Progress report on an assessment of laboratory procedures suggested as indicators of protein quality in feedingstuffs

Boyne, A. W.; Carpenter, K. J.; Woodham, A. A.

doi:10.1002/jsfa.2740121206

Cited by 63 publications

(23 citation statements)

References 33 publications

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“…An analysis of the results of biological assays of the same products by biological and microbiological methods gives evidence for their comparatively good agreement which is in accord with the pertinent literature (BOYNE et al, 1961;BUNYAN et al, 1964;WATERWORTH, 1964;BENDER, 1969). The biological and microbiological assays yielded good agreement with respect to the NPU and BV.…”

Section: Discussionsupporting

confidence: 76%

Comparative Characterization of Microbiological and Biological Assays of Protein Quality of Some Foodstuffs

Kharatyan¹,

Kerimova²,

Ignatyev³

et al. 1974

Nahrung

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In order to verify the possibility of comparing the results derived from microbiological and biological assays, the nutritive value of the amino acid fortified noodle and potato grits was determined. The experiments were carried out in parallel on 54 rats-weanlings, using Tetrahymena pyriformis as the test-organism. An analysis of the results obtained by the two methods showed their good concurrence. Direct correlation between the food consumption, gain in body weight, values of FER, PER, RV, NPU, the content of total nitrogen in blood, albumins in the serum of rats and BV and RNV of Tetrahymena was established (with an exception of I -z cases). Best accord of the results obtained by the two methods was detected in studies of foodstuffs with a high biological value. The sensitivity of the microbiological assay decreased with a reduction of protein quality. Therefore, the microbiological method using Tetrahymena pyrifovmis can be applied in industrial laboratories only to the control over the changes of a relative biological value of foodstuffs a t different technological stages. During the development of new foodstuffs the microbiological method of assaying quality of a protein must be supplement with the biological method and determination of the degree of disagreement of the results obtained by the two methods.The nutritive value is the basic index of the applicability of a foodstuff for human nutrition. This is the reason why methods of determining the nutritive value are constantly in the focus of researchers. However, they encounter great problems when assessing diet quality in direct human experiments and, therefore, use rats and, less frequently, mice, chicks, dogs, pigs, monkeys in their experimentation. The number of investigations carried out on young rats-weanlings which are good enough models of man is particularly large (BONSENBIANTE et al., 1969). At the same time, the complexity of balance experiments with a large amount of animals involved makes the biological method hardly applicable for a rapid evaluation of the nutritive value of a great number of food samples which is an urgent need of the modern food industry. It needs express-methods and the microbiological assay seems to be the most promising in this respect. Its advantagesinclude low time, cost and tested material penalty; the use of this method makes it unnecessary to have a vivarium, allows reliable standardization of experimental conditions and, consequently, more accurate reproduci-17 Die Nahrung, 18. Jhg., Heft 3

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Section: Discussionsupporting

confidence: 76%

Comparative Characterization of Microbiological and Biological Assays of Protein Quality of Some Foodstuffs

Kharatyan¹,

Kerimova²,

Ignatyev³

et al. 1974

Nahrung

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“…I shows that for meat meals and fish meals there is no useful correlation between PQI and GPV, but with whalemeat meals the correlation is good. 2 The nitrogen solubilities of a number of groundnut meals, cottonseed meals, soya-bean meals and sunflower seed meals are shown in Tables 111-VI respectively. Table I1 records the results of Lund-Sandstrom fractionation for a number of groundnut meals, cottonseed meals and soya-bean meals, and it is at once apparent that fractions 111, IT and V of the cottonseed meals all correlate with GPV to some extent.…”

Section: Resultsmentioning

confidence: 99%

Prediction of quality in protein concentrates by laboratory procedures involving determination of soluble nitrogen

Barnes¹,

Woodham²

1963

J Sci Food Agric

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109 ConclusionsThe synthetic chelating agents used were resistant to nitrification in soils. Application of the acid chelates to soil can considerably modify the concentration of Under the conditions of the experiments described the mineral composition The effect on nutrients in plants. of cabbage plants was considerably more influenced than that of wheat plants. metal concentrations was more marked under alkaline than under acid soil conditions. AcknowledgmentPh.D. in the University of London.This work forms part of a thesis submitted by one of the authors (I. A.) for the degree of Wye College Nr. Ashford Kent

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“…The nitrogen solubility in 0-02 N-NaOH was determined using a method of Lyman, Chang & Couch (1953) as modified by Boyne et al (1961).…”

Section: Nitrogen Solubilitymentioning

confidence: 99%

Evaluation of protein quality of poultry feedingstuffs. 2. Fish meal

1980

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SummaryThe present study was made to evaluate the protein quality of 11 samples of fish meal (FM) covering a wide range of nutrient content to find a suitable laboratory method for predicting nutritive quality. The nutrient content of the samples ranged from 34 to 62% in crude protein, 28 to 48% in true protein and 0·99 to 2·87% in available lysine. The true protein content of FM was further partitioned into myogen, myosin and stroma proteins. The stroma protein accounted for 61% of total crude protein suggesting that a large portion of FM comprised scales, viscera, cartilage, bones and other inedible parts of fish (head, tail, etc.). Neither true protein nor its different fractions was found suitable to detect differences in protein quality of FM although there was an inverse relationship between stroma protein and nitrogen solubility.Available lysine content and relative nutritive value (RNV) were found accurate and reliable laboratory tests for assessing the protein quality of FM. The linear equations to predict gross protein value (GPV) from available lysine content and RNV areGPV = 11·2×% available lysine+34·5;GPV = 0·55×RNV+51.The correlation coefficients between biologically assayed and predicted GPV from available lysine and RNV were 0·71 and 0·62, respectively. The findings suggest that the screening of FM samples for their nutrient content is essential before compounding diets for different categories of chickens.

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Progress report on an assessment of laboratory procedures suggested as indicators of protein quality in feedingstuffs

Cited by 63 publications

References 33 publications

Comparative Characterization of Microbiological and Biological Assays of Protein Quality of Some Foodstuffs

Comparative Characterization of Microbiological and Biological Assays of Protein Quality of Some Foodstuffs

Prediction of quality in protein concentrates by laboratory procedures involving determination of soluble nitrogen

Evaluation of protein quality of poultry feedingstuffs. 2. Fish meal

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