Progress with Proteome Projects: Why all Proteins Expressed by a Genome Should be Identified and How To Do It

Wilkins, Marc R.; Sanchez, Jean‐Charles; Gooley, Andrew A.; Appel, Ron D.; Humphery‐Smith, Ian; Hochstrasser, Denis F.; Williams, Keith L.

doi:10.1080/02648725.1996.10647923

Cited by 1,100 publications

(518 citation statements)

References 127 publications

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“…11,12 The term 'proteomics' was first used in 1995 and was defined as the large-scale characterisation of the entire protein complement of a cell line, tissue or organism. 13 Generally, there are two strategies for proteomics: protein expressional proteomics and functional proteomics. 14 The former identifies the entire protein expression level in samples via high throughput technology designed to generate a global view of the organism's biology.…”

Section: Introductionmentioning

confidence: 99%

“…This approach allows a selected group of proteins to be characterized and can provide important information about protein signalling, post-translational modification (PTM), protein-protein interactions and disease mechanisms. 13,15 In male reproductive biology, proteomics is emerging as a tool for defining specific protein profiles. Searching for terms such as 'testis proteome' or 'sperm proteome', one finds more than 150 publications in the National Centre for Biotechnology Information PubMed database (http://www.ncbi.nlm.nih.gov/pubmed/).…”

Section: Introductionmentioning

confidence: 99%

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Proteomics of spermatogenesis: from protein lists to understanding the regulation of male fertility and infertility

Huang¹,

Sha²

2010

Asian J Androl

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Proteomic technologies have undergone significant development in recent years, which has led to extensive advances in protein research. Currently, proteomic approaches have been applied to many scientific areas, including basic research, various disease and malignant tumour diagnostics, biomarker discovery and other therapeutic applications. In addition, proteomics-driven research articles examining reproductive biology and medicine are becoming increasingly common. The key challenge for this field is to move from lists of identified proteins to obtaining biological information regarding protein function. The present article reviews the available scientific literature related to spermatogenesis. In addition, this study uses two-dimensional electrophoresis mass spectrometry (2DE-MS) and liquid chromatography (LC)-MS to construct a series of proteome profiles describing spermatogenesis. This large-scale identification of proteins provides a rich resource for elucidating the mechanisms underlying male fertility and infertility.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Proteomics of spermatogenesis: from protein lists to understanding the regulation of male fertility and infertility

Huang¹,

Sha²

2010

Asian J Androl

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“…Proteomics, as a technology, was conceptualized and defined in terms of studying all proteins by an Australian scientist Marc Wilkins (Wilkins et al 1995). Explosive growth of proteomics and its applications in biological sciences triggered discussion on its original definition to have a more inclusive definition of proteomics .…”

Section: Proteomics Technology and Its Applications In Plantsmentioning

confidence: 99%

Plant proteomics in India and Nepal: current status and challenges ahead

Deswal¹,

Gupta²,

Dogra³

et al. 2013

Physiol Mol Biol Plants

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Plant proteomics has made tremendous contributions in understanding the complex processes of plant biology. Here, its current status in India and Nepal is discussed. Gelbased proteomics is predominantly utilized on crops and noncrops to analyze majorly abiotic (49 %) and biotic (18 %) stress, development (11 %) and post-translational modifications (7 %). Rice is the most explored system (36 %) with major focus on abiotic mainly dehydration (36 %) stress. In spite of expensive proteomics setup and scarcity of trained workforce, output in form of publications is encouraging. To boost plant proteomics in India and Nepal, researchers have discussed ground level issues among themselves and with the International Plant Proteomics Organization (INPPO) to act in priority on concerns like food security. Active collaboration may help in translating this knowledge to fruitful applications. Ground-level issues in agriculture, biodiversity and sustainabilityThe Green revolution of the sixties led agricultural practices in the Indian subcontinent to improve, and fulfil the everincreasing demand for plant-based foods. This was concomitant with the consumption of large doses of inorganic fertilizers and chemical pesticides to sustain crop productivity. Despite the transformation of India into a "bread basket", a negative aspect of the then generally beneficial model, was the erosion of crop biodiversity through increasing industrialization of agriculture. This also gave rise to other ecological problems, such as decreasing soil fertility, chemical pollution of land and water resources, pesticide poisoning, and pest infestation due to growing pest resistance to pesticides. Agricultural crops or domesticated plants went through cycles of selections depending on needs and important traits. The traits kept changing as per the demands of particular eco-geography and/or demography, while the cycle of selection continued with limited number of genotypes in particular crop species. This practice resulted in a narrow primary or secondary gene pool of crop plants and their related species causing breeding depression, low polymorphism, and allied problems. Therefore, harnessing the available diversity only within crossable genotypes and/or species now limits conventional breeding. In fact, agriculture on the Indian subcontinent is once again at the crossroads. Further development of sustainable solutions will require deeper understanding of traits at molecular level that are critical in exploring and exploiting the existing molecular diversity in native plants. Molecular understanding of fertility barriers would be equally critical to utilize the well characterized, wider germplasm collections readily available for cereal crops.Managing biotic and abiotic stresses in crop production remains a huge challenge. This is compounded with the perpetually rising numbers of pests and pathogens due to changing climate and pest/pathogen adaptation to chemicals. Harnessing the existing biodiversity in crop plants using their proximate specie...

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“…This discipline has been evolving and developing since the last 20 years [1]. Proteomics now involves the more complex studies of the total set and structure of proteins, their PTMs, and their interactions, thereby opening up a new postgenomic era and opportunities in cancer research.…”

Section: Introductionmentioning

confidence: 99%

Proteomics in human cancer research

Pastwa

Somiari

Czyż

et al. 2007

Proteomics Clinical Apps

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Proteomics is now widely employed in the study of cancer. Many laboratories are applying the rapidly emerging technologies to elucidate the underlying mechanisms associated with cancer development, progression, and severity in addition to developing drugs and identifying patients who will benefit most from molecular targeted compounds. Various proteomic approaches are now available for protein separation and identification, and for characterization of the function and structure of candidate proteins. In spite of significant challenges that still exist, proteomics has rapidly expanded to include the discovery of novel biomarkers for early detection, diagnosis and prognostication (clinical application), and for the identification of novel drug targets (pharmaceutical application). To achieve these goals, several innovative technologies including 2-D-difference gel electrophoresis, SELDI, multidimensional protein identification technology, isotope-coded affinity tag, solid-state and suspension protein array technologies, X-ray crystallography, NMR spectroscopy, and computational methods such as comparative and de novo structure prediction and molecular dynamics simulation have evolved, and are being used in different combinations. This review provides an overview of the field of proteomics and discusses the key proteomic technologies available to researchers. It also describes some of the important challenges and highlights the current pharmaceutical and clinical applications of proteomics in human cancer research.

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Progress with Proteome Projects: Why all Proteins Expressed by a Genome Should be Identified and How To Do It

Cited by 1,100 publications

References 127 publications

Proteomics of spermatogenesis: from protein lists to understanding the regulation of male fertility and infertility

Proteomics of spermatogenesis: from protein lists to understanding the regulation of male fertility and infertility

Plant proteomics in India and Nepal: current status and challenges ahead

Proteomics in human cancer research

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