like its two ancestors and artificially produced lines, although most cultivated lines are self-compatible (Zhang et al , 2011). Most B. napus contain a class I S haplotype (similar to S 47 of B. rapa )intheAgenome and a class II haplotype (similar to S 15 of B. oleracea ) in the C genome (Okamoto et al , 2007; Zhang et al , 2008a).…”

mentioning

confidence: 99%

“…Not surprisingly, in homozygous plants with two recessive S haplotypes, the character of the recessive S haplotype appears as an SI phenotype that is usually a mutant phenotype. However, genetic changes in polyploid B. napus , including chromosomal rearrangements (Udall et al , 2005) and epigenetic phenomena (Gaeta et al , 2007; Zhang et al , 2011), can alter gene expression and phenotype. No original Czech hybrid cultivar based on SI has yet been released, but SI is currently intensively used in Czech breeding programs.…”

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confidence: 99%

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Detection of self-incompatible oilseed rape plants (Brassica napus L.) based on molecular markers for identification of the class I S haplotype

et al. 2014

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The selection of desirable genotypes with recessive characteristics, such as self-incompatible plants, is often difficult or even impossible and represents a crucial barrier in accelerating the breeding process. Molecular approaches and selection based on molecular markers can allow breeders to overcome this limitation. The use of self-incompatibility is an alternative in hybrid breeding of oilseed rape. Unfortunately, stable self-incompatibility is recessive and phenotype-based selection is very difficult and time-consuming. The development of reliable molecular markers for detecting desirable plants with functional self-incompatible genes is of great importance for breeders and allows selection at early stages of plant growth. Because most of these reliable molecular markers are based on discrimination of class I S-locus genes that are present in self-compatible plants, there is a need to use an internal control in order to detect possible PCR inhibition that gives false results during genotyping. In this study, 269 double haploid F2 oilseed rape plants obtained by microspore embryogenesis were used to verify the applicability of an improved PCR assay based on the detection of the class I SLG gene along with an internal control. Comparative analysis of the PCR genotyping results vs. S phenotype analysis confirmed the applicability of this molecular approach in hybrid breeding programs. This approach allows accurate detection of self-incompatible plants via a different amplification profile.

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Progress on characterization of self-incompatibility in Brassica napus L.

Cited by 4 publications

References 87 publications

Self-(in)compatibility inheritance and allele-specific marker development in yellow mustard (Sinapis alba)

Self-(in)compatibility inheritance and allele-specific marker development in yellow mustard (Sinapis alba)

Characterization of a Common S Haplotype BnS-6 in the Self-Incompatibility of Brassica napus

Detection of self-incompatible oilseed rape plants (Brassica napus L.) based on molecular markers for identification of the class I S haplotype

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